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Biomedical subjects

M Sleigh

Publications and source records attributed to M Sleigh.

5 recordsLinked to original sources
PubMed1994

The role of PDGF-BB on the development of the collateral circulation after acute arterial occlusion.

The survival of tissues in the presence of arterial occlusion is critically dependent on the development of collateral blood vessels. Identification of the biochemical mediators and their mechanism of action is fundamental to an understanding of the evolution of the collateral circulation. The ability of PDGF-BB to promote this was evaluated in an animal model of hind limb ischaemia. In order to obtain significant quantities of this mitogen for use in our animal model, human recombinant PDGF-BB was expressed in a Chinese Hamster Ovary (CHO) cell line. The transfected CHO cells produced 544 micrograms/l of PDGF-BB in serum free medium (SFM). The 30 kDa form of PDGF-BB was purified to homogeneity as judged by silver staining and amino-acid sequencing. Purified PDGF-BB was shown to be bioactive by a cell proliferation assay. The exogenous administration of PDGF-BB enhanced the recovery of blood flow after acute arterial occlusion. The results suggest that PDGF-BB may have therapeutic value in promoting collateral development following arterial occlusion.

Acute Disease↗
PubMed1977

Ciliary function of the frog oro-pharyngeal epithelium.

The palate epithelium of the frog was examined by scanning electron microscopy, light microscopy and high speed cine micrography. The cilia remain stationary for much of time in the end-of-effective stroke position. Each beat cycle begins with a forwardly-directed recovery stroke lasting about 60 ms, followed by an effective stroke towards the oesophagus lasting about 12 ms. Activity can often be correlated with the presence of mucus, which is carried as strands on the tips of the ciliary effective strokes whilst the recovery strokes move beneath the mucus. Coordination of ciliary activity was very variable; local antiplectic metachrony of the recovery strokes could almost always be seen, and on very active epithelia effective strokes were associated with approximately diaplectic waves (either to left or right), but any particular pattern of coordinated activity was transient and quickly transformed to another pattern. Beating and coordination of these short cilia were compared with those of cilia propelling water.

Animals↗
PubMedDate not supplied

Antibody-based therapeutics--the third generation.

Peptide Technology Limited (Peptech) recently announced in Australia that its associate company, Cambridge Antibody Technology (CAT) had obtained rights to the commercial development of a new kind of engineered antibody molecule. Diabodies were pioneered by Dr Greg Winter of the Medical Research Council Laboratories in Cambridge. In this article, the origins and potential of diabodies are examined in the context of other recent developments in this very fast moving field.

Antibodies, Monoclonal↗