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Biomedical subjects

M Sakate

Publications and source records attributed to M Sakate.

8 recordsLinked to original sources
PubMed2006

Calcium gluconate and sodium succinate for therapy of sodium fluoroacetate experimental intoxication in cats: clinical and electrocardiographic evaluation.

Sodium fluoroacetate (SFAC) or Compound 1080 is a potent rodenticide, largely used after 1946 for rodent and home pest control. The toxic effects of SFAC are caused by fluorocitrate action, a toxic metabolite, which has a competitive action with aconitase enzyme, leading to citrate accumulation and resulting in interference in energy production by Krebs cycle blockade. In the present study, domestic cats were intoxicated with oral doses of fluoroacetate (0.45 mg/kg). The intoxicated animals presented emesis, diarrhea with abdominal pain posture and an abdominal palpation, tachypnea, bilateral midriasis, hypothermia, hyperexcitability and convulsions. Blood gas analysis indicated decreased pH and bicarbonate levels. Serum ionized calcium was also decreased. ECG showed non-specific changes in ventricular repolarization and ventricular arrhythmias. The survival rate was 75% in the treated group with calcium gluconate and sodium succinate and 37.5% in the nontreated group.

Animals↗
PubMed1993

Effects of amitraz on motor function.

The effects of amitraz, a formamidine derivative, on motor function were studied in rats. Behavioural and neurochemical studies were performed. Results show that amitraz was able: 1) to decrease locomotion and rearing frequencies of rats and to increase their immobility time in an open-field; 2) to displace to the left the control dose-response curve constructed to apomorphine-induced stereotyped behavior; 3) to potentiate both pentobarbital sleeping time and amphetamine effects on open-field behaviour of rats and 4) to increase not only the whole brain levels of noradrenaline but also the striatal levels of dopamine. In relation to control animals, the pesticide also induced 5) a decrease in the striatal levels of homovanillic acid, 6) pesticide effects on open-field behaviour of rats were not antagonized by yohimbine administration, and 7) metoclopramide administration on amitraz pretreated rats antagonized not only amphetamine effects on open-field behaviour, but also apomorphine-induced stereotypy. These results are discussed in the light of the actions of amitraz on biogenic amines. It is suggested that the pesticide effects on motor function are consequences of the inhibitory effects on MAO activity, most probably through the increases produced on catecholamine levels within the central nervous system.

Amines↗
PubMed1989

Some behavioral effects of the pesticide amitraz.

Acute oral administration of the pesticide amitraz at the doses of 60 and 100 mg/kg (N = 10 per group) significantly decreased the rearing frequency of rats observed in an open field to 8 +/- 8 and 5 +/- 5, respectively, when compared to 28 +/- 5 for control rats treated with vehicle only. The same doses of amitraz (N = 10 per group) increased duration of immobility to 80 +/- 50 and 113 +/- 64 s, respectively, when compared to 113 +/- 64 s for the controls. Acute oral administration of amitraz (20, 60 or 100 mg/kg, N = 10 per group) significantly increased the convulsive threshold dose of rats for strychnine, picrotoxin and pentylenetetrazole. Amitraz administered ip to mice at the doses of 20, 60 and 100 mg/kg (N = 10 per group) significantly increased sleeping time in a dose-dependent manner to 96 +/- 26, 120 +/- 29 and 198 +/- 58 min, respectively, when compared to 45 +/- 15 min for control mice treated with vehicle only. These results indicate that amitraz produces a depressant effect on the central nervous system.

Analysis of Variance↗
PubMed1987

Polarography of Walker tumor submitted to radiotherapy.

A polarographic study of oxygen was done in 57 rats inoculated with Walker 256 tumor and platinum electrode implanted in muscle and in tumor. The goal of the research was the study of oxygen in tumor before and after irradiation. Tumor growth caused a decrease in tumoral oxygen. Oxygen was always lower in the tumor than in the muscle. Radiotherapy with 2000 rad (but not with 1000 rad) increased oxygen in the tumor.

Animals↗
PubMedDate not supplied

Antagonism produced by hypertonic nutrient solutions on the depressive effect of carbofuran on isolated guinea pig atria.

The action of hyperosmotic nutrient solutions on the depressive effects of carbofuran was studied on spontaneously contracting guinea pig atria. The force and frequency of contraction were recorded using an isotonic lever. Carbofuran 33.0 micrograms ml-1 produced a gradual depressive effect. The time for stabilization of the depressive effect was 6.0 +/- 2.3 min. After the depressive effect of carbofuran was established, NaCl, mannitol, or urea were introduced into the organ bath to increase the osmolarity of the nutrient solution to about 100 mosmol kg-1 of water. This resulted in a reversal of the contraction force and frequency to the initial levels. The addition of the osmotic agents at the same concentrations before the addition of 33.0 micrograms ml-1 carbofuran avoided its depressive effect on guinea pig atria.

Animals↗
PubMedDate not supplied

Developmental and behavioral effects of prenatal amitraz exposure in rats.

The effects of prenatal amitraz exposure on physical and behavioral parameters of rats were studied. Pregnant rats were orally gavaged with amitraz (20 mg/Kg) or with distilled water (1.0 ml/Kg) on Days 1, 4, 7, 10, 13, 16, and 19 of pregnancy. After birth, cross-fostering was performed thus generating the following groups: control pups nursed by control dams (CC); control pups nursed by treated dams (CE); treated pups nursed by treated dams (EE) and treated pups nursed by control dams (EC). Results show that compared to pups of Group CC, groups prenatally exposed to amitraz (EC and EE) showed decreased age of vaginal opening. Group EE also showed earlier fur development and a delay in incisor eruption compared to Group CC. The ages of pinna detachment, eye and ear opening, testes descent, and reflex development (surface righting and startle) were not affected by amitraz exposure. Offspring of group CE also showed earlier fur development. Rats of group EE had higher locomotor activity and rearing frequency and shorter immobility time compared to the rats of group CC when observed in an open-field 30 days after birth but not 60 and 90 days. No significant differences were found in open-field behaviors among the CC, CE, and EC groups. The present findings indicate that prenatal exposure to amitraz caused transient developmental and behavioral changes in the exposed rat offspring and suggest that further investigation of the potential health risk of amitraz exposure to developing human offspring may be warranted.

Animals↗