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Biomedical subjects

M Sikorski

Publications and source records attributed to M Sikorski.

At least 19 recordsLinked to original sources
PubMed2005

Dynamics of cervical langerhans cell counts in the course of HPV-positive CIN treatment with the use of human recombinant interferon gamma.

OBJECTIVES: Langerhans cells play a pivotal role as professional antigens presenting cells in cervical epithelium, thus changes in their density or/and function may profoundly influence the proper activation of the afferent arm of immune response in cases of HPV-related intraepithelial lesions. AIM OF THE STUDY: Assessment of intraepithelial Langerhans cell count changes in CIN I/CIN II after human recombinant interferon gamma (IFNgamma) application and correlation with clinical outcome. MATERIAL & METHODS: The present study is a part of prospective trial on IFNgamma application in the treatment of CIN I/CINII associated with high-risk HPV infection. Seventeen subjects underwent uniform IFNgamma treatment (four intracervical injections in a two-day interval for a total dose of 6,000,000 IU). Langerhans cells were stained within cervical punch biopsy specimens with the use of polyclonal anti-S-100 antibody according to the three-step indirect peroxidase protocol, and their mean count calculated for the following groups: before IFNgamma treatment launching, immediately after completion of the treatment, and after two months of follow-up. RESULTS: The analysis revealed a rapid and significant increase in Lagerhans' cell count immediately after treatment completion (21.17/mm2 and 25.94/mm2, respectively, at p = 0.019) which further increased in the group of complete response (in 9 subjects; 32.22/mm2). After transient elevation of the Langerhans' cell count it returned to a level even lower than initially in the non-responder group (4 subjects; 20.25/mm2). CONCLUSION: Our data strongly support the observation from static studies suggesting that regression of HPV-related cervical lesions is associated with increased density of epithelial Langerhans cells.

Adult↗
PubMed2004

Characterization of edible oils using total luminescence spectroscopy.

Total luminescence spectroscopy was used to characterise and differentiate edible oils and additionally, to control one of the major problems in the oil quality--the effect of thermal and photo-oxidation. We studied several vegetable oils available on the Polish market, including soybean, rapeseed, corn, sunflower, linseed and olive oils. Total luminescence spectroscopy measurements were performed using two different sample geometries: front-face for pure oil samples and right-angle for transparent samples, diluted in n-hexane. All the samples studied as n-hexane solutions exhibit an intense peak, which appears at 320 nm in emission and 290 nm in excitation, attributed to tocopherols. Some of the oils exhibit a second long-wavelength peak, appearing at 670 nm in emission and 405 nm in excitation, belonging to pigments of the chlorophyll group. Additional bands were present in the intermediate range of excitation and emission wavelengths; however, the compounds responsible for this emission were not identified. The front-face spectra for pure oils included chlorophyll peaks for most samples, and some additional peaks in the intermediate range, while the tocopherol peaks were comparatively less intense. The results presented demonstrate the capability of the total luminescence techniques to characterise and differentiate vegetable oil products, and additionally, to characterize the effect of thermal and photo-oxidation on such products. In the photo-oxidation experiments, special attention was paid to possible involvement of singlet oxygen. Experiments were done to monitor the highly specific O2(1delta(g)) --> O2(3sigma(g)-) singlet oxygen emission at 1270 nm. Thus, total luminescence spectroscopy presents an interesting alternative to time-consuming and expensive techniques such as gas or liquid chromatography, mass spectrometry and other methods requiring wet chemistry steps.

Luminescent Measurements↗
PubMed2003

Recombinant human interferon gamma in the treatment of cervical intraepithelial neoplasia (CIN) associated with human papillomavirus (HPV) infection.

Human papillomavirus (HPV) proteins E6 & E7 are considered to be the constitutively expressed neoantigens in a vast majority of cervical squamous intraepithelial lesions and cancers. Data available from in-vitro, animal, and small clinical trials suggest that the immunological properties of interferon gamma might enhance early viral protein presentation, thus stimulating a cytotoxic response. In order to study this effect in vivo we undertook a trial in which 20 women with a definite diagnosis of cervical intraepithelial neoplasia (CIN) grade I or II with coexistent high-risk HPV infection (detected by the Hybrid Capture System) underwent four months observation followed by intracervical administration of INFgamma in cases without spontaneous regression (17 cases). Human recombinant interferon gamma 1-b (Imukin) was administered intracervically four times in equal doses in two-day intervals to a total dose of 6,000,000 IU. The results of therapy were verified by punch biopsy evaluation and HPV-DNA testing two months after completion, and revealed a complete response in nine women (complete regression of CIN and remission of HPV infection in 53% of treated cases) and partial response in four cases (lower grade of CIN or/and remission of HPV infection--23.5%). The differences between spontaneous (before treatment) and treatment-related regressions were significant at p < 0.05. We conclude that in selected cases (mainly young women who have not completed their procreation and are compliant with the therapy) a conservative approach to CIN management with intracervical IFNgamma injections seems to be a valuable method.

Adult↗
PubMed2001

Photophysical properties of lumichromes in water.

The photophysics of lumichrome, 1-methyllumichrome, and lumiflavin in water solutions have been investigated. Fluorescence lifetimes of 2.7 and 2.2 ns were observed for lumichrome and 1-methyllumichrome, respectively, the corresponding triplet state lifetimes of 17 and 18 micros have been obtained from the transient absorption spectra. Evidence for long lived species with absorption maxima near 450 nm and lifetimes of ca. 400 micros has been found in the transient absorption spectra of both lumichromes. Quantum yields for the sensitised production of singlet oxygen, phi(Delta), are 0.36 and 0.41 for lumichrome and 1-methyllumichrome, respectively, in D(2)O.

Journal Article↗
PubMed1999

[Surgical approach in cases of adnexal pathology in adolescents and young women].

Retrospective analysis comprised 108 cases of adnexal pathology found at laparotomy in the group of adolescents and young women up to the age of 25. The most common condition necessitating the laparotomy was hemorrhagic corpus luteum (24% of cases). Adnexal cysts of different but benign histological structure had been found in 30.5% of cases. Malignant germ cell tumors (6 cases--dysgerminoma and teratoma immaturum) all of which at Ia stage at the time of laparotomy, had been treated conservatively with unilateral salpingo-oophorectomy. Authors suggest that the conservative approach should be applied in most cases of adolescents' adnexal pathology.

Adnexal Diseases↗
PubMed1999

[Endometriosis in adolescents and young women. Can we afford to delay the diagnosis?].

Retrospective analysis performed on medical records of 129 adolescents and young women treated surgically at I Dept. of Surgical Gynaecology during the nine years period (1989-1998) revealed 22 cases (17%) of endometriosis. Main indication for surgical intervention, apart from dysmenorrhoea and cyclic abdominal pain, had been the presence of endometriod cyst, as disclosed by ultrasound investigation (66%). Despite of negative result of imaging or clinical examination subjective complaints necessitated laparotomy in 27% of cases. According to American Fertility Society endometriosis classification, 17% subjects presented endometriosis in I stage, 17%--in stage II and remaining 66%--endometrioid cysts with stage III. Authors conclude, that in cases with persistent abdominal pain, not responding to conventional therapy, diagnostic laparoscopy/laparotomy in young women should not be delayed, unless endometriosis is suspected.

Adolescent↗
PubMed1997

["Cold turkey" detoxification of drug, narcotic and chemical addicts conducted at the Occupational Diseases and Toxicology Ward of the Polish Center of Occupational Medicine in Poznan in 1986-1996].

In this paper we have discussed the method of the "pure" detoxification (without the use of any psychotropics) of patients addicted to drugs, narcotics and chemicals treated at the Occupational Diseases & Toxicology Ward of the WCMP in Poznań. The material includes 152 cases of addicted patients who were treated in the Ward in the period from 1986 till 1996. Our research proved that the applied method is safe for the patients. We also found that addicts sharing hospital room with general medicine, not addicted patients, experience a positive effects in the process of treatment and resocialization.

Adaptation, Psychological↗
PubMed1997

[Use of the Digene Hybrid Capture System for identification of the human papillomavirus (HPV-Human Papillomavirus) in women with cervical dysplasia].

The study was performed to assess prevalence of HPV-DNA infections using the Hybrid Capture System (Vira Papl Vira Typ ). In a group of 228 female patients with Papanicolaou smear grade I and II, high oncogenic risk HPV infections were found in the 15.78% and low oncogenic risk--in 7.01% of the investigated patients. In a group of 324 women with diagnosed CIN, the high oncogenic HPV infection were found in 70.4% of them (CIN I/II-60%, CIN III-87.11%), and the low oncogenic risk HPV infections in 11.1%.

DNA, Viral↗
PubMed1996

[Disturbances of glucose tolerance in workers exposed to electromagnetic radiation].

The study group was composed of 50 workers exposed to electromagnetic radiation (radiowaves). Out of them 31 persons (62%), employed mostly in the risk zone, showed irregular glycaemia after oral administration of 75 g of glucose. At normal blood sugar before breakfast, the glycaemia level was high following administration of glucose and it did not return to starting values after 2 hours. After 30 min from glucose administration the level accounted for 155 mg%, after 60 min-180 mg%, after 90 min-153 mg% and after 120 min-124 mg%, on average. In 10 persons (32%) with glucose tolerance disturbances, disorders in bioelectric activity of the brain (abnormal EEG record) were observed.

Adult↗
PubMed1996

[Usefulness of clinical evaluation for the level of CA-125 antigen and ultrasonographic color Doppler examination in diagnosis of ovarian tumor].

The objective of our study was to evaluate the predictive value of serum CA-125 levels and transvaginal ultrasound with color Doppler flow mapping (RI) in patients with benign and malignant ovarian tumors. The retrospective analysis included 78 women in whom 92 tumors were detected. Eleven patients had ovarian carcinoma (a total of 16 tumors). Predictive values were compared for tumor morphology, lowest resistive index (RI) < 0.40 and CA-125 > 35 IU/ml. Sensitivity, specificity, positive and negative predictive values in differentiating malignancy were highest for RI (94%, 87% 87% and 94%, respectively). Morphological criteria or CA-125 levels alone had lower predictive accuracy. Combining of the correctly identified all of the malignancies. It is concluded that preoperative transvaginal ultrasound with color Doppler flow mapping is valuable tool in the assessment of adnexal tumors and can be of assistance in prediction of ovarian cancer.

Adolescent↗
PubMed1994

[Amniotic fluid index during labor in women with EPH gestosis].

Intrapartal cardiotocographic disturbances of fetal heart rate patterns, the way of delivery and the neonate state have been analyzed in the group of 61 women in labor with pregnancy complicated by EPH gestosis. The results have been compared with pre-labor values of amniotic fluid index (AFI) in that group. In the group of 30 women in labor with AFI < or = 5 cm the analysis revealed the higher prevalence of non stress test reactivity losses, severe variable decelerations, late decelerations, cesarean sections and lower birth weight in comparison with the group of 31 women in labor with AFI > 5 cm. The study revealed that pre-labor AFI value estimation is usefull in predicting possibilities of fetal compromise during labor in women with pregnancies complicated by EPH gestosis.

Adult↗
PubMed1994

[Personal observations concerning surgical treatment of urinary stress incontinence in women].

Own observations concerning surgical treatment of urinary stress incontinence based upon the clinical follow-up of 82 treated women are presented. Depending on the method of surgical treatment, patients with urinary stress incontinence have been divided into 3 groups: I group-(42 women), where retropubic colpo-uretero-pexy has been performed following the rules previously suggested by Tanagho, Sprogis et al., with 92.8% of positive result, II group-(34 women), where Mieszczerski or Fothergill type of surgery has been performed, with 88.2% of positive results, and III group-(6 women), where vaginal vault has been suspended to the musculo-fascial flap, with positive results in all treated patients. The obtained results suggest that in every case of urinary stress incontinence the conservative approach should be the primary strategy of treatment. Analysis of the results revealed that retropubic colpo-uretero-pexy was an effective surgical method of urinary stress incontinence treatment in women with no cystocele and with proper uterine statics. In patients with correct uterine statics but with coexisting cystocoele, even of a minor grade, retropubical kolpouretropexy should be joined with the surgery from a vaginal access.

Adult↗
PubMed1994

[Endometriosis in clinical materials from the years 1981-1990].

In the group of 213 women with diagnosed endometriosis, after the histological analysis of the samples obtained during surgical procedure the following factors have been determined: the character and frequency of complaints, prevalence of the disease depending on the age of patients, it's localisation, coexistence with the neoplasms of the genital tract, indications for the surgical treatment and it's type. Taking into account the difficulties in diagnosing endometriosis, it's occurrence in young women and the possibility of malignant transformation along with the coexistence with malignant neoplasms of genital tract, we are convinced that the surgical intervention should be undertaken in order to achieve the better cure rate as well as to improve the accuracy of diagnosis.

Adult↗
PubMed1994

[Beeswax pessary in conservative treatment of uterine prolapse].

The own method of uterine prolapse treatment with the use of beeswax pessary is presented. The described method is applied in cases where general health conditions do not allow to perform the surgery. The study revealed that the beeswax is a good material to make a vaginal pessary. It's great plasticity allows individual modelling in compliance with anatomical conditions of treated patient. That provides the proper stability of uterine position. The risk of possible urinary stress incontinence due to uterine reposition is also diminished.

Animals↗
PubMed1987

Lupin leghemoglobins during root nodule development.

Two yellow lupin leghemoglobins, Lb I and Lb II, were purified to homogeneity using the HPLC technique for final separation. Lb I and Lb II were identified by the N-terminal sequences and their reaction with antibodies against electrophoretically pure leghemoglobin. The third Lb species was detected by the combined method of isoelectrofocusing and PAGE of Lb I. It seems that Lb III represents a posttranslational modification of Lb I. Developmental changes in Lb multiple forms were examined using the Western blotting method. The content of leghemoglobin, first detectable approximately 3 weeks after infection, increased up to 6-7 weeks, and then it remained at the same level until 8-9 weeks after the infection. At the early stages of nodule formation Lb I prevailed over Lb II, while later Lb II became the predominant form. This suggests physiological role of particular forms and precise regulation of the expression of Lb genes.

Amino Acid Sequence↗