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Biomedical subjects

M Sternberg

Publications and source records attributed to M Sternberg.

84 records · Page 5Linked to original sources
PubMed1969

Oxidation of isomaltose by Pseudomonas taetrolens.

Pseudomonas taetrolens NRRL B14 oxidized isomaltose without hydrolysis of the 1-6 glycosidic linkage. The resulting isomaltobionic acid was identified by chromatographic studies of acid hydrolysates and reconversion to isomaltose.

Acids↗
PubMedDate not supplied

The prevalence and nature of back pain in an industrial complex: a questionnaire and radiographic and HLA analysis.

A questionnaire relating to presence and nature of back pain was distributed to all 10,150 employees (59% men) of an industrial complex. The questionnaire was returned by 2892 subjects (65% men). Of these, 1880 (65% of responders or 19% of total) reported a history of back pain. One hundred twenty-four described their back pain as insidious in onset, persisting for at least three months, developing at less than 40 years of age, being associated with morning stiffness, and showing improvement with exercise. Three hundred sixty-seven subjects scored four of these five features. Pelvic radiographs of 342 persons were available for blind evaluation. Sixteen patients (12 men) were shown to have definite ankylosing spondylitis (Grade III or IV sacroiliitis or HLA B27-associated Grade II sacroiliitis). Only one of these persons was known to have spondylitis. The majority of these symptomatic patients had been seen by both medical and nonmedical practitioners.

Adolescent↗
PubMedDate not supplied

Expanding the donor pool--we need more living donors.

This paper describes some of the issues currently influencing thoughts and practice in expanding the use of living kidney donors. One of the challenges facing kidney transplantation is the increasing number of patients with Established Renal Failure (ERF) waiting for a kidney, and the ethical concerns raised as a result of meeting these challenges by the use of living donors. Nephrology professionals play an important role in the education of patients and potential living kidney donors and should be familiar with current issues related to this field.

Humans↗
PubMedDate not supplied

[Improving the interlaboratory variation for creatinine serum assay].

PURPOSE: To assess inter-assay variation and accuracy of blood creatinine measurements as well as the effect of the standardization of the calibration procedures on inter-assay variation. METHODS: Inter-assay variation and accuracy were assessed using 30 frozen human sera and 3 certified reference materials, which were analysed by 17 creatinine assays (colorimetric: 12, enzymatic: 4, HPLC: 1). Usual calibration procedure was compared with two common calibration procedures using either a reference material (404.1 micromol/L), or secondary sera calibrators (69, 115 et 180 micromol/L). RESULTS: Most of the commercially available methods display inaccuracy, > 10% for creatininemia < 150 micromol/L in most cases. For this concentration range, the mean creatininemia was statistically significantly different as a function of the assay used (p < 0.001). Enzymatic assays produced lower results than colorimetric ones for low creatinine levels but higher results for high creatinine levels. Assays being calibrated according to the manufacturer's recommendations, the median dispersion factor was 14% for the 20 samples between 45 and 150 micromol/L, and 8% for the 10 samples between 250 and 350 micromol/L. The calibration procedure modified inter-assay variation significantly (p < 0.001) but we gained little advantage from both common calibration procedures. A significant decrease of inter-assay variation occurred within each technical group (colorimetric or enzymatic) when a common calibration was performed using calibrators which concentration(s) was(were) close to the concentrations to be measured. CONCLUSIONS: Inter-assay variation is too high to allow prediction of glomerular filtration rate (GFR) or creatinine clearance from serum creatinine level. Our results highlight the interest of a calibration procedure using several concentrations with at least one between 90 and 150 micromol/L. The marketing of such a calibrator should be considered in order to decrease inter-assay variation in the range of creatinine levels which defines a mild chronic renal failure. Such an approach will certainly reduce inter-assay variation only within each technical group but could allow to include technical group as a co-variable in the algorithms developed for predicting GFR or creatinine clearance. A global transferability will certainly need the correlation of all types of creatinine assays versus a definitive method, whom definition remains uncertain.

Blood Chemical Analysis↗
PubMedDate not supplied

[Haptenic function and metabolism of the disaccharides of the glomerular basement membrane in heterologous nephrotoxic antibody-induced glomerulonephritis in rats].

The disaccharide unit Glc-gal-hyl represents 6% of dry weight of the rat glomerular basement membrane. It has been shown to be a haptenic determinant of this membrane. It appears to be one of the targets of the lesions induced by nephrotoxic antibodies in the rat: the urinary excretion of Glc-gal-hyl (as well as of Gal-hyl and Hyl which may derive from Glc-gal-hyl by degradation) during the autologous phase of the rat glomerulonephritis is significantly increased. Simultaneously the biosynthesis of Glc-gal-hyl, as measured by the glomerular glucosyltransferase activity, is elevated by 72%.

Animals↗