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M Sillis

Publications and source records attributed to M Sillis.

30 records · Page 2Linked to original sources
PubMed1992

The rapid serological diagnosis of infectious mononucleosis.

A total of 121 samples of serum collected from 101 patients was tested to determine the sensitivity and specificity of a commercial latex agglutination test for detecting infectious mononucleosis heterophile antibody, a commercial immunofluorescence test for detecting antibody to Epstein-Barr virus capsid antigen and a rapid enzyme immunoassay for detecting antibody to Epstein-Barr virus nuclear antigen. Although the Epstein-Barr virus capsid antigen IgM indirect immunofluorescence test proved to be the most sensitive, false-positive reactions were seen when samples collected from patients with cytomegalovirus, hepatitis A virus, parvovirus and leptospira infection were tested. False-positive reactions were also seen with samples containing rheumatoid factor.

Adolescent↗
PubMed1992

Clinical evaluation of enzyme immunoassay in rapid diagnosis of herpes simplex infections.

AIMS: To evaluate the performance of antigen detection by IDEIA (NovoNordisk Ltd) in the rapid diagnosis of potentially serious herpes simplex (HSV) infections. METHODS: Nine hundred and twelve specimens from a variety of clinical sites, including ocular, mucocutaneous, respiratory and genital material, urines and necropsy tissue, were compared by enzyme immunoassay (EIA) and conventional culture for the presence of HSV. RESULTS: The EIA performed to a high level of sensitivity and specificity using a variety of specimen types. Some problems were encountered using cervical swabs from pregnant women and necropsy brain tissue. Analysis of clinical and contact history data of most patients giving discrepant results supported the evidence of recent HSV infection obtained by EIA. The mean culture time was 2.4 days (range one to eight days). CONCLUSIONS: The HSV EIA test performed to a high level of sensitivity (93.7%) and specificity (96.6%) when compared with culture using a variety of clinical material. These results assumed cell culture was 100% sensitive and specific. The actual performance of the EIA test is probably much higher. This approach to rapid HSV diagnosis should be used more widely, particularly in potentially serious cases.

Antigens, Viral↗
PubMed1990

The limitations of IgM assays in the serological diagnosis of Mycoplasma pneumoniae infections.

The most useful and reliable serological investigations for the diagnosis of current Mycoplasma pneumoniae infection, including reinfection, were investigated. Paired sera and respiratory specimens from 115 patients with lower respiratory tract symptoms were examined for evidence of current M. pneumoniae infection by serological response, as measured by complement-fixation and indirect immunofluorescence tests for specific IgM, IgA and IgG, and also by culture of M. pneumoniae from respiratory material. Specific IgM was not always detectable in cases where other criteria indicated current or recent infection. On the basis of the present results, it is postulated that primary infection and reinfection may be differentiated by the presence or absence of specific IgM in the presence of elevated specific IgA levels and, therefore, that estimation of both IgM and IgA is necessary for the maximal detection of current M. pneumoniae infection, including reinfections. Specific IgG levels remained elevated for many weeks and were not useful diagnostically.

Agglutinins↗
PubMed1990

Evaluation of Serodia Myco II particle agglutination test for detecting Mycoplasma pneumoniae antibody: comparison with mu-capture ELISA and indirect immunofluorescence.

The Serodia Myco II particle agglutination test, which the manufacturers claim exclusively detects IgM antibody, was compared with two IgM-specific tests, a mu-capture ELISA, and indirect immunofluorescence for their ability to detect recent Mycoplasma pneumoniae infection. In general there was good agreement among the three tests, all three having similar sensitivity. One hundred and nine (78%) of serum samples gave concordant results in all three assays. Several sera gave positive particle agglutination titres, however, while being negative by the two other assays, and the Serodia Myco II test may not be as specific for detecting M pneumoniae IgM as the other two tests. While the Serodia Myco II test may be a good screening assay, it is unlikely to be a definitive test for M pneumoniae IgM, but may be better than the complement fixation test, particularly in younger patients in whom M pneumoniae IgM is found more frequently.

Agglutination Tests↗
PubMed1987

An investigation of the Mycoplasma pneumoniae infections in Cambridge in 1983 using mu-capture enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence (IF) and complement fixation (CF) tests.

The mu-capture, the ELISA, IF and CF tests were used to study Mycoplasma pneumoniae infections in Cambridge in 1983. We studied 393 samples of serum submitted for investigation of M. pneumoniae infection, from 298 patients. There was a good correlation between mu-capture ELISA and IF for detecting M. pneumoniae-specific IgM, and between elevated CF titer, and the presence of M. pneumoniae-specific IgM. There was also a clear correlation between M. pneumoniae-specific IgM status and age. M. pneumoniae-specific IgM and IgA were most often found together in sera, but in some sera, only one of these antibodies was found at significant levels. These findings were not age related, and thus not obviously useful in differentiating primary and recurrent M. pneumoniae infections.

Adolescent↗
PubMed1986

The clinical spectrum and diagnosis of Mycoplasma pneumoniae infection.

The clinical, radiographic and microbiological data of 47 patients with Mycoplasma pneumoniae infection admitted to three Norfolk hospitals during a 20-month period between 1982 and 1983 have been reviewed. Thirty-nine presented with pneumonia and eight with non-pulmonary infection. The M. pneumoniae specific IgM test was positive in 42 of 45 patients tested (89 per cent); in 39 the levels were diagnostic on admission. Cold agglutinins were detected in 27 (57 per cent) and a fourfold rise in complement fixation titre was demonstrated in 13 (29 per cent). Sputum culture was positive in 12 (26 per cent). The extrapulmonary manifestations observed were haemolytic anaemia (17 per cent), Stevens Johnson syndrome (4.1 per cent), neurological abnormalities (4.1 per cent), arthritis (2.1 per cent), hepatitis (2.1 per cent) and pericarditis (2.1 per cent). One patient with multilobe pneumonia, pericardial effusion and haemolytic anaemia died. Six patients presented with a history of illness longer than a month; in three the clinical and radiographic picture suggested chronic disease (pulmonary tuberculosis, lymphoma and unresolving pneumonia). There were no distinctive clinical or radiographic features of M. pneumoniae infection. Diagnosis, therefore, relies on serological tests of which the most useful is the rapid, specific IgM test, positive in 86 per cent of the admission sera.

Adolescent↗
PubMed1985

A micro-capture ELISA for detecting Mycoplasma pneumoniae IgM: comparison with indirect immunofluorescence and indirect ELISA.

A mu-capture ELISA was developed for detecting Mycoplasma pneumoniae-specific IgM, and compared with an indirect immunofluorescent antibody (IFA) technique and an indirect ELISA. mu-capture ELISA and IFA compared well and were found to be the most sensitive assays. The IFA test can be completed in 2 h whilst the results of the mu-capture ELISA can be available in 24 h. Both tests are amenable to routine diagnostic use and have similar sensitivity. Indirect ELISA was found to be less sensitive and less specific, giving high assay values with several sera having undetectable M. pneumoniae CF antibody or CF antibody in low titre. Serum samples obtained from 11 patients at various times after M. pneumoniae infection showed maximum antibody levels within the first month by all assays, with a gradual fall in amount of IgM with time when assayed by mu-capture ELISA, a more gradual decline by IFA and hardly any decline with indirect ELISA. It was concluded that the indirect ELISA is unsuitable for the investigation of possible M. pneumoniae infection because the sustained high assay values with serum samples taken many months after infection, make interpretation of the test results very difficult.

Antibodies, Bacterial↗
PubMedDate not supplied

Diagnosis of urethritis: role of polymorphonuclear leukocyte counts in gram-stained urethral smears.

Polymorphonuclear leukocytes (PMNs) in the gram-stained urethral smears of 236 consecutive sexually active men without gonorrhea were analyzed quantitatively. The frequency distribution of the highest count of PMNs per high-power field (hpf) showed a count of four PMNs to be the "cut-off" point separating men with urethritis from those without urethritis. This cut-off point correlated well with the presence of Chlamydia trachomatis as well as with turbid urine. However, the PMN count in the gram-stained urethral smear was found to be more sensitive than the appearance of the urine in the diagnosis of urethritis among those with minimal symptoms and signs and not harboring C. trachomatis. This study also demonstrated a close similarity as regards clinical features and PMN count in gram-stained urethral smears between those harboring Ureaplasma urealyticum and those with no organisms.

Adult↗