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M Simonsen

Publications and source records attributed to M Simonsen.

At least 55 records · Page 3Linked to original sources
PubMed1980

Linkage disequilibrium of MHC genes in the chicken. I. The B-F and B-G loci.

Two loci of the chicken MHC (the B complex) are expressed in erythrocytes, B-F and B-G. The former is homologous to the murine K and D loci and is also expressed in all white blood cells, while the latter is apparently restricted to RBC and is of unknown relationship to H-2 and HLA loci. A recombinant between two congenic, MHC-different strains, CB and CC, has permitted the production of antisera specific for the B-F and B-G alleles of these two strains, and these and other antisera have been used for typing of outbred populations of various chicken breeds. The cross-reactions found with haplotypes other than the donors' are extensive, sometimes even extreme, but it is possible to narrow the specificity of the typing sera by appropriate absorptions. With absorbed sera we have found a linkage disequilibrium which is almost certainly even stronger than that reported in studies of mammalian MHC loci. We hve also made observations which suggest that the gametic association of a given set of B-F and B-G alleles is probably not merely a matter of random crossing-over events.

Absorption↗
PubMed1979

Elution of chicken Ig from fixed target cells.

A method is described for preparing fixed chicken peripheral blood cells (PBC) for absorption of allo-antibodies and their subsequent elution. Glutaraldehyde fixation was satisfactory, and with 8 M urea elution haemagglutinating activity was preserved.

Adsorption↗
PubMed1978

[Basic immunology].

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Allergy and Immunology↗
PubMed1976

Allo-aggression in chickens. II. Cellular expression of the AA gene products.

B1-anti-B2 and B2-anti-B1 allo-antisera were raised by i.v. immunization of homozygous B1/1 and B2/2 chickens, respectively, with B1/2 cells of the following sources: peripheral blood leucocytes (PBL), erythrocytes, thymus cells and bursa cells. Purified suspensions of these all produced anti-B allo-antibodies which could both haemagglutinate red blood cells and strongly inhibit the GVH reactivity of blood lymphocytes of the original donor type. Absorption studies of the sera using cells of the same four sources showed that they could all completely remove the GVH inhibitory antibodies. A fact of particular importance is that B-haplotype determined antigens which are common to erythrocytes and lymphocytes seem to include the gene products of the allo-aggression locus, as earlier defined (Simonsen 1975).

Animals↗