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Biomedical subjects

M T Bastos

Publications and source records attributed to M T Bastos.

9 recordsLinked to original sources

Tensile strength of five types of retention for resin-bonded prostheses.

The tensile bond strengths of five retention techniques for bonded cast restorations were tested. The retentive methods were the electrolytic etch, Rochette's perforations, retention beads, the recessed surface, and soldered orthodontic mesh. The electrolytic etch and recessed surface methods gave the highest values of bond strength, followed by retention beads. Rochette's perforations and the soldered orthodontic mesh failed to surpass the bond strength of the control group (no additional retention) after 1 hour and provided the lowest bond strength after 24 hours.

Acid Etching, Dental↗

Methotrexate: studies on cellular metabolism. IV. Effect on the mitochondrial oxidation of cytosolic-reducing equivalents in HeLa cells.

The effect of methotrexate (MTX) on the mitochondrial oxidation of cytosolic-reducing equivalents in HeLa cells was studied. MTX inhibited (100 per cent) malate dehydrogenase activity, but no effect was observed on that of GOT. MTX (0.5 mM) inhibited (100 per cent) the activity of reconstituted enzymatic system MDH-GOT, probably as a consequence of inhibition of malate dehydrogenase activity. MTX decreased pyruvate production (54 per cent), demonstrating its inhibitory action on the malate-aspartate shuttle. Blockage of the malate-aspartate shuttle by MTX accounts for the decrease in cellular energetic gain. The results obtained are consistent with the view that in HeLa cells, as well as in other tumour cells, the transport of reducing equivalents from cytoplasmic NADH into the respiratory chain of mitochondria is via the malate-aspartate shuttle.

Arsenic↗