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M Străuţ

Publications and source records attributed to M Străuţ.

6 recordsLinked to original sources
PubMedDate not supplied

Molecular subtyping of group A streptococcal strains based on virulence regulon polymorphism.

A total of 64 Streptococcus pyogenes isolates included in a previous study (Shundi et al., 2000) were further analyzed by Vir typing. Vir typing is based on restriction fragment length polymorphism (RFLP) analysis of a 4- to 8-kb pathogenicity island (vir regulon) encoding emm and other virulence genes. As all our isolates contained a single vir regulon, the stoichiometric yield of restriction fragments avoided ambiguities in interpretation of results. By using both HaeIII and HinfI restriction endonucleases to generate RFLP profiles, the 64 GAS isolates were distributed among 22 Hae- and 26 Hinf-Vir types respectively.

Bacterial Proteins↗
PubMedDate not supplied

Correlation between two DNA-based typing methods applied to Streptococcus pneumoniae strains: BOX-PCR assay as an alternative to ribotyping.

Seventy-nine Streptococcus pneumoniae isolates were characterized by ribotyping and BOX-PCR typing. The isolates comprised 14 most frequently identified serogroups and serotypes, 54 ribotypes (defined as each different combination of two DNA restriction fragment length polymorphism patterns obtained with HindIII and PvuII, respectively) and 51 BoxA-PCR patterns. There was close but not absolute correlation between molecular techniques. However, the discrimination indice of ribotyping--calculated on the basis of combination of the data obtained with both enzymes--equals that of BOX-PCR typing: D = 0.985 and the deduced genetic clustering of the pneumococcal strains was comparable.

Bacterial Typing Techniques↗
PubMedDate not supplied

Antibiotics and bacterial resistance. A few elements of genetic basis for this relationship.

In the preantibiotic era, many people died of bacterial infections caused by such pathogens as Staphylococcus aureus and Streptococcus pyogenes, Streptococcus pneumoniae and Mycobacterium tuberculosis. Antibiotics have reduced the mortality from infectious diseases but not the prevalence of these diseases. It was not long after the clinical introduction of the first antibiotics in the 1950s that the first reports of bacterial resistance began to appear. Use, and often abuse or misuse, of antimicrobial agents has encouraged the evolution of bacteria toward resistance, resulting often in therapeutic failure. In the beginning, new antibiotics have always appeared in plenty of time to provide new cures for diseases caused by resistant bacterial pathogens. Also, some clinically important groups of bacteria showed no signs of major increases in resistance. For example, S. pneumoniae strains remained susceptible to penicillin long after other bacteria had become resistant to it. Recent developments of bacteria resistance to antibiotics are indeed disquieting.

Bacterial Infections↗