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Biomedical subjects

M Spitz

Publications and source records attributed to M Spitz.

44 records · Page 3Linked to original sources
PubMed1981

A hybridoma cell line secreting antibody to poliovirus type 3 D-antigen: detection in virus harvest of two D-antigen populations.

A mouse hybridoma cell line (Mo56) secreting IgG antibody to poliovirus type 3 D-antigen was obtained by fusion of a mouse myeloma cell line with spleen cells from mice immunized with Saukett virus. The monoclonal antibody was specific for Saukett virus strains in virus-neutralization and single-radial-diffusion tests. In immunoprecipitation tests the monoclonal antibody reacted with intact infectious virus particles (155S) and with a previously undescribed 70S poliovirus particle with D-antigenic reactivity.

Animals↗
PubMedDate not supplied

The human fetal auditory evoked potential.

The brain-stem auditory evoked potential (BAEP), a sensitive test of the functional status of the neonatal brain, has not been studied in utero since no practical technique for human fetal recording is available. We have developed a simple recording technique which allows continuous monitoring of the fetal AEP during labor. Waves I, III and V of the fetal brain-stem AEP have been consistently identified. Wave form morphology, interpeak latencies, and latency-intensity relations are similar to postnatal recordings. Middle latency potentials have also been recorded, with wave forms that correspond to the neonatal middle latency AEP.

Brain Stem↗
PubMedDate not supplied

Antigenic characterization of poliovirus type 3 using monoclonal antibodies.

Hybridoma cell lines secreting monoclonal antibodies to type 3 poliovirus were prepared, and their reactivity with infectious virus (D antigen), empty particles (C antigen), and isolated virion capsid proteins ( VPs ) were examined. Eight antibodies reacted with epitopes common to D and C antigens, and all of these possessed high titers of neutralizing activity. However, only 12 of 19 antibodies that reacted exclusively with D antigen neutralized virus infectivity, and some of these reacted only with strains of virus with T1-oligonucleotide maps identical or similar to that of Sabin vaccine polio virus. These antibodies will be of value in identifying strains of virus derived from Sabin vaccine. None of the 20 monoclonal antibodies that neutralized type 3 poliovirus strains reacted in immunoblot experiments with isolated virion capsid proteins. However, six of the 24 antibodies that reacted only with noninfectious C antigen bound to VP1 and VP3, and three of these antibodies also reacted with proteins of poliovirus types 1 or 2. The lack of reactivity of neutralizing monoclonal antibodies with isolated viral proteins suggests that the antigenic properties of proteins are determined by their arrangement in the virus and not simply by amino acid sequence.

Animals↗
PubMedDate not supplied

Treatment decisions in paediatric dialysis: children's versus parental rights.

Treatment decisions in Paediatric Dialysis are often fraught with ethical questions about how to serve the best interests of children on dialysis, especially when parents refuse recommended treatment and opt for non-optimal interventions. This paper reviews the issues of children's rights, as guaranteed in the United Nations Covenant on Children's Rights and the Israel's Patients' Rights Law, and presents several case vignettes where conflict between children's rights for optimal treatment and parental desires arose. The paper concludes with policy recommendations to assure that children's expressed desires and medical needs are met, including the establishment of multidisciplinary committees to address the needs of children on dialysis and their families.

Adult↗