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Biomedical subjects

M Sakaniwa

Publications and source records attributed to M Sakaniwa.

8 recordsLinked to original sources
PubMed1997

Analysis of extracellular matrix proteins in malignant chicken cell lines.

The adherent chicken cell line, MDCC-MSB1-41C, was highly transplantable and metastatic in vivo, compared with the parental non-adherent cell line MDCC-MSB1 from Marek's disease (MD) lymphoblastoid tumor. For clarification of differences in extracellular matrix proteins in MSB1-41C and MSB1 cells, examination was made of various components of extracellular matrix proteins. A detachment experiment indicated the protein(s) recognizing the Arg-Gly-Asp (RGD) sequence, the minimum structure required for recognition by the cell-surface receptors, is essential for the adherent character. Immunoblot assay using antibodies showed increased expression of fibronectin, fibronectin receptors, and vinculin on MSB1-41C cell lines. RGD-directed integrins mediate important cell-cell adhesive interaction and these interactions with extracellular matrix proteins may thus possibly be requisite for migration, proliferation and metastatic dissemination of MDCC-MSB1-41C cells. The RGD-containing peptide in the culture medium could cause detachment of cultured adhesive lymphoid leukosis LSCC-1104X5 cells from the dish too.

Amino Acid Sequence↗
PubMed1989

Management of defecation in spina bifida.

The main presenting complaint in defecational disorders in spina bifida is constipation. The principal cause of this complaint is hypofunction or paralysis of muscles in the pelvic floor as a result of the spinal cord injuries. To evaluate the present status of defecation, anorectal manometry with special reference to the anorectal pressure profile has been useful. The possibility of using anorectal manometry for the research of anorectal movement and participation of spinal cord was discussed.

Anal Canal↗
PubMed1989

Computerized analysis of anorectal manometry.

A computer system was designed and applied to anorectal manometry that allows pressure data to be analysed in various ways. Two kinds of applications were presented. Image analysis enabled anorectal movements to be visualized as a three-dimensional image. This technique was shown to be useful in studying the pathophysiology of chronic constipation in children. Radial variation analysis demonstrated that there was no significant radial difference in the anal-canal pressure.

Anal Canal↗
PubMed1985

[Bowel function of spina bifida].

Bowel function of the spina bifida was studied by clinical assessment and anorectal manometric studies. Anorectal pressure profiles were well correlated with clinical assessment but not with recto-anal inhibitory reflexes. Early training of the defecation is emphasized to obtain good bowel function.

Defecation↗
PubMed1981

The production of anomalous pancreaticobiliary ductal union in canine models.

For the purpose of resolving the mechanism of the ill-effect of refluxed pancreatic juice, due to the anomalous pancreaticobiliary ductal union, canine models were produced. Mongrel adult dogs and puppies were used. Pancreatico-cholecystostomy was performed on 5 adult dogs and 5 puppies. Pancreatico-choledochostomy was performed on 10 adult dogs and 5 puppies. In both procedures, the cylindrical dilatation of the choledochus resulted. The pathological changes in the choledochus were very similar to the cylindrical choledochal dilatation in the human. Elastic fibers diminished universally and in some cases they vanished completely. The changes in the papilla of the bile duct were very slight. There were no cases with hardened papilla. The pathological changes were greater in the pancreatico-cholecystostomy. Enzymatic analysis of pancreatic juice in bile was very important in explaining the etiology. Amylase was proven to be very high throughout the cases, over 10,000 IU/1 by enzymatic assay. Trypsin and elastase were proven to be activated in pancreatico-cholecystostomy, naturally assuming the existence of enterokinase. They were aso proved to be activated in the pancreatico-choledochostomy, without the existence of enterokinase. Though the mechanism was not clear, the activated proteases were the actual cause of the ill-effect in this anomaly. Thus we have succeeded in producing the ideal animal models of the reflux of pancreatic juice into the bile duct in adult dogs and puppies.

Animals↗
PubMed1978

Conditions for studying the exact pressure changes in the alimentary tract.

The following conditions must be observed if the actual pressure in the alimentary tract is measured and recorded with the aid of an open probe: 1. The probe must have a laterally placed opening, rather than a terminal one; 2. The opening must be as large as or larger than the internal diameter of the probe; 3. The internal diameter of the probe must be at least 1.2 mm or larger; 4. The pressure should not be measured with a nonperfusion technique if exact measurements of the intraluminal pressure are to obtained. One always has to use a perfusion, and the measured pressure depends on the speed of perfusion; 5. In order to be certain that the measured pressure corresponds to the actual pressure, one must ascertain whether the pressure will not change although the speed of perfusion is increased.

Anal Canal↗