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Biomedical subjects

M Sar

Publications and source records attributed to M Sar.

At least 163 records · Page 9Linked to original sources
PubMed1975

Estrogen target sites in the brain of the chick embryo.

Autoradiograms prepared from brains of chick embryos after injection of [3H]estradiol demonstrate the existence of target cells for estrogen in the medial preoptic and ventral hypothalamic regions as early as day 10 of incubation. Target cells also appear in telencephalic locations during later stages of embryonic development. These hormone-concentrating cells probably are the anatomical substrate for the formative action of sex steroids during embryonic life on certain brain functions such as control of sexual and aggressive behaviour and gonadotropin secretion.

Age Factors↗
PubMed1975

Simultaneous autoradiographic and immunohistochemical localization of estrogen and gonadotropin in the rat pituitary.

A combined autoradiographic and immunohistochemical technique is introduced which is suitable to simultaneously localize hypophyseal sites of nuclear (3-H)-estradiol uptake and anti-hCG-characterized "gonadotropes". Most, but not all, "gonadotropes" show significant nuclear concentration of radioactivity. A large percentage of non-"gonadotropes" also show nuclear uptake of label.

Animals↗
PubMed1975

Autoradiographic localization of radioactivity from [3-H]oxytocin in the rat mammary gland and oviduct.

[3-H]Oxytocin was incubated in vitro with pieces of oviduct and mammary gland and the tissue were subjected to autoradiography. Radioactivity was localized only in smooth muscle cells of the oviduct and in regions of mammary tissue where myoepithelial cells are found. In contrast, radioactivity was not concentrated in any region of skeletal muscle, a nontarget tissue for oxytocin. The localization of radioactivity in oviduct and mammary cells was absent when the tissues were incubated with [3-H]oxytocin and an excess of unlabeled oxytocin. These findings provide evidence for the presence of specific and high affinity receptors for oxytocin in its target tissues.

Animals↗
PubMed1975

Synergism between prolactin and ovarian hormones of DNA synthesis in rats mammary gland.

Autoradiography with [30H] thymedine has been used to measure the rate of DNA synthesis in the mammary epithelium of hypophysectomized-ovariectomized rats under the influence of estradiol, progesterone, and prolactin. Controls and animals treated with estradiol did not increase [3-H] thymidine incorporation, while progesterone alone had a slight stimulatory effect. Prolactin alone stimulated some [3-H] thymidine uptake in ductal and alveolar epithelium, but when combined with either estradiol or progesterone synergistic effects were observed. Estradiol with prolactin stimulated incorporation primarily in the ductal epithelium, whereas progesterone with prolactin stimulated both ductal and alveolar epithelium.

Animals↗