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M Singer

Publications and source records attributed to M Singer.

At least 307 records · Page 17Linked to original sources
PubMed1975

Distribution of ultrastructural tracers in crustacean axons.

Ruthenium red and horseradish peroxidase were used to compare the uptake of exogenous molecules into crayfish motor axons and their sheaths in severed and intact peripheral nerves. Both tracers penetrated the axonal sheath and were subsequently seen lining small vesicles and tubules in the axoplasm. Tracer appeared to enter the axon via pinocytotic vesicles. There were no perceptible quantitative or qualitative differences in ruthenium red uptake between intact and severed axons. However, counts of tracer-filled vesicles in axons exposed to peroxidase showed that at least three times as much tracer penetrated the severed as opposed to the intact axons.

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PubMed1975

The interaction of histones with simian virus 40 supercoiled circular deoxyribonucleic acid in vitro.

The interaction of supercoiled, circular SV40 DNA with calf thymus histone fractions has been studied. Five- to ten-fold less f1 histone is required to complex a given amount of DNA compared to the other histones. When the supercoiled DNA is converted to either the relaxed circular form, or full length linear molecules, or gragmented linear or denatured stands, the efficiency of complex formation with f1 histone markedly decreases. We conclude that f1 histone has a special ability to interact with supercoiled DNA. This conclusion is supported by the fact that supercoiled circular Col E1 DNA interacts with f1 as efficiently as does SV40 DNA.

Animals↗
PubMed1975

The ultrastructure of Wallerian degeneration in the severed optic nerve of the newt (Triturus viridescens).

Wallerian degeneration in the severed newt's (Triturus viridescens) optic nerve is complete between the 10-14th post operative day (p.o.d.). Consequently, the newt optic nerve displays one of the most rapid degenerative responses yet reported for the central nervous system of vertebrates. In most cases it also exhibits the speed of degenerative phenomenon in the vertebrate peripheral nervous system. The degeneration of unmyelinated axons is most rapid and is completed by 2-3 p.o.d., compared to myelinated axons, most of which degenerate between 2-10 p.o.d. Myelin ring formation (vesicular transformation) is the principal form of lamellar breakdown and occurs in a highly organized manner which can be clearly staged. The glial cell response to Wallerian degneration is two-fold: cytoplasmic hypertrophy and myelin-lytic. Glial hypertrophy subsides by the 10-14 p.o.d. with the ingrowth of numerous regenerating nerve fibers. The myelin-lytic response accounts for most of the myelin destruction. Leukocyte-like and microglia-like cells also participate in myelin breakdown but to a lesser degree.

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