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M T Graziani

Publications and source records attributed to M T Graziani.

4 recordsLinked to original sources
PubMed1990

Biochemical and immunological studies of deglycosylated Rhus vernicifera laccase.

Rhus vernicifera laccase (Japanese) was deglycosylated by treating it with exo- and endoglycosidases. In absence of unfolding agents deglycosylation does not exceed 32% while in denaturating conditions only 10% of saccharides are not digested. In the former case specific activity seems to be directly related to the amount of the residual carbohydrates. After deglycosylation in denaturing conditions the modified enzyme exhibits an electrophoretic component of about 60-65 Kd. A stabilizing effect of saccharide moiety on the catalytic site of native laccase was demonstrated. Antiserum raised against the enzyme recognized native Japanese, Vietnamese as well as deglycosylated laccase indicating that the two isoforms are immunologically similar.

Antigen-Antibody Complex↗
PubMed1977

Pulsed electron paramagnetic resonance studies of types I and II coper of Rhus vernicifera laccase and porcine ceruloplasmin.

Electron spin-echo decay envelopes for types I and II copper of Rhus vernicifera laccase and for type II copper of procine ceruloplasmin have been studied. Nuclear modulation patterns show that imidazole is a ligand for all of them. The linear electric field effect (LEFE) in EPR was studied for type I copper in a laccase preparation from which type II had been removed. The symmetry of the site is near tetrahedral and the magnitude of the LEFE is correlated with the intensity of blue color.

Animals↗