Biomedical subjects
Publications and source records attributed to M S Graziani.
A chemical procedure for high-density lipoprotein (HDL) separation in normo- and hyperlipidemic sera has been developed. The procedure is effective up to 25 mmol/L of serum triglycerides and the constituents of the precipitation mixture (dextran sulfate, PEG-6000 and MgCl2) do not interfere with enzymatic assay of the HDL cholesterol.
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We report a rare finding: IgM kappa and Bence Jones lambda double gammopathy in serum of a 80-year-old man with untreated symptomatic multiple myeloma. The unusual findings are confined to the laboratory studies demonstrating also a Bence Jones lambda proteinuria, high erythrocyte sedimentation rate (113 mm/h), and anemia. The synthesis of the different light chains seems to occur in separate cellular clones.
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Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
A new chemical procedure for selective sequential separation of serum lipoproteins is described. Low density lipoproteins (LDL) were precipitated by polyvinylsulfate and the precipitates were redissolved in NaCl 0.45 mol/l. Very low density lipoproteins (VLDL) were precipitated from LDL-free supernatants by PEG-6000 at 100 g/l final concentration, pH 10, and the precipitates were redissolved in NaCl 0.15 mol/l. For the precipitation of high density lipoproteins (HDL), apolipoprotein B-free supernatants were treated by PEG-6000 at 266 g/l final concentration and the precipitates were redissolved in NaCl 0.15 mol/l. The procedure was effective up to a serum triglyceride concentration of 4.0 mmol/l as well as in serum samples without chylomicrons or remnants. The determination of cholesterol and triglyceride contents in separated lipoproteins allows the study of their chemical composition in normo- and moderately hypertriglyceridemic serum samples.
The aim of this study was to evaluate the ability of plasma levels of apo A-I and apo B to discriminate between male patients with and without angiographically determined coronary artery disease (CAD) in comparison to the levels of cholesterol, HDL-cholesterol and triglycerides. The plasma apo A-I and B levels were measured by a radial immunodiffusion assay that made use of well characterized monoclonal antibodies. Univariate statistical analysis showed that the mean values for HDL-cholesterol and apo A-I were significantly lower in patients with single-, double- and triple-vessel disease than in the control subjects. The mean values for plasma cholesterol and apo B were significantly higher in the group with double- and triple-, but not single-vessel disease, than in the group without CAD. No statistically significant difference among groups was found for plasma triglyceride values. Since the considered variables failed to assess the severity of the disease, as determined by the number of vessels involved, the 3 groups of patients with CAD were combined into one group. A multiple logistic analysis, performed in order to assess the independent role of the variables and to estimate the corresponding odds ratio, indicated an independent association of HDL-cholesterol, apo A-I and apo B with CAD. When the multiple logistic analysis was repeated with a stepwise procedure, only apo A-I and B entered into the model. The results of our study indicate that plasma levels of apo A-I and B are more useful than plasma lipid in detecting the presence or absence of coronary artery disease in a group of male patients undergoing coronary angiography.
We evaluated a new liquid homogeneous assay for the direct measurement of high density lipoprotein cholesterol (HDL-C Plus) in seven laboratories. The assay includes two reagents which can be readily used in most available clinical chemistry analyzers. The total CVs of the new method were below 4.6% and the bias in relation to the designated comparison method was below 3.9%. The total error ranged between 4 to 7%. HDL-C values determined by this method were in good agreement with those obtained by the old homogeneous assay using lyophilized reagents, and other homogeneous and precipitation assays (0.944 < r < 0.996). The assay was linear up to at least 3.89 mmol/l HDL-C. Hemoglobin did not interfere, whereas in icteric samples slight deviations were observed. Lipemia up to 11.3 to 22.6 mmol/l triglycerides did not interfere with this homogeneous HDL-C assay. In samples of patients with paraproteinemia, discrepant results were seen. This liquid homogeneous HDL-C assay was easy to handle and produced similar results in all laboratories participating in this study. This method will enable clinical laboratories to reliably measure HDL-C for risk assessment of coronary heart disease.