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Biomedical subjects

M T Bomar

Publications and source records attributed to M T Bomar.

9 recordsLinked to original sources

Assay of the inhibitory effect of pentachlorophenol and formaldehyde on mycelial growth and development of reproductive structures in Aspergillus niger.

The biological activity of the antifungal agents pentachlorophenol (as sodium pentachlorophenolate) and formaldehyde was evaluated by changes in the development of fungal structures following a placement of test strips inoculated with Aspergillus niger conidia on a nutrient agar with the toxicant. The method allows a quantitative assessment of biological activity measured as the development of vegetative structures (growth of the mycelium) by metric, and the development of reproductive structures (conidia) of A. niger by densitometric methods. The use of test strips with dry conidiospores and the evaluation of the results in physical units represent a simple, rapid, exact and inexpensive test of fungitoxic agents.

Aspergillus niger↗

Influence of surface chemistry of the substrate on the adsorption of Escherichia coli.

The adsorption of Escherichia coli as influenced by the surface chemistry of different absorbents was studied with microbeads of (1) polystyrene, hydroxymethylated, chloromethylated and aminomethylated polystyrene, (2) cation and anion exchange resins, (3) glass and siliconized glass using a shake test. The adsorption kinetics can be described by a first order rate law for all materials. The specific surface free energy is not even a rough correlation parameter for the adsorption effect. Polar groups with the exception of those with a negative charge (cation exchange resin) favour adsorption of Escherichia coli, but the type of the polar surface group plays a dominating role. Adsorption was maximum with protonated aminomethylated polystyrene which is attributed to the interaction of the positive charge with the negatively charged bacterial surface.

Bacterial Adhesion↗

Bacterial growth in artificial capillary spaces.

In experiments on the influence of microscopic capillaries on the growth of Escherichia coli, Brevibacterium linens, Micrococcus flavus and Bacillus cereus, a distinct delay in growth was observed. The difference in counts was greatest after 8 h. Later a slight equilibration of counts was noted. With B. cereus, only slight or no spore formation was observed under microcapillary conditions.

Bacillus cereus↗

[Effect of solid substrates of microbial colony development].

Bacteria have been found to grow differently at contact sites to both organic and anorganic solids as on pure agar media. Of 16 ad hoc selected bacterial strains, the following species (arranged in decreasing order) responded with stronger colony formation: Bacillus subtilis, B. cereus, Streptococcus sanguis, S. saprophyticus, S. faecalis, Escherichia coli, Micrococcus flavus, Actinomyces viscosus, Veillonella sp., Enterobacter liquefaciens, Yersinia enterocolitica. Lactobacillus casei behaved neutral. Proteus vulgaris, Y. enterocolitica and Chromobacterium violaceum grow on certain media only in the presence of solid particles. The yeasts Endomyces lactis, Saccharomyces cerevisiae, Rhodotorula mucilaginosa and Cryptococcus nigricans did not respond to solid particles during colony formation. The kind of protein-animal (meat) or vegetable (soy beans)-did not influence the behaviour of the microorganisms in these experiments.

Agar↗

[Bacteria and dietary fiber].

The presence of cellulose particles, so in-vitro studies have shown, influences the growth rate and metabolic activity of the bacteria Escherichia coli, Bifidobacterium adolescentis and Chromobacterium violaceum. After the homogenization in Waring Blendor, the growth rate has been found to be highest in a medium containing 10% cellulose particles and to show a decreasing tendency in media containing 5%, 1% and 0% cellulose. The biochemical intensity, judged by glucose consumption and intensity of nitrate reduction, showed an inverse tendency. It seems necessary to add also the factor "biochemical activity" of the microorganisms to the effect of dietary fibres in the intestinal tract.

Bifidobacterium↗

[Bacteriological aspects of holding freshly prepared spinach at warm temperatures. Relation between bacterial count and nitrate reduction].

In sterilized spinach which was inoculated with bacteria, a reduction of NO3- to NO2-, due to their metabolic activity, was detectable, depending on the holding conditions. The reaction rate was highest in the presence of thermophilic bacteria. A lower reaction rate was altogether found with mesophilic bacteria which, depending on counts and holding time, may temporarily cause high nitrite contents. The reaction rate was lowest with cold-tolerant bacteria.

Food Microbiology↗

[Microbiological aspects of stored hard-boiled eggs].

Hard-bioled eggs in the shell were without any pretreatment stored in air at 20 degrees C and 4 degrees C. Furthermore the keeping quality of varnish-coated eggs stored in air at 20 degrees C and of untreated eggs stored in 100% carbon dioxide at the same temperature was studied. Hard-boiled eggs stored at 4 degrees C and varnish-coated eggs stored at 20 degrees C were of excellent bacteriological quality up to 5 weeks. Untreated eggs stored in air at 20 degrees C showed high microbiological contamination (more than 10(6)/g) already after one week. The CO2 storage atmosphere inhibited the growth of microorganisms during the first week of storage, but in the days to follow counts increased to more than 10(6)/g as well.

Aeromonas↗