Biomedical subjects
Publications and source records attributed to M Sampson.
The costal and crural parts of the diaphragm were separately stimulated in anesthetized dogs. Stimulation of the costal part increased the dimensions of the lower rib cage, whereas stimulation of the costal part decreased the dimensions of the lower rib cage. It is concluded that the diaphragm consists of two muscles that act differently on the rib cage.
The ability of certain lipopolysaccharide (LPS) preparations to elevate cyclic adenosine monophosphate (cAMP) in mouse thymus cells in the presence of Ro 20-1724, 4-(3-butoxyl 4-methoxybenzyl)-2-imidazolidinone, was not related to the source of supply, bacterial strain, or method of extraction. Under the same conditions adenosine is a potent stimulator of thymus cell cAMP and is, of course, blocked by the further addition of theophylline. When theophylline was added to the LPS preparations with Ro 20-1724, the cAMP production was also blocked. These studies suggested that the observed stimulation of cAMP by LPS preparations was due to adenosine and/or its nucleotides present as contaminants.
In five normal subjects with pulsus paradoxus (change in systolic blood pressure greater than 10 mm Hg) induced by breathing through external inspiratory resistance, the change in systolic blood pressure was related to swings in esophageal pressure (change in intrapleural pressure [PpI]). Effects of hyperinflation (to 75 to 85 percent of vital capacity) were examined by adding expiratory resistances, and the influence of the configuration of the chest wall was studied by using "intercostal" and "abdominal" breathing patterns. There was a curvilinear relationship between the change in systolic blood pressure and the change in PpI, and hyperinflation was not necessary for the production of pulsus paradoxus. At the same change in PpI, there was no significant difference between "intercostal" and "abdominal" breathing, suggesting that pulmonary overdistention with tensing of the mediastinum is not an important etiologic mechanism. Analysis of sustained Muller's maneuvers suggested that the temporal breathing pattern may be important in determining the degree of paradox and may account for its variability in patients with a given degree of obstruction of air flow.
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The 15-min incorporation of 3H-thymidine (Tdr) and 3H-uridine (Ur) into nucleic acids of freshly isolated mouse thymocytes decreased steadily with time of preincubation at 37 degrees C. Sodium ascorbate at 5 mM prevented the decline of 3H-Tdr incorporation by preventing the decrease in its uptake. No such effect was noted on the incorporation of 3H-Ur, suggesting that ascorbate might be more specific for cells in or near the S phase of the cell cycle. The ene-diol group on the ascorbate molecule was required for this function, as ascorbyl-2-sulfate was ineffective and dehydroascorbate (DHA) reduced 3H-Tdr incorporation even further. Ascorbate was also inhibitory at a lower concentration (0.1 mM) or lower cell density. Thiols such as dithiothreitol or reduced glutathione seemed to act like 0.1 mM rather than 5 mM ascorbate. The inhibition by 0.1 mM ascorbate was presented by 0.1% bovine serum albumin (BSA), catalase or anaerobiosis. BSA had its own protective effects on the cells, since at 0.1% it increased the uptake of both 3H-Tdr and 3H-Ur. The combined effects of 5 mM ascorbate and 0.1% BSA on 3H-Tdr uptake were additive, but some synergism was noted at the lower BSA concentrations. These results suggest that with low concentrations of ascorbate (0.1 mM) oxidative reactions occur in vitro, resulting in the accumulation of the toxic hydroxyl radical (. OH). High concentrations apparently override this inhibition by a mechanism possibly involving an increase in critical cellular thiol groups.
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Regional cerebral glucose metabolism was surveyed in 37 Alzheimer's disease (AD) patients and 21 normal controls using positron emission tomography. Where possible, brain regions were specified according to their neurobehavioral function rather than as anatomically demarcated structures. Absolute metabolic values revealed significant differences (p < 0.05) between AD patients and controls for whole brain and the more superior supratentorial brain slices. Normalized values (region/brain stem) showed the most striking declines (p < 0.001) in the association cortex (heteromodal region -21%; unimodal region -19%) and the primary sensory-motor cortex (-13%), with motor, auditory, and visual areas more affected than somatosensory areas. Limbic and paralimbic systems were equally affected (-14%; -11%; p < 0.001). Thalamus, striatum, cerebellum and brain stem were minimally or not affected. Neurobehaviorally defined hypometabolic regions largely parallel affected areas noted in anatomic and previous metabolic studies, with the possible exception of metabolic deficits in the primary sensory-motor complex. Conceivably, brain areas unaffected morphologically by the pathophysiological processes of AD may become dysfunctional due to a disruption of connectivity between regions.
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On the occasion of Franz Halberg's 70th birthday, some of his many achievements are reviewed. We provide a historical background to the development of chronobiology; offer insight into the current state of this new science; and sketch the promise of this discipline for health care and cure. As a tribute to Franz Halberg, in an era of fast-growing technology, an attempt is made to describe his perspective of tomorrow's medicine and biology. The many students he trained throughout his productive career face the challenge of deserving the trust he placed in them and of further implementing his vision. A leader in social pediatrics put it aptly: it will take several generations of researchers to study and master his life's work.