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M S Drozhevkina

Publications and source records attributed to M S Drozhevkina.

16 recordsLinked to original sources
PubMed1985

[Substantiation of the necessity of developing a single set of phages for serotyping of Vibrio cholerae].

A single set of phages for typing different V. cholerae biotypes has been developed in the USSR and proposed for use instead of two sets of phages intended for this purpose. This set has been developed in view of the following facts: the similarity of the biological properties of V. cholerae biotypes and the absence of absolute criteria for their differentiation; the existence of the foci of cholera with both biotypes circulating there; the probability experimentally confirmed, of the conversion of one biotype into the other.

Bacteriophage Typing↗
PubMed1984

[Lysogeny studies of Vibrio cholerae NAG].

To establish the presence of phages specific for V. cholerae NAG, 673 strains, 166 water samples from ponds and sewage and 674 fecal samples from patients with acute intestinal diseases have been studied; as a result, 54 phage races have been isolated. The occurrence of true lysogeny in V. cholerae NAG has been found to be infrequent (0.9-10% among newly isolated strains). Pseudolysogeny has been detected in 0.75% of the strains under study. Of the total number of truly lysogenic strains of V. cholerae NAG, 10% have been found to carry V. cholerae phages of types I, III, V, VII, IX. The possibility of the simultaneous existence of V. cholerae phages and V. cholerae NAG phages in the environment has been shown. Of the isolated phages, 45 races have no serological relationship with V. cholerae phages, while possessing specific lytic activity with respect to V. cholerae NAG.

Acute Disease↗
PubMed1982

[Characteristics of the cholera vibrios circulating in the 7th pandemic in different territories].

The study of 491 V. cholerae strains isolated from humans in the endemic foci and the areas of the spread infection during the period of 1969-1979 revealed no essential differences in the properties of the vibrios. The majority of the strains proved to be typical in their main biological properties and belonged to biotype E1 Tor, seroval Ogawa. Among E1 Tor strains a comparatively small number of cultures with changed properties were detected. In some states of India classical cholera vibrios and E1 Tor vibrios were found to circulate simultaneously.

Asia↗
PubMed1980

[Lysogenic conversion of cholera vibrios].

As a result of ultraviolet irradiation and treatment with nitrosmethyl urea, mitomycin C, ascorbic acid and sarcolysin, the elimination of prophage from wild lysogenic Vibrio cholerae strains, biotype El Tor, has been achieved. The elimination of prophage was accompanied by a change in the biological properties of the vibrios, and some of these biological properties were found to be correlated with the lysogenization of Vibrio cholerae by the definite races of temperate phages. Only a few races of type II phages, whose genome was responsible for the serological characteristics of the strain, its sensitivity to cholera diagnostic phages and polymyxin, were capable of conversion.

Ascorbic Acid↗
PubMed1979

Basic criteria and classification scheme for bacteriophages acting against cholera vibrios of the classical and El Tor biotypes.

In accordance with the principles, nomenclature and taxonomic tests recommended by the TCNV for the systematization of viruses, cholera and El Tor phages were classified in all categories of the taxonomic hierarchy. On the basis of this classification, all known cholera bacteriophages could be affiliated to the biological type Vira, sub-type Deoxyvira, class 2/D B -- Deoxybinala, family Phagoviridae, genus Phagovirus vibrio and species Phagovirus cholerae. Phagovirus cholerae includes homologous series III-D, IV-D and V-D with 10 serologic types representing the smallest taxonomic units. At same time, phages of the remaining families with a single-strainded structure of DNA and RNA have so far not been detected for cholera vibrios.

Antigens, Bacterial↗
PubMed1979

Phage typing of Vibrio cholerae using a new collection of phages.

The authors formed a collection of typing phages in order to differentiate cholera vibrios of both biotypes. The collection consists of 7 phages proposed by Mukerjee for typing vibrios of the classical biotype and 3 El Tor phages isolated at the period of the 7th pandemy of cholera. In forming the typing collection the authors observed the following principles: 1) to crate a single set of typing phages and a single scheme of phage type differentiation of classical cholera and El Tor vibrios; 2) to use virulent phages; 3) to form a collection of serologically different phage types. The proposed collection allows to reveal a larger number of phage types as compared with phages proposed by Mukerjee phage typing a larger number of strains enhancing thus the epidemiological significance of the method of phage typing cholera vibrios.

Bacteriophage Typing↗
PubMed1976

[Phage typing of cholera vibrios with different sets of phages].

A comparative study was made of two sets of Mukerjee and Drozhevkina-Arutyunova's bacteriophages in typing 514 strains of the El Tor vibrios and 45 strains of clasic biotype. It was shown that the Mukerjee or Drozhevkina-Arutyunova's phages could be used for the typing of cholera vibrios. The phages of the latter set prove to detect more phage types (18 against 11); they determine both the phage type and the biotype at the same time. The typing of cholera vibrios of both biotypes is possible, and the percentage of nontyping strains left is comparatively low (5.2 against 23.5 after Mukerjee). A table of the phage correspondence was made; it permits to obtain comparable data in using any set of the typing phages.

Bacteriophage Typing↗
PubMed1976

[Polyvalent diagnostic cholera phage].

A polyvalent diagnostic bacteriophage for rapid identification (in 6-10 hours) of cholera vibrio of both biotypes has been constructed of virulent E1 Tor phages and recommended for laboratory practice. Polyvalent diagnostic bacteriophage is strictly specific, inactive against the nonagglutinating vibrios, microbes of enteric group and those closely affiliated.

Bacteriophage Typing↗