PubMed Health⌕ Search

Biomedical subjects

M T Bassoullet

Publications and source records attributed to M T Bassoullet.

3 recordsLinked to original sources
PubMed1995

Stealth Me.PEG-PLA nanoparticles avoid uptake by the mononuclear phagocytes system.

Nanoparticles were prepared from methoxy poly(ethylene glycol)poly(d,l-lactic acid) block copolymers (Me.PEG-PLA) or blends of Me.PEG-PLA and PLA by the precipitation-solvent diffusion method. These nanoparticles, labeled by introducing [14C]PLA in the formulation, were shown to be more slowly captured by cultured THP-1 monocytes than F68-coated PLA nanoparticles, in a PEG chain-length-dependent manner. In vivo, the half-life in plasma of the Me.PEG-PLA nanoparticles that were intravenously administered to rats is increased by a factor 180 compared with the F68-coated PLA nanoparticles. This mononuclear phagocytes system avoidance was explained according to a conformation model in which the PEG density at the surface of the particles is a key parameter.

Animals↗
PubMed1986

Effects of pefloxacin on phagocytosis function of rat macrophages and polymorphonuclear leucocytes.

The influence of pefloxacin on polymorphonuclear leucocytes (PMN) and alveolar macrophages (AM) phagocytosis and microbicidal activity was studied. Phagocytes were collected from Sprague-Dawley rats, mixed with pefloxacin at different concentrations (1, 10, 100 mg/l) and a yeast phagocytosis assay was performed. Three parameters were measured, the phagocytic capacity (number of live and dead yeast/100 cells), the phagocytic activity (number of cells containing 2 or more yeast/100 cells) and the microbicidal activity or % killed (Formula: see text) Both low and high concentrations of pefloxacin increased significantly the phagocytic capacity and activity of AM and PMN. The increase of the AM phagocytic capacity and activity seen with increasing levels of extracellular pefloxacin was not statistically significant. Pefloxacin showed no adverse effect on the microbicidal activity of AM and PMN.

Animals↗