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M Senba

Publications and source records attributed to M Senba.

71 records · Page 4Linked to original sources
PubMed1984

A reliable silver staining method for identification of Pneumocystis carinii in histologic sections.

The acquired immune deficiency syndrome (AIDS) is characterized by the development of Kaposi's sarcoma and several opportunistic infections including pneumonia caused by Pneumocystis carinii. As a staining method for Pneumocystis carinii, the methenamine-silver nitrate method has been used routinely. Most of fungi can be stained easily by this technique but the identification of Pneumocystis carinii is not always easy. The author has improved the method by using ammoniacal silver nitrate and found that this was a reliable staining method for Pneumocystis carinii. Moreover, this method proved to be superior to others in demonstrating Pneumocystis carinii in histologic sections.

Aged↗
PubMed1982

Staining method for hepatitis B surface antigen (HBs Ag) and its mechanism.

HBs Ag in the hepatocytes has been stained by the orcein method. As degenerative and necrotic cytoplasms of hepatocytes are occasionally stained also by this method, it is difficult to identify HBs Ag. The orcein dye has different staining properties from lot to lot, causing difficulty in obtaining stable results. Therefore, the author tried to modify the orcein method and found that the procedure using a sensitizer (ferric ammonium sulfate or uranium nitrate) after oxidizing the solution gave stable and satisfactory results. By this method, in the hepatocytes, only HBs Ag is stained positively. The mechanism of function of the sensitizer is not clear, but it is considered that ferric or uranium ions occupy small gaps in the protein molecular structure in the cells before orcein molecules occupy these gaps and that the method does not give any false positive result. It is also suggested that various HBs Ag staining dyes can react with sulfonic acid residue groups which are formed by the oxidization of disulfide group of proteins. Another new staining method for HBs Ag using resorcin fuchsin has been developed. The results of this method were also discussed.

Hepatitis B Surface Antigens↗
PubMed1980

Pluripotent hemopoietic precursors in vitro (CFUMIX) in aplastic anemia.

When human marrow cells were cultured in a medium containing alpha-medium, methylcellulose, fetal calf serum, bovine serum albumin, erythropoietin, and leucocyte-conditioned medium, mixed colonies composed of erythrocytic cells and granulocytes were formed. The clonal nature of the mixed colonies was confirmed by the linear relationship between the numbers of cells plated and the number of colonies, and the absence or presence of Y-chromatin in the mixed colonies in a co-culture experiment with male and female cells. Using the methylcellulose cell culture techniques, the pluripotent hemopoietic precursors (CFUMIX) in marrow cells from 15 patients with aplastic anemia were assayed. In the control subjects of patients with iron-deficiency anemia, lymphoadenitis, reactive leucocytosis or Hodgkin's disease, 8 X 10(5) marrow cells in 4 dishes produced 12.7 +/- 6.9 (mean +/- SD) mixed colonies. On the other hand, 8 X 10(5) marrow cells from patients with aplastic anemia formed only 2.1 +/- 5.5 (mean +/- SD) mixed colonies. Furthermore, the marrow cells from 5 patients who were repeatedly receiving transfusions contained no CFUMIX which give rise to mixed colonies. The present results provided the first direct evidence that pancytopenia in most patients with aplastic anemia results from a reduced influx into the compartment of maturing hemopoietic cells from the compartment of pluripotent hemopoietic precursors.

Adult↗
PubMedDate not supplied

Long-term trend of decreasing frequency of hepatitis B virus infection.

Statistical and histopathological analysis was carried out to determine whether hepatitis B surface antigen (HBsAg) with the autopsy diagnosis of hepatitis, cirrhosis, and hepatocellular carcinoma declined during 1964-1991. In this study, the liver specimens from 673 autopsy cases at Nagasaki University Hospital were used, and these materials were stained for HBsAg with immunoperoxidase method. For statistical calculation, we used Cochran's chi-square test with one degree of freedom for linear trend for proportions. We investigated HBsAg positive rates for the four time periods, 1964-1970, 1971-1977, 1978-1984, and 1985-1991, and for the diagnosis of hepatitis, cirrhosis, and hepatocellular carcinoma. The decreasing linear trend of HBsAg positivity with time was significant for hepatocellular carcinoma and cirrhosis.

Carcinoma, Hepatocellular↗