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M Sebald

Publications and source records attributed to M Sebald.

63 records · Page 4Linked to original sources
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Clostridium bifermentans serovar malaysia: sporulation, biogenesis of inclusion bodies and larvicidal effect on mosquito.

Sporulation of Clostridium bifermentans serovar malaysia, which has a larvicidal activity towards mosquitoes, was examined by electron microscopy. Parasporal inclusion bodies lacking a crystalline structure were first detected at t5 (5 h after the end of exponentional growth). Also, the presence of "brush-bottle"-like appendages appearing first at t5 was noted; these remained attached to the spores when released after sporangium lysis. Larvicidal activity assayed on Anopheles stephensi larvae appeared at t0 and increased rapidly to a maximum between t5 and t8. However, a decrease in bacterial toxicity occurred with sporangium lysis.

Animals↗
PubMedDate not supplied

Conjugal transfer of plasmid-mediated antibiotic resistance from streptococci to Clostridium acetobutylicum.

Three broad host-range MLS plasmids, pAM beta 1 (Em), pIP501 (Em, Cm) and pJH4 (Em, Sm, Km), were transferred by a conjugation-like process from Streptococcus faecalis to Clostridium acetobutylicum. The plasmids were stably maintained and antibiotic resistances were fully expressed in C. acetobutylicum, except for chloramphenicol resistance, which was poorly expressed, if at all. The C. acetobutylicum strains harbouring every plasmid could be used as donors in intraspecific matings with frequencies of approximately 1 X 10(-5) to 1 X 10(-6).

Anti-Bacterial Agents↗
PubMedDate not supplied

[Detection of "Clostridium difficile" on minimal and selective medium and by immunofluorescence antibody staining (author's transl)].

A minimal and selective medium containing 5 aminoacids, 3 vitamins, a low sugar concentration and 2 antibiotics (cefoxitin at 16 microgram/ml and streptomycin at 500 microgram/ml) is described for isolation of Clostridium difficile from the gut. Comparable results were obtained with this medium and using spore isolation by the sodium thioglycolate-lysozyme technique. An antiserum specific to C. difficile was prepared and used for detection by immunofluorescent antibody staining. This is a very sensitive technique which leads to a quick and early identification of this bacterial species.

Animals↗