PubMed Health⌕ Search

Biomedical subjects

M Senba

Publications and source records attributed to M Senba.

At least 37 records · Page 2Linked to original sources
PubMed1989

[A case report of giant cell granuloma of the jaw].

A giant cell granuloma of the mandible seen in a 16 month-old boy was reported. Histopathology showed that the lesion consisted basically of granulation tissue with multinucleated giant cells, spindle-shaped and polygonal stromal cells. No atypia was seen in giant cells or stromal cells. Electron microscopic examination revealed that multinucleated giant cells possessed microvillous structure on their cytoplasm and were rich in organelles such as endoplasmic reticulum, mitochondria and dense bodies. Stromal cells consisted of macrophage-like and fibroblastic cells. Immunohistochemical examination demonstrated a large number of giant cells and stromal cells that were strongly positive for the antibodies of alpha-1-antitrypsin and alpha-1-antichymotrypsin. These morphological findings suggest that macrophage-like stromal cells are the precursors to giant cells.

Giant Cells↗
PubMed1989

Osteoclast-type giant cell tumor of the endometrium. An immunohistochemical study.

We present a rare case of a 55 year old female with an osteoclast-type giant cell tumor of the endometrium associated with leiomyoma and adenomyosis. Multinucleated giant cells and mononuclear stromal cells reacted with vimentin and alpha-1-antichymotrypsin (AACT) using the immunoperoxidase method. Epithelial membrane antigen (EMA), carcinoembryonic antigen (CEA), and keratin exhibited negative immunoreaction in these tumor cells. Our immunohistochemical results do not support the epithelial origin of an osteoclast-type giant cell tumor and mesenchymal derivation appeared more likely, suggesting histiocytic origin.

Female↗
PubMed1989

Nature of yellow-brown bodies. Histochemical and ultrastructural studies on the brown pigment.

Yellow-brown bodies were observed in the sinusoids of lymph nodes and in histiocytes. The authors examined their histochemical characteristics. They were similar to lipofuscin pigments in the liver. However, ultrastructurally, yellow-brown bodies were different from lipofuscin and melanin pigments. The electron microscopic investigation suggested that degradation products of yellow-brown bodies in histiocytes resemble brown pigments in the black thyroid.

Histiocytes↗
PubMed1988

Eosinophilic globules in pheochromocytoma of the adrenal medulla. A histochemical, immunohistochemical and ultrastructural study.

Histochemical, immunohistochemical, and ultrastructural characteristics of eosinophilic globules in pheochromocytoma of the adrenal medulla are described. The globules were observed in 7 (63.5%) out of 11 cases of pheochromocytoma of the adrenal medulla. These globules were eosinophilic, PAS-positive with and without diastase predigestion, phosphotungstic acid hematoxylin (PTAH) positive, and autofluorescent under ultraviolet illumination. Although almost all globules were not stained with neuron specific enolase (NSE) using the immunoperoxidase method, a few globules were stained positive. The lectins of Triticum vulgaris (WGA) and Ricinus communis (RCA-120) were weakly bound to most of the eosinophilic globules. Immunohistochemical reactions for alpha-fetoprotein (AFP), alpha-l-antichymotrypsin (ACT), and human chorionic gonadotropin (HCG) revealed negative reaction in these globules. An ultrastructural study revealed no relationship between these globules and chromaffin secretory granules. These findings suggested that the eosinophilic globules in pheochromocytoma of the adrenal medulla were not related to the chromaffin secretory granules and might be some kind of complex protein.

Adrenal Gland Neoplasms↗
PubMed1988

Eosinophilic globules in fibroadipose tissue adjacent to Kaposi's sarcoma.

Eosinophilic globules have been reported in the sarcomatous tissue of the cutaneous type of Kaposi's sarcoma. This report presents a case in which similar eosinophilic globules were found in uninvolved area of fibroadipose tissue adjacent to the sarcomatous tissue in a 30-year old African woman. The histochemical characteristics of these globules were identical to those found within the sarcomatous tissue. The origin of these globules is not known, but a possible explanation is offered.

Adipose Tissue↗
PubMed1988

Black thyroid associated with minocycline therapy: histochemical and ultrastructural studies on the brown pigment.

The brown pigment found in the black thyroid, associated with minocycline therapy, was studied histochemically and ultrastructurally. The brown pigment was Fontana-Masson silver-positive, and was negative for iron, for autofluorescence under uv illumination, for periodic acid Schiff (PAS) and for acid-fast iodine peroxidase (AFIP) lipofuscin, and was bleached with potassium permanganate. The light and electron microscopic studies suggested that the pigment resembled melanin. Although the mechanism of the pigment deposition is not clear, a possible explanation may be that the pigment is related to direct oxidative degradation products of minocycline.

Aged↗
PubMed1987

A staining method for easy detection of eosinophilic globules in Kaposi's sarcoma.

Eosinophilic globules provide an important clue for diagnosis of Kaposi's sarcoma (KS). These globules were stained with periodic acid Schiff (PAS), periodic acid Schiff reagent after diastase digestion, phosphotungstic acid hematoxylin (PTAH) and autofluorescence under ultraviolet illumination. By these methods, however, eosinophilic globules in KS could not be readily identified. Therefore, we developed a new procedure for rapid identification of eosinophilic globules in KS tissues.

Humans↗
PubMed1986

Rapid identification of Klebsiella pneumoniae in the lung tissue by fluorescence microscopy.

Klebsiella pneumoniae is stained weekly with hematoxylin and eosin, moderately with periodic acid Schiff (PAS), and strongly by the Warthin-Starry method. Staining by the Warthin-Starry method, however, takes more than one hour. Therefore, we developed a new procedure for rapid identification of K. pneumoniae in paraffin sections of the lung under a fluorescence microscope.

Animals↗
PubMed1986

A rapid silver staining method for identification of Mycobacterium leprae in histologic sections.

A few methods have been reported for the purpose of staining Mycobacterium leprae in paraffin sections, including Fite oil fuchsin method, auramine-rhodamine method, and Blanco-Fite silver method. Among these staining techniques, Fite oil fuchsin method and auramine-rhodamine method are popular. However, the Blanco-Fite silver method takes approximately 20 days. Therefore, we developed a new procedure for rapid identification of M. leprae in paraffin sections using another silver solution and found that the procedure gave stable and satisfactory results. This new method has proved superior to others in demonstrating a reliable staining for M. leprae.

Humans↗