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M Simonneau

Publications and source records attributed to M Simonneau.

59 records · Page 4Linked to original sources
PubMedDate not supplied

Both upstream and intragenic sequences of the human neurofilament light gene direct expression of lacZ in neurons of transgenic mouse embryos.

Initial expression of the neurofilament light gene coincides with the appearance of postmitotic neurons. To investigate the molecular mechanisms involved in neuron-specific gene expression during embryogenesis, we generated transgenic mice carrying various regions of the human neurofilament light gene (hNF-L) fused to the lacZ reporter gene. We found that 2.3 or 0.3 kb of the hNF-L promoter region directs expression of lacZ in neurons of transgenic embryos. Addition of 1.8 kb hNF-L intragenic sequences (IS) enlarges the neuronal pattern of transgene expression. The 2.3-kb hNF-L promote lacZ-IS construct contains all regulatory elements essential for both spatial and temporal expression of the hNF-L gene during embryogenesis and in the adult. The use of a heterologous promoter demonstrated that the 1.8-kb hNF-L intragenic sequences are sufficient to direct the expression of lacZ in a NF-L-specific manner both temporally and spatially during development and in the adult. We conclude that these hNF-L intragenic sequences contain cis-acting DNA regulatory elements that specify neuronal expression. Taken together, these results show that the neurofilament light gene contains separate upstream and intragenic elements, each of which directs lacZ expression in embryonic neurons.

Animals↗
PubMedDate not supplied

[Anti-I anti-erythrocyte auto-immunization in malaria].

An anti-erythrocyte autoimmunization with anti-I specificity was shown in patients previously exposed to malaria (falciparum malaria in 98.7% of cases). An anti-I antibody was eluted from red cells in 14.6% of 41 patients with an acute P. falciparum infection. The frequency of the anti-I immunisation was found to be correlated to the rate of the antimalarial antibodies among the 152 patients without parasitaemia, coming from areas endemic for falciparum malaria: this frequency was 5.2% for a rate below 1/64, 9.7% for a rate between 1/64 and 1/256, and 34% for a rate above 1/1,024. Anti-I antibodies were more often noted in African patients coming from forest areas, considered as chronically infected with P. falciparum, and in other patients who have been in France for less than a month. No anti-I antibodies were found in the control group, composed of 52 Caucasian healthy subjects with no previous exposure to malaria. This anti-I immunisation was transitory and its disappearance was usually accompanied by a lowering of anti-malarial antibodies.

Africa, Northern↗