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Biomedical subjects

M Sindjic

Publications and source records attributed to M Sindjic.

7 recordsLinked to original sources
PubMed1993

Laminin, fibronectin, and Goodpasture antigen detection in patients with Alport's syndrome.

Indirect immunofluorescence study with laminin and fibronectin monoclonal antibodies on paraffin sections, as well as with serum from a patient with Goodpasture's syndrome with high titer of autoantibodies that recognize the antigenic determinants in human glomerular and tubular basement membrane, was performed on 14 patients with Alport's syndrome and 5 specimens of normal renal tissue obtained from donors in cases of renal transplantation (control group). We found no binding of Goodpasture antigen to glomerular and distal tubular basement membranes in renal biopsy tissue from all 14 patients with Alport's syndrome. In contrast, there was bright linear fluorescence of Goodpasture antigen on glomerular and tubular basement membranes of normal renal material. There was no difference in laminin and fibronectin binding in patients with Alport's syndrome and controls. In all the cases binding was strongly positive. These results suggest an abnormality or absence of immunoreactive autoantigen in the glomerular and distal tubular basement membrane in patients with Alport's syndrome. Therefore, Goodpasture antigen detection could be an important diagnostic method in early stages of Alport's syndrome when characteristic morphological changes are not yet developed.

Adolescent↗
PubMed1993

Spontaneous blastogenesis profiles and IL-2 receptor expression on kidney allograft.

To examine immune activation rate of interstitial and glomeruli infiltrating MNC in different conditions of human renal allograft function deterioration, 33 renal transplant biopsies were performed 1-30 months after transplantations. Forty-one patients observed were on immunosuppressives: Pr, Aza, CsA following renal transplantation from a living-related donor parent. The patients were divided according to their histologic diagnosis into the following groups: 1, 15 pts in acute rejection attack (AR); 2, 10 pts with cyclosporine nephrotoxicity (CsN); 3, 10 pts with chronic vascular rejecting kidney (ChR). A conventional histologic investigation and immunohistochemical analyses of CD3 and CD25 molecules were performed in groups 1-3. Spontaneous blastogenesis (SB) of peripheral lymphocytes was simultaneously determined and compared with the controls (C)-30 healthy people, and with patients with stable renal allograft function (S)-8 pts. The highest IL-2R expression on diffuse or focal dense MNC infiltrates in interstitium was observed during AR, without IL-2R+ MNC in glomeruli. Low to moderate focal interstitial infiltrates in damaged areas of renal parenchyma due to CsN, were IL-2R negative. In ChR, moderate IL-2R expression was observed on interstitial spare mild or focal dense MNC infiltrates with IL-2R expression present on glomeruli infiltrating MNC. Significant increases of SB values were recorded during the first week after transplantation and AR in comparison to C. The highest SB values were in ChR group. Values of SB in CsN and S were on the C and before transplantation levels.(ABSTRACT TRUNCATED AT 250 WORDS)

Biopsy↗
PubMed1987

Kidney pathology in endemic nephropathy.

Histopathologic analysis was made of kidneys from seven cases of typical endemic nephropathy and five cases of probable endemic nephropathy or upper urinary tract carcinoma from Yugoslavia. Glomeruli usually had an increased mesangial matrix and abnormal lobulation, sometimes with vacuolar degeneration. Particularly the straight ascending, descending and Henle loop tubules showed increased epithelial degeneration and regeneration. A network of interstitial scars and a subcapsular stratum of notable cortical degeneration contributed to severe renal atrophy. Unusual intrarenal vein thickening with or without obliteration was notable. Reactive glomerular, Bowman's epithelial and convoluted tubular alterations were ascribed to a filtered toxic material with carcinogenic properties which was considered eventually responsible for renal atrophy, fibrosis and some attributes of an autoimmune reaction.

Adult↗