Biomedical subjects

M Shirtliff

Publications and source records attributed to M Shirtliff.

3 recordsLinked to original sources

PubMed2005

Can laboratory reference strains mirror "real-world" pathogenesis?

The extraordinary plasticity of bacterial genomes raises concerns about the adequacy of laboratory-adapted reference strains for the study of "real-world" pathogenesis. Some laboratory strains have been sub-cultured for decades since their first isolation and might have lost important pathophysiological characteristics. Evidence is presented that bacteria rapidly adapt to in vitro conditions. Genomic differences between laboratory reference strains and corresponding low-passage clinical isolates are reviewed. It appears that no bacterial strain can truly represent its species. For DNA microarray and proteomic studies, this limitation might be overcome by the summation of individual genomes to produce a species-specific virtual supragenome.

Escherichia coli K12↗

PubMed1999

The host and the skeletal infection: classification and pathogenesis of acute bacterial bone and joint sepsis.

Bone and joints are normally sterile areas. Bacteria may reach these sites by either haematogenous spread or spread from an exogenous or endogenous contiguous focus of infection. Bone infection, or osteomyelitis, is characterized by a progressive infectious process resulting in inflammatory destruction of bone, bone necrosis and new bone formation. Joint infections, or infectious arthritis, arise either from the haematogenous spread of organisms through the highly vascularized synovial membrane or from direct extension of a contiguous bone or soft tissue infection. The most commonly involved joints are the knee and the hip, although any joint can become infected. Infectious arthritis is monoarticular in 90% of cases. Some of the questions to be answered in this chapter include: how bacteria reach and cause damage in the bones and joints; what the current classification systems of bone and joint infections are; what some risk factors and host factors associated with bone and joint infection are; what some current characteristics of musculoskeletal infections are and whether the damage to joints can be diminished by treatment.

Acute Disease↗

PubMed1997

Staging and staging application in osteomyelitis.

Osteomyelitis is traditionally staged by the Waldvogel classification system. The Waldvogel classification is an etiologic system and does not readily lend itself to guiding surgical or antibiotic therapy. Other classifications have been developed to emphasize different clinical aspects of osteomyelitis. These classifications include those of Ger, Kelly, Weiland, Gordon, May, and Cierny-Mader. The Cierny-Mader classification is based on the anatomy of bone infection and the physiology of the host. The Cierny-Mader classification permits the development of comprehensive treatment guidelines for each stage. The Cierny-Mader classification is used to demonstrate the application of staging for the diagnosis and treatment of osteomyelitis.

Anti-Bacterial Agents↗