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Biomedical subjects

M Spitznas

Publications and source records attributed to M Spitznas.

At least 127 records · Page 7Linked to original sources
PubMed1986

Histochemical demonstration of hyaluronic acid molecules by alcian blue.

Specimens of vitreous humour (monkey eye), Wharton jelly (human umbilical cord) and commercial hyaluronates were immersed in buffered fixative solutions containing either aldehydes and Alcian Blue, or aldehydes and Alcian Blue with MgCl2 as electrolyte. Two MgCl2 concentrations were used, 0.025 M and 0.3 M. Immersion in both solutions induced formation of precipitates which were postfixed in OsO4, dehydrated and embedded for thin section electron microscopy. The use of the same fixative solution produced morphologically comparable precipitates from all three materials. The precipitates, especially after fixation in the presence of electrolyte, were composed of linear, unbranched filaments, frequently aggregated into bundles. The filaments were considered to be molecules of hyaluronic acid.

Alcian Blue↗
PubMed1986

[Quantitative control of the function of the corneal endothelium by fluorophotometry in the anterior eye segment].

Whereas specular microscopy yields mostly qualitative information, fluorophotometry furnishes quantitative data of endothelial cell function. Determination of fluorescein permeability of the endothelial cell layer reflects the function of the endothelial barrier. Following topical application of fluorescein, the time-dependent change in the fluorescein concentration in corneal stroma and aqueous yields the transfer coefficient (Kc) of the corneal endothelium. With the Fluorotron Master, Kc was 3.77 +/- 0.57 X 10(-3)/min. for normal eyes; patients with cornea guttata or Fuchs's dystrophy had a significantly higher transfer coefficient (Kc = 7.9 +/- 2.88 X 10(-3)/min.). Normal Kc values were found 6-63 months (average 33.5 months) after phacoemulsification with implantation of a posterior chamber lens.

Adult↗
PubMed1985

Lipofuscin accumulation in extraocular muscle of rats deficient in vitamins E and A.

The influences of vitamin E and A deficiencies on the formation of lipofuscin in two different muscle fiber types of the extraocular muscle were tested. Weanling female albino rats (Sprague-Dawley) were divided into three groups and fed purified diets that were adequate or deficient in vitamin E and A: +E, +A; -E, +A; and -E, -A. After 35 weeks on this diet, the animals were killed for analysis of extraocular muscle. When examined by fluorescence microscopy, the extraocular muscle of the (-E, +A) rats showed more lipofuscin-specific fluorescence than the (+E, +A) and (-E, -A) rats. Lipofuscin was then further analyzed by electron microscopy (EM), using morphometric analysis. By this high-resolution technique, the increased lipofuscin of the (-E, +A) extraocular muscle was seen to be confined mostly to the type I fibers. The type II fibers were quite insensitive to vitamin E deficiency: in type II fibers, the (-E, +A) and (-E, -A) muscle showed very small amounts of lipofuscin, and the (+E, +A) showed none at all. Vitamin A has an influence on vitamin E-deficiency and appears to be involved in the formation of lipofuscin in type I muscle fibers of the extraocular muscle.

Animals↗
PubMed1983

Hereditary posterior microphthalmos with papillomacular fold and high hyperopia.

Five patients had a bilateral hereditary ocular syndrome composed of posterior microphthalmos with a papillomacular fold and high hyperopia. Anterior segment dimensions were near normal; the vitreous compartment was markedly fore-shortened. A papillomacular retinal fold extending from the center of the fovea toward the optic nerve head was present. Visual acuity ranged from 0.05 (20/400) to 0.6 (20/33); refractive errors ranged from + 11.25 to + 17.50 diopters. An autosomal recessive pattern of inheritance is postulated.

Adult↗
PubMed1983

Motorized teleguided stereotactic micromanipulator for vitreous microsurgery.

A recently developed motorized stereotactic micromanipulator enhances the precision of the procedure, the comfort of the surgeon, and the safety of the patient during vitreous surgery. It can accommodate almost any type of vitreous surgical instrument by means of special adapters. The system is moved by the action of electrical motors remotely controlled by the surgeon operating a joystick on a separate control unit. Electrical reversal of motion, obtained by flipping a switch, allows observation through an altered version of the Panfundoscope that provides a 160 degrees inverted fundus view during all phases of the operation. In addition to improving many aspects of present vitreous surgical procedures, this stereotactic system may initiate the development of still finer surgical methods that cannot be performed with the free hand.

Fluorescein Angiography↗
PubMed1983

Nine cases of cavernous hemangioma of the retina.

The findings in nine patients with cavernous hemangioma of the retina confirmed the accepted characteristics of this malformation. Clusters of dark-red saccular aneurysms within the inner retinal layers were partly covered by a white epiretinal membrane. There was no clinical or angiographic evidence of exudation from the angioma and there was no growth during a median follow-up period of 6.3 years. The size of the aneurysms, the extent of the lesion, and the associated venous malformations varied greatly from case to case. Fluorescein angiographic findings during follow-up periods of as much as 16 years suggested progressive thrombosis and organization of the angioma.

Adolescent↗
PubMed1983

[Combination of local anesthesia and intubation anesthesia in ophthalmic surgery - a gentle anesthetic technic for high risk patients].

Local analgesia has its definite place in ophthalmic surgery, but is restricted to short-term procedures. Major surgery involving longer periods of manipulation, especially of the retina and vitreous body, usually require general anaesthesia. These patients, predominantly elderly and of reduced general health, are at risk especially during the postoperative period. Regaining consciousness and protective reflexes quickly is essential in this group. To reduce the dosage of potent general anaesthetics in a pilot study a special risk group received a combination of retrobulbar local analgesia and light general anaesthesia. It was found that with optimal local blockade of pain conduction, general anaesthetics could be reduced as compared to a control group receiving general anaesthesia alone. The special advantage of this combined anaesthetic technique became evident during the postoperative period, since foreshortened recovery of consciousness, complete orientation as to time and space allowed referral to the ward at an early date. Particularly advantageous in these patients at risk was the prolongation of analgesia in the operated eye well into the postoperative period.

Adult↗
PubMed1982

Dominant retinitis pigmentosa. A clinicopathologic correlation.

Autopsy and clinical studies were performed in a family with dominantly inherited retinitis pigmentosa. The eyes of two senior members of the family were obtained for histopathologic study, while three other family members were studied clinically. All family members studied had visual field loss, nyctalopia, and a spectrum of pigmentary disruption. There was variable expressivity of pigmentary migration, foveal atrophic changes, surface wrinkling retinopathy, choriocapillaris atrophy, drusen, vitreous synchysis, and optic pallor. Three specific zones of retinal and retinal pigment epithelial changes were identified histopathologically. A clinicopathologic correlation is presented.

Adolescent↗