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Biomedical subjects

M Shishido

Publications and source records attributed to M Shishido.

At least 19 recordsLinked to original sources
PubMed1995

Use of species- and strain-specific PCR primers for identification of conifer root-associated Bacillus spp.

A polymerase chain reaction amplification of 23S rDNA was developed to identify Bacillus spp. recovered from roots, mycorrhizae, and rhizosphere soil of conifers. The polymerase chain reaction incorporated a conserved 23S rDNA forward primer in combination with a reverse primer designed to hybridize exclusively to nucleotide sequences of either B. polymyxa or B. mycoides. The amplification provided a rapid and simple means of identifying DNA from isolates of Bacillus, and could be used directly on whole Bacillus cells or mixed populations. The reaction was used to detect and differentiate these Gram-positive species from agar plates inoculated with samples from various conifer samples. A strain-specific primer was also synthesized and used to identify Bacillus which were established within conifer roots 4 weeks after inoculation.

Bacillus↗
PubMed1995

A case of pretibial myxedema associated with Graves' disease: an immunohistochemical study of serum-derived hyaluronan-associated protein.

The myxomatous materials in cutis from a patient with pretibial myxedema (PTM) with Graves' disease were found to be mainly hyaluronic acid (HA), which had accumulated extensively in the upper dermis. Fibroblasts were increased in number in the mid to lower dermis. To clarify the mechanism of the deposition of HA in the dermis, we employed an antibody against serum-derived hyaluronan-associated protein (SHAP). This immunohistochemical study disclosed that the greater part of the fibroblasts in the mid to lower dermis stained positively, while the fibroblasts surrounded by fairly large amounts of HA in the upper dermis stained faintly or not at all. From these results, we suspect that the accumulation of HA progresses from the upper to the low dermis and that the interaction of fibroblasts and SHAP leads to development of the physiophathological cutaneous changes seen in our case.

Fibroblasts↗
PubMed1995

[Two cases of CA19-9 producing pulmonary papillary adenocarcinoma].

Two cases of CA 19-9 producing pulmonary papillary adenocarcinoma were reported. Pathological examination showed a well differentiated papillary adenocarcinoma with partially bronchiolo-alveolar cell type. Immunohistochemical study showed a positive straining for CA 19-9 on tumor cells. The level of CA 19-9 decreased after lobectomy and anti-cancer chemotherapy using CDDP and VDS. The series of CA 19-9 level is very useful to evaluate the effect of surgery and chemotherapy. Also it is useful to find out the recurrence of carcinoma.

Adenocarcinoma, Papillary↗
PubMed1995

[Treatment of tuberculous tracheobronchial stenoses with expandable metallic stent (EMS)s placed during percutaneous cardiopulmonary support (PCPS)].

A 63-year-old woman was taken to a hospital by ambulance because of dyspnea. She was admitted in nearly complete cardiopulmonary arrest. CPR was started immediately, and the patient was mechanically ventilated via a tracheostomy tube. Abnormally high airway pressures 40-50 cmH2O were required during positive pressure ventilation. Saber-sheath like stenoses of the lower trachea 3 cm long and left main stem bronchus 2 cm long were found by bronchoscopy. It was difficult to pass a 3-mm-diameter bronchoscope through these stenoses. The right main stem bronchus was completely obstructed. A chest radiograph showed a completely collapsed right lung and a scarred upper lobe of the left lung, probably caused by previous tuberculosis. Weaning from the ventilator was attempted over three months. However, the patient could not breathe by herself because of airway obstruction. She was referred to our hospital for the placement of stents into the stenotic parts of the airways. A longitudinally connected Gianturcotype EMS, 15 mm in diameter and 50 mm long was placed in each area of stenosis under general anesthesia. During the placement of EMSs, PCPS was used for 113 minutes instead of mechanical ventilation of the lungs. Hemodynamics were stable during PCPS. A bypass flow of 2.0-2.8 l/min/m2 was used. Oxygen saturation of arterial blood taken from an earlobe was maintained at 100%. Blood taken from the left radial artery had a PaO2 of 269.4 mmHg and a PaCO2 of 40.2 mmHg. There were no complications after the procedure. One month later, the stenoses had become slightly dilated.(ABSTRACT TRUNCATED AT 250 WORDS)

Bronchial Diseases↗
PubMed1995

[A case of inflammatory bronchial polyp under treatment of tuberculosis].

A 76-year-old man was admitted to our hospital because of pulmonary tuberculosis. He was treated by antituberculosis drugs. Four months later, bronchoscopic examination revealed three polypoid tumors. One of them was a small polypoid lesion with the pus containing acid-fast bacilli. The others were smooth surfaced black polypoid tumors without pus. Although biopsy specimens from these tumors showed a non-specific inflammatory change, the former could be considered as a tuberculous change. Four months later, the polypoid tumors disappeared and anthracosis was found at the site of black polypoid tumors. It is suggested that a polypoid tumor with tuberculous findings was in the earlier stage than the other tumors during the healing process of bronchial tuberculosis.

Aged↗
PubMed1994

[A case of idiopathic pulmonary fibrosis associated with bilateral pulmonary arterial thrombosis found at autopsy].

A 73-year-old woman admitted to the hospital with dyspnea on exertion. Chest radiography revealed a diffuse interstitial shadow; PaO2 was 72 Torr, and PaCO2 was 41 Torr. Laboratory examination results were compatible with idiopathic pulmonary fibrosis (IPF). Prednisolone relieved the dyspnea, but tapering of the drug led to a recurrence of this symptom. Pulse therapy was started and azathioprine was added to the corticosteroid. Over the course of 6 months of treatment, the patient's respiratory function remained fairly stable. Then respiratory distress was induced by an attack of atrial fibrillation, with relief provided by anti-arrhythmic drugs and large doses of corticosteroids. The patient died suddenly 3 weeks later. An autopsy revealed large thrombi in both pulmonary arteries with 90% stenosis. Parts of the thrombi were organized, which suggests that 2-3 weeks had elapsed since initial thrombus formation. Histological examination of lung tissue showed usual interstitial pneumonia. Pulmonary thromboembolism should be considered in patients with IPF if respiratory distress suddenly and unexpectedly worsens.

Aged↗
PubMed1993

[Evaluation of CEA, SLX and CA125 in active pulmonary tuberculosis].

Serum tumor markers (CEA, SLX, CA125) were evaluated in 123 patients with active pulmonary tuberculosis. The results were as follows: Elevated serum CEA, SLX and CA125 levels were found in 16.9%, 39.5% and 44.4%, respectively, of patients with active pulmonary tuberculosis. Markedly elevated serum CA125 levels were found in some cases. On chest X-ray films, the factors associated with elevation of serum tumor marker levels were examined. Serum CEA and SLX levels were correlated with the extent of disease. Pleural effusion had no influence on serum tumor marker levels. Serum SLX levels were higher in patients without a tuberculous cavity than in those with a tuberculous cavity. After therapy with antituberculous drugs, the mean values of serum SLX and CA125 levels significantly decreased. The decrease in mean value of serum CEA levels was not significant, but in 4 of 8 cases, elevated serum CEA levels were normalized after therapy. On the other hand, two cases with increased serum CEA or SLX level died of respiratory failure. These results suggest that to follow up patients with elevated serum tumor markers is useful for the evaluation of disease activity of pulmonary tuberculosis. Further elevation of serum tumor marker levels after antituberculous therapy may indicate the presence of cancer or deterioration of the disease.

Adolescent↗
PubMed1993

[A case of endobronchial chondroma in a patient with atypical mycobacterial infection].

A case of endobronchial chondroma in a 74-year-old man with atypical mycobacterial infection is reported. Bronchoscopy was performed in order to exclude the possibility of malignancy. At bronchoscopic examination, a hemispherical polypoid tumor with smooth surface was found at the orifice of left B3 and was removed by transbronchial biopsy forceps. Histologically the tumor was composed of cartilage tissue. About 1 year after forceps resection, no growth of the tumor was seen in the left upper lobe bronchus on bronchoscopic examination. Endobronchial chondroma is relatively rare. We discuss the clinical features of 19 cases of endobronchial chondroma so far reported in Japan, including our case.

Aged↗
PubMed1993

[Three cases of lung cancer with calcification--demonstration by chest CT].

We report three cases of lung cancer with calcification shown on chest CT scans. Case 1 was a 63-year-old man with well differentiated squamous cell carcinoma of the lung. Chest CT scan showed pronounced central calcification within the tumor. Case 2 was a 57-year-old woman with small cell carcinoma of the lung. Chest CT scan showed diffuse amorphous calcification within the tumor. Case 3 was a 70-year-old man with lung cancer for which pathologic data was not available. Chest CT scan showed punctate and linear calcification of the cavity wall within the tumor. Microscopic examinations revealed dystrophic calcification in cases 1 and 2. Calcification has long been recognized as a distinguishing radiologic feature of a benign pulmonary mass, however, the presence of calcification within a tumor on chest CT scan does not exclude the diagnosis of lung cancer.

Aged↗
PubMed1992

[Two cases of adult onset of Still's disease in the elderly].

Two cases of adult onset of Still's disease is in a 83-year-old and a 61-year-old women. Both cases complained of high fever, arthralgia, sore throat and maculae. Examinations on admission revealed leukocytosis and negative antinuclear antibody. Administration of prednisolone resulted in improvement of the clinical symptoms and laboratory data. In general, the onset of this disease is usually in early adulthood, but in these two cases the onset was at an advanced age. These results suggest that Still's disease of adult onset should be included in the differential diagnosis of fever of unknown origin, if the patient is elderly. In the treatment of the disease, proper attention to side effects of prednisolone and complications can be important.

Aged↗
PubMed1992

[A case of idiopathic pulmonary fibrosis with histology of usual interstitial pneumonia that responded to pulse therapy followed by combined immunosuppression with prednisolone and azathioprine].

A 64-year-old woman who was admitted with cough and dyspnea showed severe hypoxemia and interstitial lung shadows. The clinical diagnosis was idiopathic interstitial pneumonia (synonymous with idiopathic pulmonary fibrosis in the United States), since there were no specific immunological or bacteriological findings. No clinical signs or laboratory data compatible with collagen disease were observed. Methylprednisolone pulse therapy was given followed by prednisolone (0.8 mg/kg) and azathioprine (15 mg/kg). Marked improvement of hypoxia, chest X-ray and spirometry results was observed after five weeks. Histological examination of an cases of residual interstitial shadow obtained by open lung biopsy revealed usual interstitial pneumonia. Tapering of the immunosuppressant drugs led to a recurrence 3 months later, which was controlled by reintroduction of the same regimen. Therefore, only prednisolone was tapered, and data obtained in an outpatient clinic 6 months after the recurrence were as follows: %VC 108%, %DLco 72%, PaO2 80 Torr. The value of this regimen for acute IPF or exacerbation of IPF is suggested because of its life-saving effects.

Aged↗
PubMed1992

[Immunological reactions in patients with pulmonary aspergilloma].

A 52-year-old man with pulmonary aspergilloma is reported. In immunoassays, type I, III and IV hypersensitivity reactions of Gell & Coombs were observed. The finding of such immunological reactions, especially type I hypersensitivity, has rarely been observed in this disease. The present study was performed to investigate these reactions, especially to determine whether type I allergy participates in pulmonary aspergilloma. We reviewed 126 cases of pulmonary aspergilloma reported in the Japanese literature between January 1980 and April 1991. The following results were obtained: Elevation of serum IgE was observed in 49% of the subjects examined, positive IgE RAST score in 28%, precipitating antibody against A. fumigatus in 79%, and positive lymphocyte stimulation test using A. fumigatus antigen in 68%. In conclusion, type I, III and IV hypersensitivity of Gell & Coombs appears to participate in pulmonary aspergilloma. The occurrence of type I hypersensitivity is not rare in this disease.

Aspergillosis, Allergic Bronchopulmonary↗
PubMed1984

[A case of giant cell tumor of the sphenoid bone--special emphasis on its clinico-radiological features and radiosensitivity].

A 19-year-old man was admitted to the hospital because of blurred vision, visual field defect, diplopia and hypesthesia of the left face. Neurological examination on admission revealed impairments of the II, III, IV, VI cranial nerves bilaterally and the first branch of the V nerve on the left. X-ray films of the skull showed a marked decalcification of the sella and upper portion of the clivus. Cerebral angiography demonstrated a moderately vascularized, large tumor in the sella-clival region. The tumor was supplied mainly by the branches of the right internal carotid artery, which was occluded at the cavernous portion. CT scans showed a large, oval mass located at the mid-portion of the anterior and middle fossa. Hounsfield number of the tumor was approximately 64.0, but several high density spots, probably due to destroyed bone fragments, were seen inside. The tumor was markedly enhanced with contrast medium. Three successive craniotomies were carried out through right fronto-temporal approaches, but total removal of the tumor was not achieved. Histological examination of surgical specimens disclosed that the tumor was consisted of abundant multinucleated giant cells and fewer spindle shaped stromal cells. Postoperative radiotherapy by telecobalt was tried and a total dose of 70Gy was delivered to the residual tumor. Effect of radiotherapy was remarkable and the size of the tumor on CT was markedly reduced to the extent of 10% of the pre-radiation tumor size. The patient was discharged in a good condition and there have been no signs of recurrence for 10 months so far. On the basis of our case and cases reported in the literature so far, the authors discussed clinical and radiological features, difficulty in surgical treatment and radiosensitivity of giant cell tumor of the sphenoid bone.

Adult↗
PubMed1983

[Chronic subdural hematoma associated with arachnoid cyst--study of the mechanism of its development].

A significant number of cases of chronic subdural hematoma associated with middle fossa arachnoid cyst has been reported in literature, but sufficiently tenable explanation for co-occurrence of both lesions has not yet proposed. In this study, authors try to elucidate mechanisms involved in development of chronic subdural hematoma and arachnoid cyst in the same patient. Eighteen cases with arachnoid cyst in the middle fossa were diagnosed by CT scan during last 5 years in our institute. Among these, five patients had chronic subdural hematoma additionally to their middle fossa arachnoid cyst. Analysis of clinical, roentgenological data and operative findings in our five cases and reviewing of cases reported so far in the literature makes clear the following characteristics in this pathological condition. 1) Patients of chronic subdural hematoma associated with arachnoid cyst were obviously younger than patients with usual chronic subdural hematoma. 2) Chronic subdural hematoma developed in the same side to the associated arachnoid cyst. 3) Characteristic changes in the skull on x-ray films indicated the long lasting existence of middle fossa arachnoid cyst. On the other hand, history of cases suggested that chronic subdural hematomas had developed within recent 1-3 months. 4) Intracranial pressure tended to remain normal or slightly elevate. 5) Abnormal, small veins which run on the surface of the membranous capsule of arachnoid cyst and bridge the Sylvian fissure were not infrequently found at operation. These veins were not able to visualized on routine angiography. On the basis of these clinical and pathological characteristics, authors infer a mechanism for development of subdural hematoma associated with arachnoid cyst. The presence of middle fossa arachnoid cyst must increase a compressibility of the intracranial content, especially of the ipsilateral cerebral hemisphere and it predisposes for development of chronic subdural hematoma.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗