Biomedical subjects

M Sisto

Publications and source records attributed to M Sisto.

21 records · Page 2Linked to original sources

PubMed1993

Varicella-zoster virus DNA in granulomatous skin lesions following herpes zoster. A study by the polymerase chain reaction.

Granulomatous reactions at sites of previous cutaneous herpes zoster lesions occur, but their etiology is not known. Three tissue specimens from 5 cases identified clinically and histologically as post-zosteric granulomatous reactions were studied for the presence of varicella-zoster virus (VZV) deoxyribonucleic acid (DNA) by the polymerase chain reaction using specific primers for VZV. VZV DNA was detected in 1 of 3 cases where the granulomatous reaction occurred immediately in the wake of resolving vesicular herpes zoster lesions. Finding viral DNA in earlier reactions probably represents residue from the active herpetic process. VZV DNA was not identified in granulomatous reactions arising between 1 month and up to 4 years after resolved herpes zoster. The negative result in these cases supports the hypothesis that there is no association between persistence of VZV DNA and granuloma formation. How long VZV DNA is detectable at sites of resolved herpes zoster lesions could be the subject of further studies.

Aged↗

PubMed1993

Onychomycosis: successful treatment with once-weekly fluconazole.

We report a case of fingernail onychomycosis in an immunosuppressed renal transplant patient who was successfully treated with once-weekly fluconazole therapy. Relapse did not develop over the 2-month follow-up period. There were no adverse effects from therapy and the cyclosporin dose did not require adjustment. This case suggests that fluconazole may play a role in the treatment of onychomycosis with once-weekly dosing schedules. Future large-scale clinical trials are indicated to fully evaluate its safety and efficacy in onychomycosis.

Drug Administration Schedule↗

PubMed1992

PCNA expression in cutaneous keratinous neoplasms and verruca vulgaris.

Using an antibody to PCNA and a standard immunohistochemical system, the authors examined normal epidermis and cutaneous neoplasias for expression of PCNA, a protein associated with DNA polymerase delta and DNA replication. In squamous cell carcinoma in situ (SCCI), a unique expression of PCNA, which frequently involved the nuclei of all keratinocytes within the lesion, was found. Heaviest staining was in the uppermost layers of the epidermis. PCNA expression ended abruptly at the histologic margin of the lesion. Because SCCI can be associated with the presence of human papillomavirus (HPV) DNA, the authors evaluated PCNA expression in verruca vulgaris and found a pattern similar to that in SCCI. Assuming that PCNA expression in these two lesions is related to cell division, the authors hypothesize that the mechanisms that control proliferation in SCCI may be similar to those operative in verruca vulgaris.

Carcinoma, Squamous Cell↗