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M Seifert

Publications and source records attributed to M Seifert.

At least 91 records · Page 5Linked to original sources
PubMed1992

[The value of adjuvant irradiation in lymphatic vessel invasion in patients with a cervical carcinoma in histopathological stage Ib and with negative lymph nodes].

The present retrospective study attempts to evaluate the significance of adjuvant radiotherapy as a prognostic factor for stage Ib cervical carcinoma without lymph node metastases but with lymph-vascular space invasion. 54 patients fulfilled the inclusion criteria: histopathological stage Ib, radical hysterectomy with pelvic lymphadenectomy, negative pelvic lymph nodes and lymph-vascular space involvement. Though larger tumors were found (tumor infiltration of the cervix greater than 2/3) in the irradiated group (chi 2-test p = 0.003), there was no significant difference in the five-year overall survival (92% five-year survival without vs. 90% with adjuvant irradiation). In contrast statistical significant difference was found in recurrence-free interval between these groups (Wilcoxon test p = 0.02).

Carcinoma↗
PubMed1992

Lymphocyte surface marker expression on hybridomas secreting human monoclonal antibodies.

The expression of human leucocyte markers on the surface of hybridoma cell lines producing human monoclonal antibodies was studied using immunofluorescence analysis (FACS). We tested 36 different hybridoma cell lines from fusions of lymphocytes of different organs of fetal and adult organisms with the mouse myeloma line P3 X63 Ag8.653 or the mouse-human heteromyeloma line CB-F7 (IgM-, IgG-, and nonproducer) with a panel of 21 murine monoclonal antibodies against human differentiation and activation antigens. CD2, 3, 4, 5, 8, 10, 23, 25 antigen and major histocompatibility complex (MHC) class II determinants could not be detected on all hybridomas analyzed. The antigens CD22, 69, 71, and 72 were expressed on few of the hybridomas tested. The majority of the cell lines carried the surface markers CD19, 20, 40, 45 as well as the plasma cell markers CD38 and O/C11. The activation antigen 4F2 was expressed on all the cell lines tested. However, a direct connection between the expression of a lymphocyte marker and the capacity for Ig production (high and low producer; Ig isotype), the origin of the lymphocytes, and the fusion cell line used could not be detected.

Antibodies, Monoclonal↗
PubMed1991

[Melanoma of the vulva. Report of 12 cases].

The present retrospective study includes 12 patients with melanoma of the vulva who underwent vulvectomy between 1977 and 1987. Six patients were less than 55 and 6 other patients more than 69 years old. Three women of the younger age group survived (31, 44 and 116 months, respectively), whereas three of them died of the tumour. In the patient group of over 69 years of age 4 patients died of the tumour, one patient did not appear for follow-up and one further patient died in the meantime. Nine patients suffered from deeply infiltrating tumours (Clark levels IV and V), 2 patients had a Clark level II tumour, and in one case the Clark level was unknown. Depth of infiltration correlated with the prognosis of disease, i.e. only one patient with deep tumour infiltration, but both patients with Clark level II tumours survived. Therefore, the importance of early diagnosis of melanoma of the vulva should be emphasized, and accurate inspection of the vulva should be indispensable in the course of routine gynaecological examination also in younger women.

Adult↗
PubMed1991

[Effect of various factors on survival after recurrent and/or metastatic breast cancer].

145 women (22.2%) out of 652 patients with primary breast cancer, who were operated on between January 1980 and September 1988, developed tumour relapse until May 1990. The aim of our retrospective analysis in these 145 patients with local and/or distant tumour relapse was to evaluate the prognostic importance for further survival of the following factors: menopausal status, stage of disease, number of involved axillary lymph nodes, tumour grading, ER and PgR content, postoperative irradiation, adjuvant treatment, localisation of tumor relapse and relapse-free interval. Multivariate stepwise regression analysis of all these factors identified the number of positive axillary lymph nodes (0-3 versus 4+) with a relative risk (RR) of 2.49 and localisation of tumour relapse (local versus visceral metastasis RR = 2.08 and local versus bone metastases RR = 2.08) as the only two significant prognostic factors for further survival. Therefore, these two factors should be stratification criterias for prospectively randomized phase III studies in patients with tumour relapse after primary breast cancer.

Adult↗
PubMed1991

[Radiotherapy of primary vaginal carcinoma and effects of histological and clinical factors on the prognosis].

434 cases of primary vaginal carcinoma were treated from 1950 to 1984 at the Irradiation Department of the University Clinic for Obstetrics and Gynecology, Vienna. The five-year survival rate for various clinical stages is as follows: Stage I 76.7%, stage II 44.5%, stage III 31%, stage IV 18.2%. In this retrospective study 110 patients seen from 1975 to 1984 were reviewed in detail and evaluated with a view toward their clinical and morphological aspects and survival rates. Patients up to the age of 60 have a five-year survival rate of 50%, those over 75 years of age reach only 34.3%. If the carcinoma was discovered in a routine check up the survival rate is 61.1%, whereas in cases with symptoms the rate decreases to 36.9%. Survival differences were also observed for patients with a carcinoma in the upper third of the vagina (61.1%) versus patients with a carcinoma in the lower third (33.3%); for patients with well-differentiated tumours (62.5%) versus patients with grades II and III (41.5% and 34.9%). It can be seen, that with brachytherapy local control of the carcinoma can be achieved with excellent results in early stages. Treatment of patients with more advanced carcinomas should include external beam therapy. The importance of gynecological screening especially for older women is emphasized.

Adenocarcinoma↗
PubMed1990

[Is consecutive curettage of the corpus uteri indicated in every conization case?].

Endometrial curettage at the time of conisation is often performed as a routine procedure, even when indication for this treatment is rather unclear. 593 conisations including curettages were performed at the I. Department of Obstetrics and Gynaecology, of the University of Vienna and were evaluated retrospectively. Only 3 patients with a mild endometrial hyperplasia were found. Therefore, in our opinion, there is no indication for a curettage at the time of conisation, if a woman is under the age of 35 years and if there is no other specific indication for sampling the endometrium, based on high risk factors, physical examination, history or abnormal glandular cells found on cervical cytology. Such management would result in reducing the costs and the risk of infection in intrauterine operations.

Adult↗
PubMed1990

[Monoclonal antibodies against determinants on human MHC class II antigens].

Monoclonal antibodies against different non-polymorphic determinants of HLA class II antigens are described. The epitopes are present on HLA-DR and HLA-DQ gene products as detected by monoclonal antibody BL-Ia/l, and at HLA-DR and HLA-DP gene products which are recognized by monoclonal antibody BL-Ia/4. Another epitope recognized by the monoclonal antibody BL-Ia/DQ is only found on DQ molecules. The monoclonal antibodies BL-Ia/1, BL-Ia/4, and BL-Ia/5 are directed against the beta-subunit of 29 kDa/35 kDa class II heterodimer molecules. The recognized epitopes are shown to be not dependent on the association of the alpha- and beta-chains.

Antibodies, Monoclonal↗
PubMed1990

[Characterization of murine monoclonal antibodies against the common acute leukemia antigen (CALLA)].

This study presents two murine monoclonal antibodies which react with the Common Acute Lymphoblastic Leukemia Antigen (CALLA). Both antibodies can be used for the diagnosis of common ALL (cALL). Indirect immunofluorescence studies (FACS-analysis) showed that the antibodies react with granulocytes and different human cell lines (Nalm-6, Reh, Raji, CCRF-CEM). The monoclonal antibodies BL-CALLA/1 and BL-CALLA/2 identify a single polypeptide chain of 95 kD. Both antibodies recognize the same or closely related epitope of the CALLA-molecule and are able to modulate in vitro the antigen on the CALLA-positive cell line Reh.

Animals↗
PubMed1989

[The importance of sonography for the acute diagnosis of multiple trauma].

Sixty polytraumatised patients with blunt abdominal injuries were sonographically examined immediately after hospitalisation, between January 1985 and October 1988. Accumulation of free fluid in the abdominal cavity or organ injuries were directly recorded from 17 patients. Results obtained from examinations were falsely negative in three cases. The method, consequently, exhibited 0.85 in sensitivity, 1.0 in specificity, and 0.33 in prevalence. Ultrasonography is recommended as the first step to be taken for diagnosis, preceded only by clinical check-up and parallel shock therapy. It may be followed by peritoneal lavage, yet only in the event of dubious findings.

Abdominal Injuries↗
PubMed1989

[A system for respiratory flow proportional sampling].

There is a method described for determination the amount of air pollutants incorporated by breathing. The method is based on the non-invasive and continuous sampling of air contents. In contrary to common methods there is no need for any assumptions about the retention rate. Working principle: The breathing flow drives two pneumatic circuits related to inspiration and respiration respectively. The respirometer signal controls the two membrane pumps and delivers the respiration volume. In every moment a little but fixed percentage of breathing air is sucked by a pump through one of the two charcoal tubes. After the quantitative analysis of the probes you know the mass difference of the pollutant and because of the choice relation to respiration volume you can calculate the incorporated dose.

Air Pollutants, Occupational↗
PubMed1984

The Ambulatory Sentinel Practice Network: purpose, methods, and policies.

The Ambulatory Sentinel Practice Network (ASPN) is a network of primary health care practices across the United States and Canada offering (1) a laboratory for the study of populations under the care of primary care providers, and (2) surveillance of primary care problems and services. This paper reports the methods and policies developed and used by ASPN to conduct studies and describes the initial sentinel practices.

Adolescent↗