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M Sigfrido Rangel-Frausto

Publications and source records attributed to M Sigfrido Rangel-Frausto.

6 recordsLinked to original sources
PubMedDate not supplied

Response of IFN-gamma and IgG to ESAT-6 and 38 kDa recombinant proteins and their peptides from Mycobacterium tuberculosis in tuberculosis patients and asymptomatic household contacts may indicate possible early-stage infection in the latter.

BACKGROUND: The ESAT-6 antigen from Mycobacterium tuberculosis evokes a protective immune response in murine models and is widely recognized by tuberculosis patients (TB) and healthy household contacts (HHC). However, little is known about human immune response to this antigen in populations from areas of high endemicity. This study aimed to determine the capacity of T-cells from a group of TB patients and HHC for cell proliferation and production of cytokines type Th1 or Th2 (IL-4, IL-10, and IFN-gamma) and to identify total IgG reactivity to the recombinant protein rESAT-6 and five overlapping synthetic peptides as well as to r38 kDa and two peptides. METHODS: T-cells from nine TB patients and nine HHC were stimulated with rESAT-6 and five overlapping synthetic peptides, previously selected from a set of 21 peptides and each of 16 amino acids in length (P1, P4, P6, P8, and P20). Similar experiments were carried out with r38 kDa and two peptides of 20 amino acids in length (38G and 38K). Cytokines in supernatants and total IgG from serum were determined by ELISA. RESULTS: Stimulation index (SI) was highest in HHC to rESAT-6 and peptides P1, P8, and P20. Differences in response to 38 kDa and 38G peptide between TB patients and HHC were not demonstrated. Cytokines from T-cell cultures were tested with a resulting SI=3.0. IFN-gamma was produced predominantly in HHC to rESAT-6, P8, and P20, while in TB patients production of IL-10 was detected in relation to r38 kDa. IL-4 was detected in minimal amounts in both groups. IgG from TB patients was predominantly recognized in connection with rESAT-6 and the P4 peptide, with an important response against r38 kDa detected in HHC. CONCLUSIONS: ESAT-6 recognition by HHC could indicate that these responses represent possible early-stage infections.

Adult↗
PubMedDate not supplied

Sepsis: still going strong.

Sepsis definitions help to understand and to better define a group of syndromes secondary to an infectious insult. The hierarchical continuum of inflammatory response leads, in absence of counterregulatory forces, to organ damage and death. We have learned first the response to treatment and afterwards the pathophysiology behind it. This lesson has, of course, not always been followed by a reduction of mortality. The definition, natural history, risk factors, diagnoses, and treatment based on emerging evidence will help to improve patient outcomes and mortality. Standardized care seems to improve survival, and validation and further evaluation of this care is necessary to maximize resources and outcomes.

Hospital Mortality↗
PubMedDate not supplied

[Transmissible spongiform encephalopathies].

Transmissible spongiform encephalopathies (TSE) are a group of diseases which have received a lot of attention in recent years. The interest on these diseases has been stimulated by the appearance of bovine spongiform encephalopathy (BSE) and the new variant of Creutzfeldt-Jakob disease (nvCJD); the latter is likely to be acquired by ingesting contaminated beef. Until now 109 cases of nvCJD have been reported, most of them occurring in the United Kingdom. Some experts think that this is the beginning of a nvCJD pandemic. Deep knowledge of the mechanisms of transmission of TSE is needed to prevent the emergence of a TSE pandemic in humans. We address various aspects of TSE and discuss prevention methods of TSE in ruminants and humans.

Animals↗
PubMedDate not supplied

[Nosocomial infection surveillance: experience at a cardiology hospital in Mexico].

OBJECTIVE: To describe the results of the Nosocomial Infection surveillance program at the Cardiology Hospital in Centro Médico Nacional Siglo XXI of the Mexican Social Security Institute. METHODOLOGY: To inform of the epidemiologic follow-up results from January 2000 to July 2003. Global frequency, infection rates by infection site, hospital services, and frequency of most common microorganisms were estimated. RESULTS: During this period, global incidence showed that in every 100 discharges, there were 4.3 infections (4.3/100). Frequency of infection was slightly higher in the surgical intensive care unit. Average infection rate in lower respiratory tract infections was 27/1,000; in surgical-site infections: 8/1,000 (mediastinitis 0.8/1,000); it was found that in urinary tract infection, rate was 6.6/1,000. Most commonly isolated microorganisms were: coagulase-negative Staphylococcus (25%), Enterobacter sp. (17%), Candida albicans (13%), S. aureus (9%), P. aeruginosa (9%) and K. pneumoniae (6%). CONCLUSION: This study describes the epidemiology of nosocomial infections in a Cardiology Hospital. The information is obtained through epidemiologic follow-up programs. This information is very important to develop specific strategies for control of infections.

Cardiac Care Facilities↗
PubMedDate not supplied

[Antibiotic resistance of Helicobacter pylori in an infectious disease referral center].

OBJECTIVE: To estimate the frequency of H. pylori clinical isolates resistant to six commonly used antimicrobials. DESIGN: Cross-sectional observational study. SETTING: A tertiary-referral health care institution in Mexico City. PARTICIPANTS: 31 isolates of H. pylori from 31 patients with chronic antral gastritis were obtained from gastric mucosal biopsy specimens. MAIN OUTCOME MEASURE: The Minimum Inhibitory Concentration (MIC) to ampicillin, amoxycillin, tetracycline, doxycycline, metronidazole and to colloidal bismuth subcitrate was determined by the agar plate dilution test. RESULTS: All isolates showed to be susceptible to the former four antibiotics but only in 46% and 55% growth was inhibited by 8 micrograms/mL and 16 micrograms/mL of metronidazole, respectively. All isolates were inhibited by < or = 128 micrograms/mL of bismuth. A 50% increase in the percentage of metronidazole-resistant isolates (MIC > or = 8 micrograms/mL) between 1988 to 1992 was observed. CONCLUSION: There is a need of future studies in our setting aimed at assessing the cost/effectiveness of diverse H. pylori-associated peptic ulcer treatment options.

Amoxicillin↗