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Biomedical subjects

M Schreuder

Publications and source records attributed to M Schreuder.

5 recordsLinked to original sources

Isolation and characterization of mRNAs accumulated during in-vitro flower bud formation.

The development of vegetative and generative buds on thin-layer explants of tobacco (Nicotiana tabacum L. cv. Samsun) has been studied at the level of translatable mRNA to detect changes in the mRNA population during bud initiation and differentiation, and several quantitative differences were found. By differential screening of a cDNA library obtained from flower-bud-regenerating explants we have isolated a group of six cDNA clones representing genes that are preferentially expressed during in-vitro flower bud formation. Nucleotide sequence analysis of one of these cDNAs, pAP8, showed that the most likely open reading frame has some typical characteristics of, and homology with, extensin-like genes. Northern blot analysis and in-situ hybridization suggest a specific role for these extensin-like genes in flower bud initiation on tobacco pedicel explants.

Amino Acid Sequence↗

Anaesthesia without tears.

Rectal induction of anaesthesia is a useful method but is not widely used in South Africa. We studied the onset of action and side-effects of 1% methohexitone administered rectally in a dose of 20 mg/kg to 110 preschool children. Ninety-one per cent were adequately sedated for inhalation induction by mask within 10 minutes, and all by 15 minutes, of drug administration. There was no evidence of significant cardiovascular or respiratory depression and only minor complications such as faecal soiling (11.8%) and hiccough (3.6%) were noted. The technique has been favourably received by parents, surgeons and nursing staff and has now become routine practice.

Anesthesia, Rectal↗

Pain relief in herpes zoster.

The severity of pain as a symptom of herpes zoster and post-herpetic neuralgia has seldom been emphasized in the literature. In this report on a series of 113 patients, a treatment which gives immediate relief of pain and prevents post-herpetic neuralgia is described. Provided that the steroid solution could be placed accurately in the epidural space adjacent to the affected nerves, the method was 100% successful. Failure to provide relief of pain after the initial effect of the local anaesthetic had worn off was taken as an indication that the epidural injection had been misplaced, and it was repeated.

Adolescent↗