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Biomedical subjects

M Soutorová

Publications and source records attributed to M Soutorová.

At least 19 recordsLinked to original sources
PubMed1995

Correlations between parameters of body selenium status and peripheral thyroid parameters in the low selenium region.

The following were measured in 380 male and female inhabitants of Prague (n the age range 6-65 years):selenium in serum and urine, iodine in urine, thyroid-stimulating hormone, thyroid volume, thyroxine (T4), triiodothyronine (T3), ankle jerk time, pulse rate and body fat. Correlations were calculated to assess the influence of Se upon the thyroid hormone parameters and their peripheral effects in Se-deficient regions both by using the linear correlation analysis and by using the multiple linear correlation analysis and the analysis of variance. Many significant linear correlations were found for whole groups of examined persons, for the individual groups (boys, men, girls and women) as well as for subgroups, according to the age and sex. We conclude, from the analytical results of Se indices (serum, hair, urine) reported previously, and on the basis of the statistical demonstration of the influence of selenium upon thyroid hormone levels and the peripheral effects in the group of Prague inhabitants in connection with the previously documented role of Se in pathways resulting in the synthesis of physiologically active thyroid hormone, that inhabitants of Prague are selenium deficient. Concurrent deficiency of selenium may modify and even worsen iodine deficiency disorders of various stages. Multiple linear correlation analysis followed by analysis of variance of subgroups indicated a coincidence of the effects of some measured parameters upon the peripheral manifestation of thyroid hormone state as well as highlighting the effect of other independent variables of thyroid hormone metabolism than those measured in the study.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1995

Osteocalcin in congenital adrenal hyperplasia.

Osteocalcin in the serum reflects bone turnover. It is known that prolonged therapy with glucocorticoids inhibits bone turnover. The aim of this study was to evaluate the osteocalcin level in children with congenital adrenal hyperplasia treated by glucocorticoids and mineralocorticoids and to assess the influence of 1,25(OH)2D3. The subjects were 75 children with congenital adrenal hyperplasia, aged 1-18 years, treated with glucocorticoids and mineralocorticoids in substitution doses from birth. These children demonstrated low levels of osteocalcin and alkaline phosphatase, whereas calcium and phosphate were in the normal ranges. Despite these abnormalities, no osteoporosis was detectable and a normal growth rate was confirmed, most probably because of higher levels of androgens; 17-OH progesterone averaged 11.8 nmol/l. After treatment with 1,25(OH)2D3, the osteocalcin levels increased, followed later by increases of alkaline phosphatase and bone isoenzyme.

Adrenal Hyperplasia, Congenital↗
PubMed1992

[Diagnostic significance of osteocalcin levels in the classification of senile osteoporosis].

The authors investigated the osteocalcin (BGP) serum level in 55 women with the senile type of osteoporosis. According to BGP values they divided the group into three sub-groups. Lower values were recorded in 27 subjects (50%), normal values comparable with a control group of 13 healthy women of equal age were recorded in 21 subjects (38%) and in a small sub-group of 7 women (12%) the BGP level was raised or high. Senile osteoporosis can be also heterogeneous, characterized by a reduced, normal but in some also an elevated metabolic turnover of bone. It is, however, possible that this is a question of different stages and at the time of examination the mentioned status was recorded. An opportunity to examine BGP levels would be a great asset for osteological departments, among others also for the classification of osteoporosis with regard to the rate of the metabolic turnover or remodelling activity and would provide doctors with valuable information for decision taking on rational therapy.

Aged↗
PubMed1992

[Multiple endocrine neoplasia type 2: familial variant of medullary carcinoma of the thyroid gland].

In addition to a brief characteristic of the syndrome of multiple endocrine neoplasia type 2 and medullary thyroid carcinoma with a familial incidence which is a prerequisite of the syndrome, the authors submit an account on a group of 53 patients who were on the authors' records during the past 12 years. During this period the disease is systematically searched for in the families of newly diagnosed patients by examining the immunoreactive calcitonin level of relatives. Familial variants account for 28% of all medullary thyroid carcinomas. Patients who are on the records so far belong to 24 families. Approximately twice as often an isolated variant of the familial type of medullary carcinoma is involved, as compared with association with another endocrine affection, in particular pheochromocytoma (Sipple's syndrome), but associated forms will increase in number perspectively (multiple endocrine neoplasia 2A). The syndrome of multiple endocrine neoplasia 2B is very malignant but in view of the typical phenotype the disease should be diagnosed already before the change to malignancy--once the disease develops into the clinical stage the course is very adverse. From the original number of all familial tumours 38 subjects survive (72%), incl 22 who were subjected to bilateral total thyroidectomy based on screening in the preclinical stage. The prognosis of these individuals is very favourable, the calcitonin levels are throughout the follow-up period (2-10 years) repeatedly negative. With regard to the possible association with another endocrinopathy (pheochromocytoma or hyperparathyroidism) all must be followed up systematically (screening) with regard to the manifestation of an associated endocrinopathy frequently only after a longer time interval.

Adult↗
PubMed1992

[Medullary carcinoma of the thyroid gland. Personal experience].

The authors present their experience with the diagnosis and treatment of medullary thyroid carcinoma (MTC) in a group of 175 patients in the records of the RI in Motol. From the above number 106 are alive, 69 of the patients who died were diagnosed at the time of the clinical manifestation of the disease, frequently an advanced stage of the disease. Of the living patients one third was treated by total thyroidectomy after active screening. MTC accounts under our conditions in recent years for 8.6% of all thyroid malignancies. The familial variant with autosomal dominant heredity accounts for 26.5% of the above number. Within the framework of the familial variant the most frequent finding is non-MEN MTC, only 30% MTC are part of the MEN 2A or MEN 2B syndromes. MTC in childhood belongs practically always to the familial variant. At present the authors' group comprises 13 children and adolescents under 18 years, incl. one boy with the MEN 2B syndrome who succumbed to the disease. In 8 children based on active screening total thyroidectomy was indicated, incl. 5 patients were by histological examination only hyperplasia of the C-cells, i.e. a precancerous condition, was detected. After operation repeatedly in all these patients the level of immunoreactive calcitonin is low which indicates an excellent prognosis. The authors draw attention to the importance of active screening in families of all direct relations of the patient where MTC was diagnosed. Complete removal of thyroid tissue at the time of hyperplasia of C-cells or the presence of tumourous microfoci has a marked positive effect on the subsequent fate of the patients.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1992

[Determination of serum calcitonin using a non-commercial RIA method].

A radioimmunoanalytical determination of the immunoreactive calcitonine in the humen serum was worked out using the authors' own specific antiserum, the preparation and properties of which are reported in the present paper. The precision and reliability of the analytical procedure is within the usual limits (the intraserial variation coefficient was 8.6%, the calculated sensitivity was 6 pg/ml). The reported procedure was employed to determine calcitonine in a larger number of patients who were examined because of suspected medullar carcinoma of the thyroid gland and in patients suffering from other thyropathies. The results are discussed from the viewpoint of heterogeneity of calcitonine in the circulation.

Calcitonin↗
PubMed1991

Application of non-commercial methods for estimation of immunoreactive serum calcitonin in clinical practice (comparison of two methods).

The authors compared the results of two radioimmunological methods for the estimation of immunoreactive calcitonin in human serum. Parallel with the commercial RIA kit the estimation was made by the authors' own modification of this process with their own specific antiserum and radioligand prepared in the laboratory. The results in a large group of patients with medullary carcinoma of the thyroid gland (MCT) in different stages of the disease revealed that although the values obtained by the two methods differ, there is a statistically significant correlation between the values and the clinical evaluation is also comparable.

Calcitonin↗
PubMed1990

[Comparison of 2 methods for determination of antibodies to thyroglobulin in clinical practice].

The authors compared the results of two methods for the estimation of antibodies against thyroglobulin: the principle of the Thymune-T test (Wellcome) is a haemagglutination method; parallel examinations were made by the author's modification of the radiochemical coprecipitation test (RCT). The laboratory results were evaluated in a large clinical group of patients with different types of thyroid disease. It was confirmed that the haemagglutination method gives more frequently positive results than the radiochemical test, in particular in the range of low titres. The chi square test provided, however, evidence that between methods a significant correlation exists. Although the results of the two tests are not necessarily identical in individual patients, it may be stated that RCT is an adequate method of choice, as it is simpler, more rapid and cheaper, and that it can be done in large series with objective automated evaluation of data.

Adult↗
PubMed1989

Plasma somatostatin activity in medullary cancer of the thyroid.

In a group consisting of 50 adults and 5 children with diagnosed or suspected medullary cancer of the thyroid, plasma somatostatin levels were measured in 77 samples with a sensitive radioimmunoassay. Only 2 patients had clearly enhanced plasma somatostatin immunoreactivity, both with highly active aggressive tumors. Other patients had plasma somatostatin levels of under 20 ng/l or undetectable ones. No evidence of response of somatostatin levels to pentagastrin stimulation was found.

Adolescent↗
PubMed1981

Iodine metabolism in mothers of children with cleft lip and/or palate anomaly and in persons with endemic goitre.

The authors examined 106 subjects with simple goitre and 18 hyperthyroid subjects who themselves or their ancestors came from endemic areas, furthermore 31 mothers of children with cleft lip and/or palate and 50 subjects acting as controls. The biochemical indicators in endemic goitre were at the borderline of hypothyroidism, in mothers of children with clefts on the borderline of hyperthyroidism. The authors conclude that genetically conditioned endemic mild thyroid hypofunction still persists in our population nad that it must be taken into consideration during the examination and treatment of thyropathies. The thyroid metabolism of mothers of children with cleft lip and/or palate differs significantly in many indicators from the endemic group and preconceptional and early prenatal prevention should be focused on elimination of possible marginal hyperthyroidism. The necessity to examine the thyroid metabolism of the mother as part of preconceptional genetic care was confirmed by these results.

Adult↗
PubMed1979

Labelled amino acids in plasma of patients with thyrotoxicosis and thyroid cancer after radioiodine treatment.

The levels of total organically bound 131I, per cent of labelled iodoproteins, total labelled iodinated amino acids and a percentage of individual iodinated amino acids (thin-layer chromatography) were measured in serum of 84 patients with thyroid cancer and of 16 patients with thyrotoxicosis at 48 h after the administration of therapeutic dose of 131I. In thyrotoxic patients treated with therapeutic doses of 131I (2 to 39.6mCi; 74 to 1500 MBq) the findings were similar to normal subjects. In patients with thyroid cancer a significant increase of iodotyrosines was found after thyroid radioiodide ablation (100 to 200 mCi; 3.7 to 7.4 GBq). No remarkable differences were found between two groups of patients with thyroid cancer, the first one being treated with thyroid eliminating dose in attempt to activate the metastases of functionally differentiated tumour, while the second one was treated with similar doses (i. e. 100 to 200 mCi) to suppressor destroy a functionally active tumour or its metastases. Even though the hormonogenesis in tumours was hardly distinguishable from the products of its radiation damage, it was suggested that the hormonogenesis in neoplastic tissue differs from that in normal thyroid only quantitatively, being less in patients with thyroid cancer than in these with thyrotoxicosis.

Amino Acids↗
PubMed1975

Serum thyroxine estimation: clinical evaluation of a modified semiautomated method and its ability for differential diagnosis of thyroid diseases.

A modified semiautomated microchemical method for determining serum thyroxine has been described. It consists of the isolation of T4 on a column of cation exchange resin Dowex 50 W X 2 and of a fully automated determination of iodine in the ammoniacal eluate from the column after an acidic mineralization. The diagnostic value of the method has been evaluated on a small group of patients examined for suspected thyroid gland dysfunction, in comparison with a parallel determination of T4 by a CPBA method (Tetrasorb test kit). From the results it follows that the microchemical method gives the results something inferior in comparison with the CPBA technique. The differences of means between the basic diagnostic groups of patients (euthyroids, hypothyroids, and hyperthyroids) in both methods were statistically significant. The extent to which these tests can discriminate was investigated at a 5% discrimination threshold. The discriminatory ability of both methods between hyperthyroidism and euthyroidism is nearly the same. The CPBA method discriminates euthyroids from the hypothyroids better, the results of both tests, however, do not differ too much. It seems to be confirmed that irrespective of the method used, the determination of serum T4 itself can detect neither hyperthyroidism nor hypothyroidism reliably. The possible causes are discussed in this paper.

Autoanalysis↗