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Biomedical subjects

M Sefer

Publications and source records attributed to M Sefer.

8 recordsLinked to original sources

[Studies of oral microbiocenosis. I. Incidence and properties of staphylocci in young persons].

The study of staphylococci in the buccodental washings of 117 second year students at the Faculty of Stomatology, all healthy youths aged 20-25 years, gave the following results: (I) stapphylococci were present in 90% of the students; (II) 50.66% were oral carriers of pathogenic staphylococcal strains; (III) in 23 cases two or more staphylococcal strains were found with different metabolic and biologic properties; (IV) part of the bucal lysozyme was of microbial origin since 95% of the coagulasopositive strains and 88% of the coagulasonegative strains produce lysozymes; (V) 35.23% of the strains yield staphylococcal active with regard to M. lysodeicticus; (VI) 56.19% of the staphylococcal strains were resistant to penicillin and/or tetracyclin, and 5 coagulopositive strains developed in media with a penicillin concentration of 800 u/ml; (VII) 85% of the coagulopositive staphylococci were sensitive to human anti-staphylococcal bacteriophages, the most frequent being phage type 77 of group III.

Adult

[New bacterial microorganisms in the etiology of human infections. The genus Gardnerella].

Haemophilus vaginalis first affiliated in the bacterial systems in the genus Haemophilus, the Corynebacterium due to its morphophysiological characteristics--forms a new genus Gardnerella with only one species G. vaginalis. The bacterium is important for man's pathology, as it is responsible for the most numerous vaginitis, considered nonspecific. The paper reports synthetically the present knowledge in biology, pathogenicity, diagnosis and treatment.

Animals

[10 years after the death of Professor Mihai Ciucă (18 July 1883--20 Feb 1969). Mihai Ciucă and the study of bacteriophage, recorded in his working notebooks].

The work records of professor Mihai Ciucă (found in the archives of the Department of Microbiology) give us an insight into his scientific thoughts and practical activity. These documents speak of his dedication to the problem of the bacteriophage started in Jules Bordet's laboratory of the Pasteur Institute in Brussels, in 1920 and continued until 1969, the year in which he died. Since the discovery of the phenomenon of lysogeny, called the Bordet-Ciucă phenomenon, the professor initiated a wide range of research themes on the bacteriophage, guiding and promoting the work of a great number of specialists in the National Center, founded in 1949.

Bacteriology

[Studies of oral microbiogenesis. II. Significance of staphylocci present in periodontal lesions, pulp gangrene and in the alveolus after tooth extraction].

A study was carried out on the properties and significance of staphylococci isolated from the alveoli after dental extraction (12 strains), from parodontal pus (19 strains) and pulpar gangrene (15 strains). By their frequency, staphylococci of the Staphylococcus aureus have an etiologic significance in pulpar gangrene. Of particular importance for the diagnosis and epidemiology was the predominance of lysotype 80 among the staphylococci isolated from pulpar gangrene and lysotype 77 in healthy carriers. In 2 of 11 patients the coexistence of the same lysotype was found in the pulpar gangrene and nasal secretion.

Alveolar Process

[Antibiograms of Leptospira on solid media in Petri dishes].

The sensitivity of fowl leptospirae to antibiotics was tested by diffusiometry on solid media in Petri dishes. The method includes two stages: 1) visible leptospirae cultures are obtained by central seeding and incubation during 3--5 days at 28 or 37 degrees C; 2) antibiotic tablets are disposed at 2 cm from the visible edge of the multiplication zone of the leptospirae. The edge is marked with a pencil at the moment in which the antibiotics are applied. Incubation is continued at the same temperatures. The results are read after five days taking as reference the position of the marginal multiplication line. The sensitivity of leptospirae to antibiotics is expressed by arrested multiplication and resistance by advance of the marginal multiplication line up to or even beyond the antibiotic tablets. Fowl leptospirae are sensitive to penicillin, tetracyclin, chloramphenicol, streptomycin, novobiocin, polymixin, pristinamycin, neomycin, erythromycin, and resistant to mycostatin, septrin, rifampicin, optoquine, sulphatiazol.

Anti-Bacterial Agents