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M Soloviev

Publications and source records attributed to M Soloviev.

13 recordsLinked to original sources
PubMed1998

Characterization of human and rat brain myristoyl-CoA:protein N-myristoyltransferase: evidence for an alternative splice variant of the enzyme.

Using 5'-rapid amplification of cDNA ends, we have identified an extended 5'-end of mRNA coding for human myristoyl-CoA:protein N-myristoyltransferase (NMT). PCR using primers based on this new 5'-sequence and reverse primers within the currently accepted coding sequence of the enzyme resulted in the identification of a novel splice variant of NMT. In vitro translation of these cDNAs resulted in the production of proteins with apparent molecular masses of 63 kDa and 48 kDa. Immunoprecipitation of NMT from human cell lines and immunoblotting of a range of rat tissues has identified proteins with molecular masses corresponding to those derived from these cDNAs, and provided evidence that their relative abundance differs among tissues. Our results provide evidence that this enzyme exists in different forms resulting from alternative splicing of the mRNA.

Acyltransferases↗
PubMed1995

Lactate and pyruvate isotopic enrichments in plasma and tissues of postabsorptive and starved rats.

It has been proposed that plasma pyruvate isotopic enrichment (IE) during infusion of labeled lactate could be used to estimate the intracellular IE of lactate and pyruvate and thus to calculate their turnover rate. We determined the relations of plasma and tissue IE of lactate and pyruvate in anesthetized rats infused with [3-13C]lactate in an artery and sampled from a vein (A-V mode) or infused in a vein and sampled from an artery (V-A mode). In both groups of rats, the ratio of tissue to plasma lactate IE was < 1 with large differences between tissues: the highest ratio was observed in heart and the lowest in soleus. With the exception of liver, this ratio was higher in the A-V than in the V-A mode. Pyruvate IE was lower than lactate IE in tissues, with a few exceptions, and in plasma. This ratio of pyruvate to lactate IE was approximately 0.70 in plasma in A-V and V-A modes. Moreover pyruvate IE was also always higher in plasma than in tissues. This seemingly surprising result could be explained by the production of labeled pyruvate from labeled lactate inside the circulation by erythrocytes, because we observed a rapid isotopic equilibrium between lactate and pyruvate in blood "in vitro." Apparent lactate turnover was higher in the A-V than in the V-A mode when it was calculated using lactate as well as pyruvate IE. Therefore plasma pyruvate IE cannot be used in rats to estimate tissue IE and did not reconcile turnover rates measured using the A-V or V-A mode.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMedDate not supplied

[Staphylococcal infections in the Cluj-Napoca Clinic of Infectious Diseases during the years 1967-1972].

Over a six year period, in the Clinic of Communicable Diseases of Cluj Napoca, 2301 patients with staphylococcal infections were admitted to the Clinic, representing 8% of the total number of patients admitted, and 3513 staphylococcal strains were isolated. A number of 43 of the 2301 patients died (1.8%), but staphylococcal infection was actually the cause of death in only 35 cases (1.5%) (septicemia, staphylococcal meningitis and pulmonary infections). Eight of the patients died from the basic disease (hepatitis, tetanus, paratyphoid C fever etc.). A number of 2246 Staphylococcus hemolyticus aureus, 80 non-hemolytic Staphylococcus aureus and 162 Staphylococcus albus strains were isolated; most of the strains were resistant to antibiotics in different proportions.

Humans↗
PubMedDate not supplied

[Current aspects of infectious mononucleosis].

The authors discuss present aspects in infectious mononucleosis, with reference to the cases admitted to the Clinic of communicable Diseases of Cluj-Napoca, i.e. 115 cases during the 1954--1973 period (3 to 13 cases a year). In most cases the clinical symptomatology was mild and the laboratory tests typical not giving rise to any diagnostic difficulties. However, 28 cases (25%) presented jaundice, with positive hepatic tests, so that a differential diagnosis was established with viral hepatitis. In 9 cases nervous manifestations were reported: 4 encephalitis, 1 neuritis of the hypoglossal nerve, 2 facial paralysis, 2 benign serous meningitis cases, all of which fully recovered. The Paul Bunnel-Hăngănuţiu test was positive in titers of 1 : 40 -- 1 : 1280, in all the cases. Corticotherapy was applied with good results in 24 cases. All the cases ran a favourable course. On admission, 45% of the patients came with a diagnosis of infectious mononucleosis; the others were admitted with a diagnosis of : diphteric angina, viral hepatitis, influenza, German measles, mups, typhoid fever, prolonged febrile syndrome.

Adolescent↗