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Biomedical subjects

M S Peters

Publications and source records attributed to M S Peters.

At least 73 records · Page 4Linked to original sources
PubMed1986

Spitz naevi and malignant melanomas of childhood and adolescence.

We reviewed 33 cases of Spitz naevus and 19 of malignant melanoma in patients aged 20 years or less who were evaluated at our institution from 1950 to 1975 and followed-up for up to 32 years. The histological findings were studied prior to review of clinical data. There were no malignant melanomas in patients less than 9 years old; almost half (15/33) of the Spitz naevi were in this age group. Among the 25 histological criteria evaluated in the 52 lesions, the most striking differences between malignant melanomas and Spitz naevi were a higher degree of pagetoid spread, cellular pleomorphism, nuclear hyperchromasia and mitotic activity in the malignant melanomas, and a more prominent spindle cell component in Spitz naevi. We stress the importance of cytological as well as architectural criteria in distinguishing between Spitz naevi and malignant melanomas and emphasize the pitfalls that may be encountered because of overlap in histological features between the two groups.

Adolescent↗
PubMed1986

Localization of human eosinophil granule major basic protein, eosinophil cationic protein, and eosinophil-derived neurotoxin by immunoelectron microscopy.

By utilizing the colloidal gold particle technique, we localized eosinophil granule major basic protein, eosinophil cationic protein (ECP), and eosinophil-derived neurotoxin (EDN) in human nasal polyp sections by immunoelectron microscopy. Sections stained with affinity chromatography purified rabbit anti-human major basic protein, and subsequently with gold colloidal particle-goat anti-rabbit IgG, showed gold particles predominantly within granule cores, and not within other eosinophil organelles, plasma cells, mast cells, lymphocytes, or neutrophils. Sections stained with anti-ECP or anti-EDN showed gold particles concentrated over the granule matrix with fewer particles centrally. Control sections treated with preimmunization sera showed no staining of cells or organelles. These results verify the localization of major basic protein to the crystalloid core of the human eosinophil granule and show that ECP and EDN reside in the granule matrix. This technique provides a means of accurately locating the sites of major basic protein, ECP, and EDN deposition and thus of identifying eosinophil degranulation patterns in human disease.

Blood Proteins↗
PubMed1985

Amyloid elastosis. A new cutaneous and systemic pattern of amyloidosis.

A patient with papulonodular cutaneous disease was studied until his death from progressive systemic disease. The elastic fibers in the skin, subcutaneous tissue, and serosae were coated with an amyloid-staining material in a unique pattern, as demonstrated by special stains and electron microscopy. Visceral and cutaneous blood vessels from autopsy tissue also showed amyloidosis, particularly in relation to the elastic fibers. These findings seem to represent a unique syndrome of amyloid disease.

Amyloid↗
PubMed1985

Atypical fibroxanthoma. A study with antibody to S-100 protein.

Fourteen biopsy specimens from ten patients with atypical fibroxanthoma were studied with the peroxidase-antiperoxidase method for binding of S-100 antibody. Six specimens from four patients were minimally positive. The S-100-positive cells were observed in greatest concentrations at the periphery of the lesions and were associated with perivascular inflammatory cells. By this method, it appears that there is not a Langerhans' cell type of atypical fibroxanthoma. The S-100 antibody can help differentiate desmoplastic juvenile melanoma and malignant melanoma from atypical fibroxanthoma.

Antibodies, Monoclonal↗
PubMed1985

Balloon cell malignant melanoma.

A patient was seen with a pigmented plaque of malignant melanoma and multiple surrounding skin-colored dermal and subcutaneous nodules. Histologic examination showed that the pigmented lesion represented the balloon cell variant of malignant melanoma and that the adjacent nodules were local metastatic lesions. This case, along with a review of the literature, illustrates that many balloon cell melanomas show only mild cellular atypia and minimal mitotic figures, although the tumor has a malignant course, with local or distant metastasis. A careful examination of all the sections of the balloon cell tumor is important.

Aged↗
PubMed1985

Cytophagic panniculitis and B cell lymphoma.

A 43-year-old woman had multiple, painful nodules of the legs and edema of both lower extremities for 3 years and leukopenia for 8 years. This histologic findings of lobular histiocytic panniculitis with "beanbag" cells were typical of cytophagic panniculitis. This case is unique in that B cell lymphoma developed in the spleen, as verified by immunoperoxidase staining, and demonstrates that the disease not only may be a progressive disease per se but also may be associated with nonhistiocytic malignancy.

Adult↗
PubMed1985

Neutrophilic urticaria.

We studied 241 consecutive skin biopsies from patients with urticaria. Ten of the 241 specimens showed inflammation of dermal venules primarily with polymorphonuclear leukocytes, without evidence of necrotizing vasculitis. We speculated that this finding might represent an early histological form of urticarial vasculitis. However, review of the clinical histories and laboratory data from these 10 cases showed that most were associated with dermographism, normocomplementaemia, and negative direct immunofluorescence. These findings suggest that urticarial lesions with the histological picture of neutrophils in vessel walls may imply the presence of physical urticaria.

Adult↗
PubMed1985

Cutaneous extraskeletal Ewing's sarcoma.

Extraskeletal Ewing's sarcoma has been recognized as being histologically indistinguishable from Ewing's sarcoma of bone. Although the occurrence of extraosseous Ewing's sarcoma in deep soft tissues has been well described, such cutaneous lesions are rare. We report the clinical history, histopathologic findings, and results of ultrastructural study of this rare variant occurring in a 12-year-old girl. She presented with a dermal and subcutaneous tumor of the left third toe, without osseous involvement. The lesion was composed of masses of round-oval cells, with perilobular fibrosis, focal hemorrhage, ill-defined pale cytoplasm containing glycogen, and absent pericellular reticulin. Ultrastructural study showed a monotonous cell population, with focal thickening of apposing membranes, high nuclear/cytoplasmic ratio, copious intracytoplasmic glycogen, and paucity of organelles except mitochondria. We present this case along with discussion of the literature and problems concerning differential diagnosis of small cell malignancies in the skin.

Bone Neoplasms↗
PubMed1985

Increased numbers of hypodense eosinophils in the blood of patients with bronchial asthma.

We investigated the density of blood eosinophils from patients with asthma using polyvinylpyrrolidone-coated silica gel (Percoll) discontinuous density gradient centrifugation of peripheral blood leukocytes. Ten patients with allergic asthma, 10 normal subjects, and 2 patients with the hypereosinophilic syndrome (HES) were studied. The density distribution profiles of eosinophils from normal subjects showed: (1) peaks at densities of 1.085 to 1.090 g/ml and (2) inflection points or nadirs near 1.082 g/ml, below which only 10% of eosinophils were found. On the basis of these results, we divided eosinophils into 2 subpopulations: normodense (greater than 1.082 g/ml) and hypodense (less than 1.082 g/ml). Densities of eosinophils from patients with asthma and HES peaked at 1.083 and 1.076 g/ml (mean values), respectively, significantly lighter than eosinophils from normal donors (p less than 0.005 and p less than 0.001, respectively). The proportions of hypodense eosinophils in patients with asthma and HES were 35 and 95%, respectively, and were significantly greater than that in normal donors (p less than 0.002 and p less than 0.001, respectively). The density distribution profiles of a normal subject were stable over time, but those of asthmatic patients varied with time. For the 22 participants, there was a positive correlation between log-transformed blood eosinophil counts and the percentage of hypodense eosinophils (r = +0.86, p less than 0.001). Similarly, for 15 of them, plasma eosinophil granule major basic protein correlated with the numbers of peripheral blood eosinophils (r = +0.92, p less than 0.0005) and hypodense eosinophils (r = +0.92, p less than 0.0005). Thus, a portion of the eosinophils in asthmatic patients and most of the eosinophils in patients with HES are hypodense.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1985

Facial edema and eosinophilia. Evidence for eosinophil degranulation.

Two patients had recurrent facial edema and peripheral blood eosinophilia. One patient showed a marked increase in the serum level of the eosinophil granule major basic protein. In both patients, skin biopsy samples showed nonspecific mononuclear cell inflammation with few eosinophils. However, immunofluorescence staining showed extracellular localization of the major basic protein within the dermis, similar to that previously shown in chronic urticaria and the recently described syndrome of episodic angioedema with eosinophilia. These observations provide further evidence that degranulation of eosinophils occurs in the skin and suggest that eosinophil mediators may play a role in the development of cutaneous edema.

Blood Proteins↗
PubMed1985

Calcium ionophore A23187 calcium-dependent cytolytic degranulation in human eosinophils.

The divalent cation ionophore A23187 is frequently used for studies of eosinophil degranulation. Nonetheless, the mechanism whereby A23187 induces degranulation in human eosinophils is still unclear. In the present experiments, A23187 caused human eosinophils to release a granule protein, eosinophil-derived neurotoxin (EDN) and a membrane-associated protein, Charcot-Leyden crystal (CLC) protein in a calcium and a concentration-dependent manner. However, A23187 at a concentration (1 microgram/ml) that caused 15% EDN release and 30% CLC protein release also produced release of the cytoplasmic enzyme lactic dehydrogenase (LDH) and loss of cell viability, both of which were calcium dependent. CLC protein release preceded EDN release and was detectable even at 15 min after the addition of 1 microgram/ml A23187, whereas EDN release occurred after a lag period of 30 min, and coincided with LDH release. At 1 microgram/ml A23187, neither the release of LDH nor the loss of viability occurred with purified neutrophils obtained in the same blood sample as a by-product of eosinophil purification. Electron microscopic examination demonstrated that exposure to A23187 for 15 min resulted in an increase and elongation of microridges on the cell surface, and exposure for 45 min caused cell disruption followed by extrusion of membrane-bound granules through breaks in the plasma membrane. Only once was granule exocytosis observed. These results indicate that A23187 treatment of eosinophils causes an initial release of membrane-associated CLC protein by a noncytolytic mechanism, and causes degranulation as a result of eosinophil lysis.

Blood Proteins↗
PubMed1984

Pacemaker contact sensitivity.

In a patient who had 4 cardiac pacemakers implanted and removed, pruritus, redness, and swelling of the skin overlying the pacemaker developed at intervals of 6 weeks to 17 months after insertion. Patch testing showed a 2+ reaction to titanium. The positive result of this test, the titanium case of the generator, and the history of multiple local reactions around the generator site pointed toward contact sensitivity to the pacemaker. Although a review of the literature indicates that this problem is rare, it is of extreme importance to the patient with pacemaker contact dermatitis.

Adult↗
PubMed1983

Localization of eosinophil granule major basic protein in chronic urticaria.

The role of the eosinophil in the pathogenesis of cutaneous diseases is not known. The eosinophil granule major basic protein (MBP), constituting the core and accounting for greater than 50% of the eosinophil granule, is toxic to helminths and mammalian cells. To determine whether eosinophil degranulation occurs in lesions of chronic urticaria, we performed an indirect immunofluorescence assay on sections of formalin-fixed, paraffin-embedded tissue, utilizing affinity chromatography-purified antibody to MBP. Twelve of 28 biopsies showed evidence of degranulation as judged by the deposition of MBP outside the eosinophil. The positive staining was of 3 types: (1) small blood vessel walls (5 patients), (2) dispersion of granular material (9 patients), and (3) focal or diffuse immunofluorescence of connective tissue fibers (11 patients). These results suggest a possible role for the cytotoxic molecule MBP in the evolution of lesions of chronic urticaria.

Adolescent↗
PubMed1983

Immunofluorescence identification of eosinophil granule major basic protein in the flame figures of Wells' syndrome.

An indirect immunofluorescence assay using formalin-fixed paraffin-embedded skin was performed on six biopsies from four patients with eosinophilic cellulitis (Wells' syndrome) to determine the extracellular localization of eosinophil granule major basic protein (MBP). Serial sections from each biopsy were treated with either affinity chromatography-purified antihuman-MBP or staphylococcal protein A purified rabbit IgG (control material). There was striking extracellular fluorescence localized to flame figures, and intracellular staining of eosinophils in all sections treated with anti-MBP as compared with controls. The pattern of MBP extracellular staining corresponded to the configuration of each flame figure (as verified by counterstain of the same section with haematoxylin and eosin). These findings show that MBP can be used as a marker for determining eosinophil degranulation and, because MBP is localized to flame figures, they suggest that MBP may play a pathogenic role in Wells' syndrome.

Adult↗
PubMed1983

Bronopol allergic contact dermatitis.

Bronopol (2-bromo-2-nitropropane-1, 3-diol) is an antimicrobial compound widely used as a preservative, primarily in cosmetic formulations. Analysis of patch tests performed on our patients revealed an incidence of 12.5% relevant positive results to 0.5% and/or 0.25% bronopol. This result reflects a history of prolonged use of bronopol-containing lubricants in our referral population of patients with different types of severe, extensive dermatitis. Contact sensitization to bronopol in this population is probably facilitated by abnormal cutaneous barrier function. Our findings emphasize the need for further clinical study of the potential for bronopol to produce contact sensitivity, and suggest caution with regard to its use in patients with dermatitis.

Adult↗
PubMed1981

Bowenoid papules of the penis.

The histologic finding of intraepithelial carcinoma of the penis presenting clinically as benign-appearing papules has been emphasized recently in the dermatology literature as bowenoid papulosis. We describe a patient who had multiple papules of the penile shaft, a biopsy specimen of which showed the typical histologic pattern of squamous cell carcinoma in situ. A review of the clinical morphologic range of intraepithelial carcinoma of the penis and the clinical resemblance to such conditions as lichen planus and condyloma acuminatum underscores the need to consider the possibility of squamous cell carcinoma in situ being expressed as bowenoid papules.

Bowen's Disease↗
PubMed1981

The histopathology of lupus erythematosus panniculitis.

Lupus erythematosus panniculitis is a clinical variant of lupus erythematosus in which the main pathologic process involves the deep corium and subcutaneous tissue. We reviewed twenty-nine cases of lupus panniculitis, as well as the cases previously reported in the literature. The histopathologic changes in lupus panniculitis are characterized by a lymphocytic panniculitis, hyaline degeneration of the fat, hyaline papillary bodies, and lymphoid nodular structures in the lower dermis and subcutaneous tissue. Direct immunofluorescence can be important in supplementing the histopathologic study of lupus panniculitis. Lesions of discoid lupus erythematosus are seen in 21% of cases. When this disorder exists in the absence of other typical cutaneous or systemic lesions, the diagnosis of lupus erythematosus has been questioned. We believe that the histopathologic findings of this entity are alone sufficient for a diagnosis of lupus panniculitis, even in the absence of cutaneous or systemic lesions.

Adult↗