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Biomedical subjects

M Singh

Publications and source records attributed to M Singh.

At least 1,009 records · Page 56Linked to original sources

The effects of three training intensities on VO2 max and VE/VO2 ratio.

This study examined the effects of three training intensities on the maximum oxygen uptake (VO2 max) and ventilatory equivalents for oxygen (VE/VO2 ratio) and carbon dioxide (VE/VCO2 ratio) at submaximal and maximal workloads. Forty males, classified as high or low fit according to their relative VO2 max, were assigned to one of four subgroups: a control group that did not train; a continuous training group that trained at an oxygen uptake that was 10% above that at which the VE/VO2 ratio reached a minimum; another continuous training group that trained at an oxygen uptake that was mid-way between that at which the VE/VO2 ratio reached its minimum and maximum; and an interval training group which trained at 100% VO2 max. The work:rest intervals were one minute each. Initial fitness level and total training power output for the eight week program was equalized in each group in the two fitness categories. The results indicated that both fitness categories responded similarly to the three training intensities. The training groups showed significant but equivalent increases in the VO2 max, but no significant changes in the max VE/VO2 or max VE/VCO2 ratios. The submaximal VE/VO2 and VE/VCO2 ratios were significantly lowered, primarily as a result of a significantly lowered, primarily as a result of a significant decrease in the ventilation volume. It was concluded that continuous and interval training programs wee equally effective in improving VO2 max and submaximal ventilatory efficiency, regardless of initial fitness level, provided the total amount of work completed was equalized. Such training programs, however, were unable to modify the maximal ventilatory efficiency in either fitness category.

Carbon Dioxide↗

Gallium-68 lipophilic complexes for labeling platelets.

Generator produced 68Ga-labeled platelets could be useful for positron emission tomographic (PET) studies of thrombosis or atherosclerosis. To label platelets with 68Ga, we have studied the effects of trace metals in elutions of 68Ga from 68Ge. Studies were conducted on the formation of lipophilic 68Ga complexes 8-hydroxyquinoline, tropolone, and mercaptopyridine-N-oxide (MPO). Parameters such as pH, buffers, concentration of ligand, and stability with time were investigated. High performance liquid chromatography and instant thin layer chromatography were used to quantitate formation of the 68Ga complex. Platelets from human, dog, and rabbit plasma were incubated with the 68Ga complexes and the percent labeling determined. Accumulation of platelets in the catheter scraped aorta of the rabbit was determined by PET imaging, tissue counting, and autoradiography. Gallium-68 MPO gave 40-60% labeling of rabbit platelets with higher accumulation in the scraped aorta compared to the normal.

Animals↗

Romberg's syndrome: a case report.

A first case of Romberg's syndrome from Libya is reported. Romberg's syndrome is a rare disease causing progressive facial hemiatrophy. It was first described by Parry in 1825 and then by Romberg in 1846. Since then there have been sporadic case reports and reviews in the literature [1-5]. For the first time, we report a case from Libya.

Child↗

Metabolic and cinematographic analysis of walking and running in men and women.

The purposes of this study were to compare the total metabolic costs and gait patterns of walking and running at self-selected, comfortable speeds in males and females. Total oxygen consumption was used to determine the metabolic cost, and cinematographic analysis was used to study the gait patterns of walking and running a distance of 1 km in 12 male and 12 female subjects. No significant sex difference was observed for the speed, vertical lift per stride, and total vertical lift per km of distance walked. Females ran at a significantly slower speed than males (P less than .01), but no significant sex difference was observed for the vertical lift per stride or total vertical lift per km of distance run. In both sexes, the gross and net energy costs of running were significantly greater (P less than .001) than those of walking when values were expressed as kcal . kg-1 . km-1 or cal . kg-1 . stride-1. No significant sex difference was observed in the gross or net metabolic cost of walking, whereas during running, the gross and net metabolic costs in kcal . kg-1 . km-1 were significantly higher (P less than .05) in females than in males. It was hypothesized that this sex difference was due to the cumulative effect of several factors which were biomechanical and metabolic in nature.

Adult↗

Functionalized polymeric liposomes. Efficient immobilization of alpha chymotrypsin.

A polymerizable phospholipid has been synthesized having a latent aldehyde moiety in the head group (1). Photopolymerized vesicles which have been prepared from this phosphatidylcholine have been successfully conjugated to alpha chymotrypsin. A high degree of loading was achieved with significant retention of enzymatic function.

Chymotrypsin↗

Clinical validation of enzymeimmunoassay of human luteinizing hormone (hLH) in the detection of the preovulatory luteinizing hormone (LH) surge in urine.

The preovulatory luteinizing hormone (LH) surge (mean, 60.7 standard error +/- 4.7 mIU/ml) as determined by a solid-phase enzymeimmunoassay in urine has been correlated with clinical parameters in 24 women. In group A, of seven women, the preovulatory LH surge correlated with basal body temperature and cervical mucus. In one of the women in group A, serum levels of pituitary and gonadal hormones confirmed ovulation. In group B, of 17 women, the urinary estrone-3-glucuronide (E1-3-G) peak was either coincident with or preceded the LH surge. The LH surge in all cases occurred 12 to 24 hours prior to follicular rupture, as visualized by real-time sonography. The enzymeimmunoassay for the detection of the preovulatory LH surge is useful in patients for artificial insemination and for aspiration of mature oocytes for in vitro fertilization.

Adult↗