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Biomedical subjects

M Shiratsuchi

Publications and source records attributed to M Shiratsuchi.

24 records · Page 2Linked to original sources
PubMed1988

Proteose peptone enhances production of tissue-type plasminogen activator from human diploid fibroblasts.

Proteose peptone markedly enhanced the production of human tissue-type plasminogen activator (t-PA) from confluently cultured cell of human diploid fibroblasts. The cells continued synthesizing and secreting high levels of t-PA under periodic replacement of medium containing proteose peptone for more than one month. The highly increased activity correlated with equally increased levels of t-PA antigen and concomitantly increased levels of t-PA specific mRNA.

Caseins↗
PubMed1988

Isolation and identification of prostaglandin I 2 stimulating factor from human plasma.

To isolate and identify the plasma factor which stimulates prostaglandin I 2 production by rat aortic ring, a human plasma fraction which showed a major stimulating activity on prostaglandin I 2 production was purified by ultrafiltrate, Sephadex G-10 gel filtration and QAE-Sephadex column chromatography. The purified plasma factor was identified as uric acid by its ultraviolet and infrared absorption spectroscopy, and 1H nmr and 13C nmr spectroscopy. The stimulating activity of the purified plasma factor and that of authentic uric acid coincided with each other. The stimulating potency of uric acid at its physiological concentration in human plasma (about 50 micrograms/ml) was half of the deproteinized human plasma, and was about 30 fold stronger than that of L-tryptophan, a cofactor of prostaglandin hydroperoxidase.

Adult↗