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M S Sudman

Publications and source records attributed to M S Sudman.

5 recordsLinked to original sources
PubMed1974

Antigenic relationship between American and African isolates of Blastomyces dermatitidis as determined by immunofluorescence.

The antigenic relationship between American and African isolates of Blastomyces dermatitidis was investigated through the use of the fluorescent antibody technique. Preliminary results suggest that the American and African isolates studies are not antigenically identical. The American isolates share antigens with the African ones; however, it appears that they also possess distinct antigens. Additional studies are necessary to confirm these findings and to draw any conclusion whether the African isolates represent a serotype(s) of B. dermatitidis or a distinct species.

Africa↗
PubMed1973

Identification of the Prototheca species by immunofluorescence.

Studies were carried out to develop fluorescent antibody reagents for the identification of the Prototheca species and for their differentiation from morphologically similar fungi of various genera in formalin-fixed tissues. Antisera against representative isolates of P. filamenta, P. moriformis, P. stagnora, P. wickerhamii, and P. zopfii were produced in rabbits. Antiglobulins, labeled with fluorescein-isothiocyanate that intensely stained most cells of the homologous species, were selected for use as potential diagnostic reagents. By adsorbing the conjugates with selected heterologous cross-staining protothecae, reagents that were both sensitive and specific were obtained. Evaluation of the adsorbed conjugates with sections of tissue infected with protothecae, sections of tissue infected with morphologically similar fungi, and cultures of protothecae showed that these reagents are useful for the rapid and reliable identification of the Prototheca species.

Adsorption↗