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M T Klein

Publications and source records attributed to M T Klein.

14 recordsLinked to original sources
PubMed2003

Serological studies of monoclonal RH antibodies with RH1 (D), RH2 (C), RH3 (E) and RH5 (e) variant RBCs.

One hundred and forty five Mabs against RH antigens were tested. In this paper, we chose to detail reactivity of MoAbs directed against variant RBCs of the CNRGS collection for which we studied the molecular background. Because we developed procedures to identify variants of the RhD, RhC, RhE and Rhe antigens, we were especially interested in finding new monoclonal antibodies that could help us to characterize more accurately these variants. Therefore, we drew parallels between our procedures and results obtained with the 2001 workshop antibodies.

Antibodies, Monoclonal↗
PubMed1996

Serological studies of monoclonal RH1(D) antibodies with RH1(D) variants.

In this paper we chose to emphasize three aspects of our work. First we underlined that "low grade and high grade" D weak red blood cells studied at the DNA level could, when monoclonal antibodies were used, give patterns of positive and negative reactions like partial RH1(D) cells. Secondly, we showed the importance of the technical conditions of the study which are essential for establishing a pattern of reactivity defining an epitope. It appears that the use of papain treated cells at room temperature can be misleading for the definition of epitope especially with IgM antibodies. Lastly we pointed out the interest of Rh variant cells, defined at the gene level, to study the expression of RH1(D) epitopes on the external part of the membrane.

Antibodies, Monoclonal↗
PubMed1989

[The erythrocyte phenotype Rh: 32,-46: transfusional and obstetric importance].

The red blood cells and sera from 21 RN/RN individuals were studied. The study confirmed the Rh type D+C+E-c-e+, CW-, characterized by an increased expression of the D antigen, a markedly decreased expression of the C and e antigens, the presence of a low incidence antigen (Rh32) and the absence of a high incidence antigen (Rh46) associated with an epitope recognized by a murine monoclonal antibody (MR 432). Four individuals exposed to Rh46 cells by pregnancy and/or transfusion had an anti-Rh46 antibody. This antibody gave positive reactions with all red blood cells of common and rare Rh phenotype except Rh null, D--, D.., DC(W-) and RN/RN cells. This antibody is considered to be of clinical significance in case of transfusion or pregnancy.

Africa↗
PubMed1987

Study of anti-Fya in five black Fy(a-b-) patients.

Fya antibodies were detected in 5 Fy(a-b-) black patients during a 5-year period. The Duffy phenotype was confirmed using anti-Fya, anti-Fyb, and anti-Fy3 by an adsorption-elution technique. These 5 patients had sickle cell disease and had received multiple blood transfusions. The antibodies were all found during the investigation of a hemolytic transfusion reaction or failure to achieve the desired red cell increments after transfusion.

Adult↗
PubMed1985

Anaphylaxis due to suxamethonium? A case report.

A case of profound cardiovascular collapse after the administration of Althesin (alphaxalone-alphadolone) and suxamethonium is described. During subsequent anaesthesia in the same patient, cardiovascular collapse followed the administration of thiopentone and suxamethonium. It is concluded that suxamethonium, rather than Althesin, was the provocative agent.

Adult↗
PubMed1985

Ventilation monitors and alarms.

In this review we present a proposed classification of the various types of ventilation monitors and failure alarms used in anaesthetic practice. Each monitoring method is then discussed individually and the advantages and disadvantages are evaluated.

Anesthesia↗
PubMed1981

An evaluation of the Samson and Magill (Mapleson A) circuits during anaesthesia.

The valveless scavenging circuit of Samson was compared with a standard Magill (Mapleson A) circuit. Replacement of the scavenging unit of the Samson circuit by the Heidbrink valve of the Magill circuit at a given fresh gas flow rate allowed each patient to act as his own control. The minimum and maximum carbon dioxide levels were estimated at varying fresh gas flows related to the body weight of the patient. The carbon dioxide elimination of the two circuits showed no statistical difference and it is concluded that the Samson circuit requires fresh gas flows similar to the Magill circuit. The Samson circuit scavenged adequately during spontaneous breathing, manual ventilation and even when using the emergency oxygen bypass.

Adult↗
PubMedDate not supplied

Emm. A red cell antigen of very high frequency.

Anti-Emm, an antibody to a public red cell antigen, was found in the serums of four Emm-propositi and in the serum of the Emm-brother of one of them. Three of the propositi were men, and their antibodies, as well as that of the compatible brother, were believed to be "naturally occurring." Since there are two Emm-siblings in one family, Emm is probably an inherited characteristic.

Adult↗
PubMedDate not supplied

A serologic study of red cells and sera from 18 Rh:32,-46 (RN/RN) persons.

The red cells (RBCs) and sera from 18 RN/RN persons were studied. The study confirmed the Rh type D+C+E-c-e+Cw-, which is characterized by an increased expression of the D antigen; a markedly decreased expression of the C and e antigens; the presence of a low-incidence antigen, Rh32; and the absence of a high-incidence antigen, Rh46, which is associated with an epitope recognized by a murine monoclonal antibody (MR432). Other Rh antigens of low and high incidence were investigated, and the presence of Rh17 and Rh44 on the RBCs was confirmed. Three persons exposed to Rh:46 cells by pregnancy or transfusion (or both) had anti-Rh46. This antibody gave positive reactions with all RBCs of common and rare Rh phenotype except Rhnull, and those of D--, D.., DCw-, and RN homozygotes. This antibody is considered to be of clinical significance in case of transfusion or pregnancy.

Adult↗