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M Stalder

Publications and source records attributed to M Stalder.

29 records · Page 2Linked to original sources
PubMed1990

[Prekallikrein deficiency: apropos of 2 cases].

An isolated, considerably prolonged aPTT (117 and 112 sec respectively; normal range 26-36 sec) was discovered during the preoperative workup in 2 patients aged 48 and 66 years. Both had a negative personal and family history for bleeding. Levels of the intrinsic coagulation factors which potentially cause a bleeding risk (VIII, IX, XI) were normal, and an inhibitor of the aPTT could not be detected. Investigation of the contact phase revealed a severe functional deficiency (less than 1%) of prekallikrein (PK) which was diagnostic of the homozygous state. Complete correction of the aPTT after prolonged activation of the contact phase and after addition of 0.2 volume of normal plasma to the sample is suggestive of this diagnosis, which needs to be confirmed by determination of the functional PK. Though deficiency of PK does not carry a bleeding risk, it is important to identify such cases in order to avoid time-consuming investigations in a surgical emergency situation.

Adult↗
PubMed1987

[Lupus erythematosus, circulating anticoagulant and obstetric complications. Apropos of 2 cases].

Systemic lupus erythematosus (SLE) is a disease occurring mostly in women in the childbearing age. We present two cases of pregnancy complicated one by SLE, the other by a "lupus-like" syndrome. In the first case, the fetus suffered from a complete heart block; in the second one, a lupus anticoagulant was discovered in the mother. These two cases are discussed by the light of the literature.

Adult↗
PubMed1986

Peripheral neuropathy associated with high-dose Ara-C therapy.

Central nervous system toxicity associated with high-dose cytosine arabinoside (Ara-C) therapy (HD Ara-C) is well known. The authors report the case of a severe isolated peripheral polyneuropathy due to HD Ara-C. Electrophysiologic changes and histologic observations were consistent with axonal degeneration and scattered destruction of myelin sheaths. This observation emphasizes the need for careful complete neurologic evaluation for patients receiving HD Ara-C treatment.

Adult↗
PubMed1985

Release of vascular plasminogen activator (v-PA) after venous stasis: electrophoretic-zymographic analysis of free and complexed v-PA.

To better characterize the plasminogen activators present in the euglobulin fraction (EF) of plasma before and after venous occlusion, euglobulins of 30 healthy volunteers and of 32 patients with idiopathic thromboembolic disease were submitted to SDS-PAGE and zymographic detection. The patterns thus obtained were compared to the fibrinolytic activity (FA) of the EFs measured on fibrin plates. There were significantly more patients with a low fibrinolytic response (FR) to stasis (difference between FA after and before stasis less than or equal to 0.3 TAU/ml) than controls (P less than 0.01). The electrophoretic-zymographic analysis revealed that: (i) in the resting state vascular plasminogen activator (v-PA) is always present in a form exhibiting a mol wt of 110 000 (complex of v-PA with its fast-acting antiactivator); (ii) a high FR to stasis (greater than 1.3 TAU/ml) is always associated with the presence of free v-PA (68 kdalton band); (iii) free v-PA is never detected when the FR is low; and (iv) a poor FR is generally (15 of 17 patients with low FR) associated with apparently complete inhibition of the released v-PA by the fast-acting antiactivator; in the two remaining patients in whom no broadening of the 110 kdalton band is observed, the release mechanism for v-PA is probably impaired.

Adolescent↗
PubMed1984

[Mycosis fungoides and monoclonal gammopathy. Fortuitous or causal association?].

We report the case of a patient suffering from mycosis fungoides and plasma cell myeloma. It is postulated that this is not a fortuitous association, but that the B cell neoplasm evolved under the sustained inducer stimulus of the malignant T lymphocytes. This hypothesis is suggested by the demonstration of the helper phenotype of the cutaneous lymphocytes associated with a predominance of helper lymphocytes in blood.

Antibodies, Monoclonal↗
PubMed1983

[Poor fibrinolytic response to venous occlusion: defect of endothelial cells or plasma interference?].

To better characterize the plasminogen activators (v-PA) before and after venous occlusion, the euglobulins obtained from the plasma of 24 volunteers have been separated on SDS-PAGE and revealed zymographically. All the subjects with an excellent fibrinolytic response on fibrin plates (greater than 1.3 TAU/ml) exhibited activity of 68 kdaltons, which corresponds to free v-PA. The poor responders on fibrin plates (less than or equal to 0.3 TAU/ml) only showed increase of a lysis zone at 110 kdaltons, corresponding to a complex of v-PA and a fast-acting inhibitor. Hence poor fibrinolytic activity on fibrin plates after venous occlusion could be due to high plasma concentration of this inhibitor and not necessarily to defective production or release of v-PA.

Endothelium↗
PubMed1981

Relationship between plasma insulin levels and high density lipoprotein cholesterol levels in healthy men.

Insulin and high density lipoproteins are considered to play a role in the development of atherosclerosis. In order to study whether there was a relationship between endogenous plasma insulin response and high density lipoproteins, an acute intravenous glucose tolerance test (0.5 g glucose/kg body weight) was performed in 94 healthy men, aged 20-49 years. Cholesterol and triglyceride levels were measured in very low density lipoproteins, low density lipoproteins and high density lipoproteins isolated from fasting serum by preparative ultracentrifugation. The subjects were divided into quartiles according to their fasting and post-glucose load plasma insulin and high density lipoprotein cholesterol levels. The results obtained in the subjects of the upper quartiles were compared with the results obtained in the subjects of the lower quartiles. The mean glucose disappearance rates were within the normal range and did not differ between the upper and lower quartiles. Subjects with high fasting plasma insulin had lower high density lipoprotein cholesterol levels (1.11 +/- 0.34 mmol/l, p = 0.01) than men with low fasting plasma insulin (1.40 +/- 0.37 mmol/l). Higher mean post-glucose plasma insulin was associated with lower high density lipoprotein cholesterol levels (1.18 +/- 0.32 mmol/l, p less than 0.05) and increased high density lipoprotein triglyceride levels (0.14 +/- 0.07) mmol/l, p less than 0.01) when compared with the men with low post-glucose plasma insulin (1.40 +/- 0.36 mmol/l and 0.09 +/- 0.03 mmol/l respectively). These observations reflect the close relationship between endogenous insulin and lipoprotein metabolism.

Adult↗
PubMedDate not supplied

3D-Reconstruction of microglia and amyloid in APP23 transgenic mice: no evidence of intracellular amyloid.

Microglia cells are closely associated with compact amyloid plaques in Alzheimer's disease (AD) brains. Although activated microglia seem to play a central role in the pathogenesis of AD, mechanisms of microglial activation by beta-amyloid as well as the nature of interaction between amyloid and microglia remain poorly understood. We previously reported a close morphological association between activated microglia and congophilic amyloid plaques in the brains of APP23 transgenic mice at both the light and electron microscopic levels [25]. In the present study, we have further examined the structural relationship between microglia and amyloid deposits by using postembedding immunogold labeling, serial ultrathin sectioning, and 3-dimensional reconstruction. Although bundles of immunogold-labeled amyloid fibrils were completely engulfed by microglial cytoplasm on single sections, serial ultrathin sectioning and three-dimensional reconstruction revealed that these amyloid fibrils are connected to extracellular amyloid deposits. These data demonstrate that extracellular amyloid fibrils form a myriad of finger-like channels with the widely branched microglial cytoplasm. We conclude that in APP23 mice a role of microglia in amyloid phagocytosis and intracellular production of amyloid is unlikely.

Alzheimer Disease↗