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Biomedical subjects

M Spacek

Publications and source records attributed to M Spacek.

15 recordsLinked to original sources
PubMed2006

Nuclear myosin is ubiquitously expressed and evolutionary conserved in vertebrates.

Nuclear myosin I (NMI) is a single-headed member of myosin superfamily localized in the cell nucleus which participates along with nuclear actin in transcription and chromatin remodeling. We demonstrate that NMI is present in cell nuclei of all mouse tissues examined except for cells in terminal stages of spermiogenesis. Quantitative PCR and western blots demonstrate that the expression of NMI in tissues varies with the highest levels in the lungs. The expression of NMI is lower in serum-starved cells and it increases after serum stimulation. The lifespan of NMI is longer than 16 h as determined by cycloheximide translation block. A homologous protein is expressed in human, chicken, Xenopus, and zebrafish as shown by RACE analysis. The analysis of genomic sequences indicates that almost identical homologous NMI genes are expressed in mammals, and similar NMI genes in vertebrates.

Amino Acid Sequence↗
PubMed2005

[Laparoscopic resection of the abdominal aortic aneurysm].

Some 500 aortoiliac aneurysms are mostly electively operated in the Czech Republic each year. Miniinvasive approaches already widely accepted in general surgery are gradually becoming more routine in vascular surgery as well. Better patient comfort hand in hand with reduced hospital stay represent the major advantages of these procedures. The authors present their initial experience with laparoscopic AAA resection with the use of their own modification of the transperitoneal-retroperitoneal approach. Ischemic preconditionning's techniques with regard to the expected prolonged aortic clamping time were used during surgery.

Aorta, Abdominal↗
PubMed2004

[Laparoscopic ilio-femoral by-pass. A case-review].

The authors introduce a laparoscopically conducted ilio-femoral by-pass operation for the external iliac artery occlusion. The common pelvic artery preparation, the clips application, the arteriotomy and the central anastomosis, all were conducted laparoscopically, the peripheral anastomosis was then carried out through a standard inguinal incision. The laparoscopic approach represents, together with angioplasties and stentgraft implantations, yet another technical variation in vascular surgery.

Femoral Artery↗
PubMed2004

[Laparoscopic vascular reconstructions--initial experience].

Laparoscopic techniques, which have been found useful in the general surgical procedures, have influenced the vascular surgical procedures with a certain delay. The main reason for the delay were technical difficulties of the vascular anastomosis, which resulted in a reluctant acceptance of the laparoscopic procedures by vascular surgeons. However, with respect to clear benefits of the miniinvasive procedures, including reduction of the postoperative pain, faster recovery rates and reduction of the total treatment costs, the laparoscopic procedures are finally being applied in the vascular surgery as well. The authors introduce operative results and outcome of ten laparoscopic vascular procedures for aortoiliac occlusive disease, which were conducted from September 2003 to April 2004. In five cases, iliofemoral bypasses were conducted, fourtimes unilateral aortofemoral bypasses (AF) and once an aortobifemoral bypass (ABF). All the IF and AF reconstructions were conducted via laparoscopy and the ABF reconstruction was conducted as laparoscopy-assisted.

Aged↗
PubMed1989

[Transient ischemic attacks in the carotid drainage area].

The 63-membered total of patients is referred to with clinical signs of transient ischaemic attacks (TIAs) in carotid supplied area in account to the risky factors, results of selective auxiliary testing methods as well as the disease prognosis providing harmful occurrence of cerebral palsy completed.

Aged↗
PubMed1989

[Correlation of Doppler echocardiography and EEG findings in stenotic processes in the carotid arteries].

Results obtained from EEG examination and Doppler sonography in a group of 155 patients were studied and special attention was paid to ischemic cerebral lesions in stenotic processes in carotid arteries. Pair correlations of individual factors were statistically analyzed using a computer, i. e., results obtained from EEG, Doppler sonography, patients' age and final diagnosis. High statistical mutual dependence of both the methods was found which may be explained by the functional approach pursued in both the procedures. The contribution of both the methods to rapid and considerate diagnosis of vascular cerebral lesions is emphasized here.

Adolescent↗
PubMed1989

[Significance of EEG findings in administering anticonvulsant drugs to adults with compensated epilepsy which began in childhood].

Significance of EEG findings for prognosis of further development in paroxysm is evaluated in this work in adult epileptic patients suffering from seizures which appeared in their childhood. The assessment is based on EEG made during the active phase of the disease and in the time when fits were compensated. Seizureless condition after discontinued medication was observed only in a group of patients with secondary type of epilepsy. We suppose these patients should remain under control since two thirds of them showed diffuse nonspecific changes, which means 50% risk of recurrence of seizures after discontinuation of drug therapy. When medication continued, 55% of patients remained without any fits, of this number in 21.6% seizures recurred on the average within seven years after experimental discontinuation of drug therapy in spite of frequently normal EEG findings. The fact that the group of compensated patients without medication did not include those with primarily generalized seizures can be explained by their good conditions observed even in their childhood and for this reason most of them were not treated in the Anti-Paroxysmal Centre.

Adult↗
PubMed1989

[Lateralization of lesions in the carotid bed in patients with sudden cerebrovascular stroke].

Statistical significance of different factors in laterality of lesions in the carotid bed was studied on a group of 170 patients indicated to ultrasound examination. These were factors contained in the patients' history, i.e. ischemic heart disease, arterial hypertension, diabetes mellitus, age and sex of patients as well as results obtained from auxiliary examinations--Doppler sonography, KAG, EEG and CT of the brain. A marked statistical predominance of lesions in the left-side carotid bed was found, which was, however, true only for men, especially those after a completed stroke. The left-sided bed is, moreover, involved earlier, higher incidence of ischemic heart disease was also observed in left-sided lesions as well as more severe findings obtained from Doppler sonography, angiography, EEG and CT of the brain.

Carotid Arteries↗
PubMed1986

Method for characterization of selectivity in reversed-phase liquid chromatography. III. Retention behaviour in gradient-elution chromatography: application to the chromatography of pesticide compounds.

The method introduced recently for the characterization and prediction of absolute and relative retention in reversed-phase liquid chromatography under isocratic conditions has been applied to gradient-elution chromatography. The method is based on two indices (one lipophilic, nce, and the other polar, qi, to characterize the behaviour of each solute in methanol-water mobile phases of various compositions. The indices nce and qi of phenylurea and triazine herbicides were found to be composed of additive contributions of structural elements (functional groups) in the molecules of the solutes. Using the present approach, it is possible to predict retention volumes in gradient-elution chromatography with a precision comparable to those under isocratic conditions (i.e., less than 10% relative for most compounds tested). Simultaneous adjustment of the initial mobile phase composition and the slope of the linear gradient makes it possible to control to a certain extent the selectivity in gradient-elution chromatography using binary solvent gradients and offers an alternative approach to the use of multi-solvent gradients for selectivity control. This method may be especially useful in optimizing gradient-elution separations of pairs of compounds with positive differences in both indices nce and qi, delta nce greater than 0 and delta q greater than 0. Selection of the optimal gradient profile is illustrated on a practical example of gradient-elution chromatography of phenylurea herbicides.

Chromatography, Liquid↗