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Biomedical subjects

M Stier

Publications and source records attributed to M Stier.

At least 19 recordsLinked to original sources
PubMed1991

Single-step purification and biological activity of human nerve growth factor produced from insect cells.

Human nerve growth factor (NGF) was cloned and engineered for expression in a baculovirus-infected Spodoptera frugiperda (SF-9) insect cell system. Culture supernatants contained 2-3 mg/L of recombinant human NGF. The human NGF produced by this system was purified to apparent homogeneity with a single-step affinity chromatography procedure using a high-affinity monoclonal antibody originally raised against murine NGF. The purification procedure yielded 1-2 mg of pure, human NGF per liter of culture supernatant; i.e., approximately 60% recovery of the human NGF originally released into the culture medium. Although the gene transfected into the SF-9 cells coded for pro-NGF, the NGF recovered after purification was greater than 95% fully processed, mature protein. The KD for the affinity of the pure, recombinant human NGF for NGF receptor in PC12 membranes is 0.20 +/- 0.05 nM. Activation of neurite outgrowth in PC12 cells occurs with ED50 values of 85 +/- 20 pM and 9.6 +/- 1.5 pM for a 3-day primary response and a 1-day secondary response, respectively. The pure, recombinant human NGF also stimulates a significant increase in dopamine content of PC12 cells with an ED50 of 5.8 +/- 2.7 pM. These binding and biological activation properties are consistent with values observed using murine NGF purified from submaxillary glands.

Amino Acid Sequence↗
PubMed1977

[The thoracic radiographic picture in routine monitoring following unilateral allogenic lung transplantation in the dog].

Undesired effects of nonspecific immunosuppression may be delimited by individual dosage. Early recognition of allograft rejection is the presupposition for that steering. As long as immunologic test methods require a great expense of equipment, personnel, and time, and as other methods (bronchospirometry, lung puncture) after lung transplantation additionally endanger the recipient, clinical routine examinations are particularly important, primarly thorax x-ray examinations. The authors report on serial chest radiographs during the courses after 71 unilateral orthotopic allogenic pulmonary transplantations in dogs with regard to the findings at obduction. Possibilities of differential diagnosis shall be found by that, and the limits of interpreting x-ray films after lung transplantation shall be disclosed. Certain distinctive marks in the x-ray findings indicate the various possible complications; they can not be relied upon, however, when they only extend to the graft (hemorrhage, infection, rejection).

Animals↗
PubMed1976

[Experiences with computer-assisted x-ray diagnosis of pulmonary coin lesions (author's transl)].

Favourable results of the computer-aided diagnosis of peripheral bronchial carcinoma lead to a simplified scheme for calculation fo diagnosis, basing upon X-ray symptoms. This scheme was tested in the Zentralinstitut für Krebsforschung (ZIK) in 102 pateints and in the Forschungsinstitut für Lungenkrankheiten und Tuberkulose (FLT) in 101 patients. These patients had coin lesions of the lung with unknown diagnoses. The histological diagnoses later proved were compared with the calculated diagnoses. In ZIK there were correct diagnosis in 92.16% (in 96.53% of the carcinomas, in 44.4% of the tuberculomas and in all benign tumors), while in FLT 85.53% of the coin lesions were correctly classified (89.13% of carcinomas, 79.31% of the tuberculomas and all benign tumors). The comparison with the clinical-histological diagnoses showed a clear improvement of the accuracy by the calculated diagnoses. The method can be recommended for the basic diagnosis especially of peripheral bronchial carcinoma. It implies a reduction of the diagnostic delay, but it can not substitute the histologic investigation of the lesions.

Adult↗