CONTINUITY OF DNA SYNTHESIS IN ESCHERICHIA COLI.
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Biomedical subjects
Publications and source records attributed to M SCHAECHTER.
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Sud, I. J. (Tufts University School of Medicine, Boston, Mass.), and M. Schaechter. Dependence of the content of cell envelopes on the growth rate of Bacillus megaterium. J. Bacteriol. 88:1612-1617. 1964.-The amount of cell-wall and cell-membrane material was measured in Bacillus megaterium growing at different steady-state rates. On a dry weight basis, the content of wall hexosamine and diaminopimelic acid, and of membrane lipid phosphorus was found to be inversely proportional to the rate of growth. During the transition from slow to fast growth, the synthesis of envelope materials showed a lag of 20 to 30 min. Possible implications of these findings are discussed in terms of regulatory mechanism.
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Schaechter, M. (College of Medicine, University of Florida, Gainesville) and K. Santomassino. Lysis of Escherichia coli by sulfhydryl-binding reagents. J. Bacteriol. 84:318-325. 1962-Washed suspensions of gram-negative rods were lysed by low concentrations of some sulfhydryl-binding and oxidizing reagents, but not by reducing agents. Some kinetic aspects of this phenomenon were studied with p-chloromercuribenzoate and Escherichia coli B/r. Structures resulting from the action of this reagent consisted of impure cell walls. These could be purified by treatment with trypsin. Cell walls prepared mechanically and cell membranes obtained by lysing protoplasts were not overtly affected by this chemical.
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