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Biomedical subjects

M T Daniel

Publications and source records attributed to M T Daniel.

13 recordsLinked to original sources
PubMed1979

[A new variety of acute non-promyelocytic leukemia with t(15;17)].

Three cases of a new variety of acute leukemia have been reported. The main features were: hyperleukocytosis made of large-sized blasts with a double shaped nucleus, few or no granulations in the cytoplasm, and in a few cell faggots or unique Auer rods; mycloperoxydase reaction was positive. This feature was associated with disseminated intravascular coagulation syndrome and t(15;17)(q22;q21) translocation in the majority of mitoses.

Acute Disease↗
PubMed1979

Leukemic phase of malignant histiocytosis (arguments in favour of the histiomonocytic origin of the abnormal cells).

A 15-year-old female was treated for malignant histiocytosis. The occurrence of a leukemic phase (178,000 blasts/cu mm) during the follow up provided the opportunity of studying a large number of malignant cells by cytochemical electron microscopic, and cell membrane markers techniques. Acid phosphatase reaction was strongly positive and totally inhibited by tartaric acid. Nonspecific esterase reaction was moderately positive with inhibition by sodium fluorid. Electron microscopy revealed the presence of surface membrane pseudopods and the phagocytic activity of the cells. The leukemic cells had a receptor for the Fc fragment of IgG. These findings support the histiomonocytic origin of the abnormal cells in malignant histiocytosis.

Adolescent↗
PubMed1979

A new translocation in Burkitt's tumor cells.

A t(8;22)(q24;q11) translocation was found in blood, bone marrow, and ascites cells from a European Burkitt's lymphoma. Cell surface markers were identified as monoclonal IgG. The relationship between these two unusual findings is questionable in this cytologically typical Burkitt's lymphoma.

Aged↗
PubMed1979

[T (15;17) translocation in acute promyelocytic and acute nonpromyelocytic leukemia (author's transl)].

Seven acute promyelocytic leukemias (APL) were compared with three atypical acute myeloblastic leukemias (AML). These three AML were characterized by high hyperleukocytosis, mostly formed of monocytelike myeloblasts, disseminated intravascular coagulation syndrome, and a t (15;17) translocation in the majority of leukemic cell mitoses. This translocation was inconsistently found in typical APL defined as M3, according to the FAB classification.

Adolescent↗
PubMed1978

[Use of the LARC system in a laboratory specializing in hematology (author's transl)].

Two thousand consecutive white blood cell counts were simultaneously studied by the LARC system and the traditional manual method. This comparison enables the following four couclusions to be drawn.--The reproducibility of the LARC differential is superior to the traditional method;--The similarity between results obtained by the LARC and the manual method are good, as indicated by scatter-plots and calculated correlation coefficients;--The LARC system can be substituted for the traditional manual method, also for markedly pathologic samples, but in this case at the price of a slowing down of the through-put rate of the system;--The detection of abnormal white cell types is as good or better with the LARC system as compared with the manual method.

Autoanalysis↗
PubMed1977

Surface features of Sezary cells: A scanning electron microscopy study of 5 cases.

The surface features of circulating cells from 5 patients with typical Sezary's Syndrome (SS) are described using scanning electron microscopy (SEM). Sezary cells prepared by different methods, with and without prior fixation in cell suspension, showed similar surface architectures. SS cells were mostly spherical and moderate to markedly villous in appearance, and in this respect, resembled the majority of circulating lymphocytes from patients with chronic lymphocytic leukaemia (CLL). A proportion of cells were larger and more irregular in shape while others had small extensions of cytoplasm resembling small uropods with clusters of polarised microvilli. Despite the latter findings, most SS cells cannot be distinguished from CLL cells on the basis of their surface architecture under the SEM.

Cell Membrane↗
PubMed1976

Proposals for the classification of the acute leukaemias. French-American-British (FAB) co-operative group.

A uniform system of classification and nomenclature of the acute leukaemias, at present lacking, should permit more accurate recording of the distribution of cases entered into clinical trials, and could provide a reference standard when newly developed cell-surface markers believed to characterize specific cell types are applied to cases of acute leukaemia. Proposals based on conventional morphological and cytochemical methods are offered following the study of peripheral blood and bone-marrow films from some 200 cases of acute leukaemia by a group of seven French, American and British haematologists. The slides were examined first independently, and then by the group working together. Two groups of acute leukaemia, 'lymphoblastic' and myeloid are further subdivided into three and six groups. Dysmyelopoietic syndromes that may be confused with acute myeloid leukaemia are also considered. Photomicrographs of each of the named conditions are presented.

Acute Disease↗
PubMed1975

Septate-like junctions in abnormal erythroblasts: cytochemical, ultrastructural and freeze-etch studies.

Ultrastructural studies of the bone marrow of a patient with refractory anemia revealed aberrant erythroblasts with unique cell junctions. Periodic structures linked adjacent processes on the same cell as well as surfaces of neighboring erythroblasts. Inclusions circumscribed by similar complexes were also present in the cytoplasm. The junction appeared in cross sections as two rigidly parallel unit membranes separated by 250-300 A interspace with two regular arrays of facing 70 A particles at intervals of 160-200 A. These intracellular specializations were present between erythroblasts at various stages of maturation and between, mono, bi or multinucleated erythroblasts. Junctions were permeable to lanthanum and many that appeared to be intracellular were demonstrated to be continuous with the extracellular space. The fact that others were not penetrated by lanthanum indicates that detachment of the interdigitating processes from cells of origin could have occurred. In freeze-etched replicas, distribution of membrane particles was random in areas of septate-like junctions, although parallel rows were sometimes observed both on A and B inner hydrophobic faces of the membrane leaflets. Junctional complexes in tissue culture appeared to have been disrupted and were not reestablished; however, inclusions resembling internalized junctions were observed associated with multivesicular bodies. Ineffective erythropoiesis and the resulting refractory anemia appear to be associated with the presence of the described anomalous junctional complexes.

Anemia, Aplastic↗
PubMed1975

Acute leukemia with Burkitt's tumor cells: A study of six cases with special reference to lymphocyte surface markers.

In six patients with acute leukemia (about 2% of the patients referred for acute lymphoblastic leukemia) the blast cells invading bone marrow and blood showed all the cytologic, cytochemical, and electron microscopy features of Burkitt's tumor cells. The presence of monoclonal surface immunoglobulins (their synthesis being proved by in vitro culture experiments), the binding of IgG aggregates, and the absence of rosette formation with sheep red cells documented the monoclonal B-cell origin of these blast cells which is in sharp contrast to the findings in common acute lymphoblastic leukemia. The course of the disease was usually rapidly fatal without chemotherapy-induced remission.

Acid Phosphatase↗
PubMedDate not supplied

[Acute mast-cell leukemia. Cytochemical and ultrastructural study, about a particular case (author's transl)].

A case of acute Mast-cell leukemia was studied. A 39 years old female presenting with a brief history of abdominal pain and attacks of flushing; peripheral blood and bone marrow contained up to 60% of poorly differentiated blasts with clumping of deep purpule granules. Peroxydase reaction stains were negative, chloroacetate esterase were strongly positive. Toluidine blue revealed metachromatic stain. Histamine content of the cells was highly greater than normal but nos heparinoid activity could be demonstrated. These abnormal mast-cells have been investigated with the electron microscope; only the dense particular type of granule substructure was found, without any lamellae component. The cells were temptatively classified as "immature" mast-cell. The disease was interpretated as an acute leukemic variety of systemic mastocytosis.

Acute Disease↗