Biomedical subjects

M T Tarkka

Publications and source records attributed to M T Tarkka.

At least 19 recordsLinked to original sources

PubMed2001

Teacher candidates' reflective teaching and learning in a hospital setting - changing the pattern of practical training: a challenge to growing into teacherhood.

UNLABELLED: AIMS OF THE PROJECT: The main goal of the 'teacher candidates' reflective teaching and learning' project was to integrate theories of reflection in education and nursing practice. This was realised through teacher candidates' practical training in a hospital setting providing them with personal experiences of reflection in different forms and contexts. In this paper one teacher candidate pair's experiences of developing documentation in one operating theatre are described. BACKGROUND: The power of reflection is acknowledged in health care education and, on the other hand, also in nursing practice. Reflection, however, is not spontaneous but it requires active contribution. The key persons in this respect seem to be healthcare teachers. The challenges for today are that, for example, the theoretical background of reflection is multi-fold and that the concept of reflection is popularised in common use. DESIGN OF THE PROJECT: The project (1995-1998) was accomplished in one of the biggest university hospitals in Finland. On the collaborating wards, systematic team supervision was in progress. Teacher candidates planned and implemented in-service-training modules for teams based on educational needs raised during team supervision sessions. FINDINGS: The contribution of the project can be assessed as important to health care teacher education, but also to nursing practice. Practical training in a hospital setting enhanced teacher candidates' ability to work as teachers in a hospital organisation as well as a nursing college/polytechnic by providing them with a new viewpoint. They learned valuable lessons from applying theory, as planning in-service-training required continuous reflective discussion with the nursing staff but also theoretical reflection. Improvements in nursing practice were also promoted as staff educational needs were met. CONCLUSIONS: Shifting the teacher candidates' practical training into a hospital setting and emphasising reflection seemed to promote their professional development towards teacherhood and improve nursing practice.

Adult↗

PubMed2001

Gender differences and fears in patients awaiting coronary artery bypass grafting.

The study aimed at ascertaining the gender differences and fears among patients awaiting coronary artery bypass grafting. Data were collected using a mailed questionnaire from patients (n=207) scheduled for coronary artery bypass grafting at one hospital. Intensity of fear was measured on a 10-point scale including 12 objects of fear. The results showed that demographic data, lifestyle and functional capacity differed between the sexes. Patients' fears differed with respect to their objects and intensity, with women reporting more intense fears. Men also had fears, but the objects of fear differed from those in women. Apart from gender, the intensity of fear was associated with physical exercise, emotional problems and depression. Nursing interventions could be developed to meet the different needs for information and support, especially among women but also among men, to relieve their fears during the wait for coronary artery bypass grafting.

Activities of Daily Living↗

PubMed2001

Scots pine expresses short-root-specific peroxidases during development.

Nine short-root-specific proteins from Scots pine (Pinus sylvestris L.) detected and isolated as individual spots by 2D-PAGE were identified. The similar peptide mass maps obtained for all nine polypeptide spots together with lectin-blotting results suggest that they represent forms of the same modified protein. N-Terminal sequence analysis of two of the peptides showed high similarity to peroxidases. RT-PCR with oligonucleotide primers corresponding to determined peptide sequences and conserved regions in plant peroxidases led to isolation of Psyp1 cDNA which is most abundantly expressed in short roots. Psyp1 is the first peroxidase cDNA to be isolated from the genus Pinus. It encodes a 363-amino-acid class-III peroxidase with a calculated molecular mass of 35.7 kDa and theoretical pI of 4.74. The predicted PSYP1 amino-acid sequence is grouped with other class-III peroxidases in phylogenetic analyses, but it has a unique amino-acid sequence which may be associated with its function in short roots or with its phylogenetic group. The presence of a signal sequence for extracellular transport indicates that PSYP1 belongs to the group of secreted class-III peroxidases. The presence of 10 tyrosine residues and putative auxin-binding regions in PSYP1 suggests that the function of the enzyme is associated with cell-wall formation in short roots. The downregulation of Psyp1 expression in symbiotic short roots hosting the ectomycorrhizal fungus Suillus bovinus is perhaps related to the change in cell-wall structure necessary for ectomycorrhizal development.

Amino Acid Sequence↗

PubMed2001

Characterization of small GTPases Cdc42 and Rac and the relationship between Cdc42 and actin cytoskeleton in vegetative and ectomycorrhizal hyphae of Suillus bovinus.

This work reports the isolation and molecular characterization of CDC42 and RAC1 cDNAs from the ectomycorrhiza forming filamentous homobasidiomycete Suillus bovinus. Previously, no RAC gene was described from filamentous fungi and no CDC42 gene was described from homobasidiomycetes. Southern hybridization with SbCDC42 and SbRAC1 cDNAs indicated that the S. bovinus genome contains only one CDC42 and one RAC1 gene. The predicted amino acid sequence of SbRaclp is 77% identical with the Rac1B protein of chick, whereas SbCdc42p is most identical with Schizosaccharomyces pombe Cdc42p, showing 88% identity. In the predicted amino acid sequences of SbRaclp and SbCdc42p, the five guanine nucleotide binding regions, switch I and II, and the effector domain are highly identical to those known in other small GTPases. These domain structures suggest that in S. bovinus, SbRac1p and SbCdc42p function as molecular switches regulating the organization of actin cytoskeleton, similar to yeasts and mammals. SbRAC1 and SbCDC42 were expressed in vegetative and ectomycorrhizal hyphae, and SbCdc42p was detected in ectomycorrhiza-forming hyphae if growth and differentiation of the symbiotic hyphae took place. Cdc42p and actin were localized at the tips of S. bovinus vegetative hyphae. Similar to yeast, in filamentous fungi Cdc42p may be necessary to maintain the actin cytoskeleton at hyphal tips, making the polarized growth of the hyphae possible. In developing ectomycorrhiza, Cdc42p and actin were visualized in association with plasma membrane in swollen cells typical to the symbiotic hyphae. The role of Cdc42p and actin in regulation of the growth pattern and morphogenesis of ectomycorrhizal hyphae is discussed.

Actins↗

PubMed2000

Molecular characterization of actin genes from homobasidiomycetes: two different actin genes from Schizophyllum commune and Suillus bovinus.

The actin-encoding genes Scact1 and Scact2 of the homobasidiomycete Schizophyllum commune are the first actin genes isolated from higher filamentous fungi. Their isolation shows that homobasidiomycetes have two actin encoding genes instead of one typical to yeasts and filamentous ascomycetes. This result was further confirmed by cloning two actin encoding genes, Sbact1 and Sbact2, from another homobasidiomycete Suillus bovinus. The comparison of the genomic and cDNA sequences of the actin genes showed that Scact1 and Scact2 genes of S. commune contain seven introns, five of which are at the same position in the two genes while S. bovinus actin genes contain nine similarly positioned introns. In the four genes, five intron positions are shared, which indicates a close relationship between the actin encoding genes from S. commune and S. bovinus. Northern hybridization and analysis of two-dimensional immunoblots showed a difference in the expression levels between the two actin genes in each fungus. No actin protein could be detected from S. commune Scact2. The deduced amino acid sequence of the Scact2 gene also differs considerably from any other known actin protein. These data suggest that the Scact2 gene either has a special as yet unidentified function in S. commune life cycle or is a transcribed but no longer translated pseudogene. Scact2 gene has a putative microORF (short open reading frame) and Scact1 gene an intron in the 5'-untranslated region, which could reduce the translational efficiency and increase the transcriptional efficiency of the Scact2 and Scact1 genes, respectively. During mating in S. commune or at formation of ectomycorrhiza in S. bovinus, the expression of actin genes was similar to that in vegetative hyphae. This result suggests that the reorganization of actin cytoskeleton in response to extra- and intracellular signals in higher filamentous fungi could be directly regulated by members of signalling pathways well characterized in yeast and mammalian cells.

Actins↗

PubMed2000

First-time mothers and child care when the child is 8 months old.

The aim of the present study was to examine those resources and strengths that mothers find helpful for coping with child care when the child was 8 months old. This study is part of a more extensive longitudinal study project in which the growth into motherhood of first-time mothers was followed for 8 months postpartum. Data were collected by using structured questionnaires between August 1995 and March 1996. The sample comprised 254 first-time mothers and 248 mothers returned the questionnaires by mail. The multivariate method used was a stepwise regression analysis. Predictors included in the multivariate method to explain coping with child care were as follows: the mother's competence, mother's attachment to the child, mother's self-concept, relation to the spouse, breastfeeding, decision-making support from the public health nurse and activity of the child. The strongest predictor was competence as a mother. The more competent the mother felt and the more attached the mother was to her child, the better her coping. The better the mother's self-concept and relationship with the spouse were, the better she succeeded in taking care of the child. If the mother still breastfed her child and received decision-making support from public health nurses, she coped better in child care. Finally the more active the child was, the more the risk for unsuccessful child care increased. The results indicate that the first-time mother's successful coping with child care when the child is 8 months old is associated with her own resources and attachment to the child as well as activity of the child and breastfeeding. A good relationship between the spouses and support for decision making from the public health nurse also contribute to coping with child care.

Adaptation, Psychological↗

PubMed2000

Peer evaluation in nurses' professional development: a pilot study to investigate the issues.

Peer evaluation in nursing is a method by which the nurse evaluates the work of a peer, according to set evaluation criteria. The aim of the study was to clarify the potential significance of peer evaluation with regard to nurses' career development and relates to the introduction of a career development programme for nurses in a Finnish University Hospital. The research concepts were created on the basis of literature analysis. The concepts served as a basis for data collection, and five open-ended questions were devised from them. Informants (n = 24) gave free-form essay-type answers to these questions. The material was analysed using qualitative content analysis. The results indicate that self-evaluation constitutes the basis for peer evaluation. Peer evaluation allows nurses to give and receive professional and personal support promoting professional development. Professional support offers possibilities for change and alternative action. Personal support requires respect for the peer's equality and individuality. Personal peer support can decrease feelings of uncertainty and insecurity caused by work. The conclusion is drawn that peer evaluation is a means of promoting nurses' professional development to further on-the-job learning in collaboration with peers.

Adult↗

PubMed2000

How first-time mothers cope with child care while still in the maternity ward.

The aim of the study was to ascertain those factors that are related to the way first-time mothers assess themselves to be coping with child care when leaving the maternity hospital. The study is part of a wider longitudinal project that follows-up first-time mothers' growth into motherhood for a period of 8 months after the birth. Data collection was done using a questionnaire distributed between January and May 1995. The sample comprised 271 first time mothers. The mothers completed the questionnaire on average on the fifth day after childbirth. A stepwise regression analysis was applied. The mothers who assessed themselves to be coping better with child care were those who went home feeling rested and in a good frame of mind and who had positive experience of child care in the maternity ward. The more functional support the mother perceived herself to receive from members of her social network the better she assessed herself to be coping with child care. Those mothers who had previous experience of taking care of infants also assessed themselves to be coping better with child care. On the basis of the research findings it may be stated that the first-time mother needs support from both nursing professionals and close relatives in order to trust their own abilities to cope better with child care during early motherhood.

Adaptation, Psychological↗

PubMed2000

The staff's experience of the death of a child and of supporting the family.

The purpose of the study was to describe how staff members experience the death of a child and how they support the grieving family. The goal is to find means to improve supporting activities in the university hospital. The data for this study were collected with a questionnaire given to the staff who encounter the death of a foetus or infant at their work (n = 102). The questionnaire contained both structured and open-ended questions. Altogether, 69 members of the staff (68%) answered the questionnaire. The open-ended questions were analysed using content analysis. The staff experiences of a child's death manifested themselves in grief, feelings of injustice, experiences of one's limited resources, and relief. About half (51%) of the respondents estimated that they were able to support families rather well. Education in supporting the grieving family increased the ability to support families. Social support for families included emotional, informational, and concrete support. The majority of the staff expected support from colleagues after the death of a child. The study results show that the hospital staff supports the grieving family, but also recognizes their inadequacy as supporters. The staff also needs support in order to be able to support the grieving family.

Adaptation, Psychological↗

PubMed2000

Oncology ward nurses' perspectives of family grief and a supportive telephone call after the death of a significant other.

To improve family nursing in oncology wards, a new nursing intervention was created: a supportive telephone call after the death of the patient. Nurses who participated in the intervention kept diaries after the call (n = 95). Data were collected also from hospital records. The numerical data were analyzed by using descriptive statistical analysis and the qualitative data by using content analysis. In the diaries, nurses described family grief and mourning as well as content of the supportive telephone call after the death of a family member. Multidimensionality described the family member's grief. The funeral was an important part of the culturally dictated mourning. Support for the family during the patient's last days at the hospital was meaningful with regard to the grief and the onset of coping. Closeness of the nurse-family relationship varied from a close relationship to an uncertain one. A supportive atmosphere during the call made it possible for the survivor to ask questions and talk. During the call, nurses were able to evaluate the family's coping. They also got feedback concerning the nursing care delivered. The call served as a finishing analysis of the family nursing process. The implications of these results for supporting the grieving family by a telephone intervention are considered.

Adaptation, Psychological↗

PubMed2000

The impact of supportive telephone call intervention on grief after the death of a family member.

This study describes the impact of a supportive telephone call on grief 4 months after the death of a family member. The study design involved a quasi-experimental intervention group (n = 70) and a control group (n = 155). The intervention was a supportive telephone call after the death of a family member. Grief reactions were measured with the Hogan Grief Reactions Checklist. Results were completed by content analysis of family members' experiences of the intervention. Chi-square and t tests were used to compare the associations with demographic data, and logistic regression analysis was used to compare the responses. The results pointed to differences in despair and personal growth between the groups. The participants experienced the supportive telephone call positively for the most part. Negative experiences were associated with promises to call in which the call never reached the participant. Grieving family members' positive experiences of the call indicate that there is a need for individual support after the death, given by nurses of the wards in which the deceased received care.

Adolescent↗

PubMed2000

Importance of the midwife in the first-time mother's experience of childbirth.

The aim of this study was to examine factors related to how the first-time mother experiences childbirth. The data were collected by questionnaire in a sample of 326 first-time mothers in Tampere University hospital in Finland. The multivariate method used was a stepwise regression analysis. The most important predictors for the nature of the childbirth experience were the characteristics of the midwife, such as empathy, friendliness, tenderness, calmness and lack of hurry; the attitude of the child's father toward the pregnancy; the duration of delivery; and the mother's age and marital status. The more positive the experience the mother had of the attending midwife caring for her, the more positively she experienced childbirth. The positive attitude of the child's father toward the pregnancy and a short duration of delivery were also predictors for a positive childbirth experience. Young and unmarried mothers also had more positive experiences of childbirth. On the basis of the research results, we can state that positive childbirth experiences of first-time mothers are enhanced by the positive characteristics and professional skills of the attending midwife, positive attitude of the spouse/child's father toward the pregnancy, and a short duration of delivery.

Attitude to Health↗

PubMed1999

Factors related to successful breast feeding by first-time mothers when the child is 3 months old.

The aim of the reported study was to gain information on those factors which contribute to the success of breast feeding in first-time mothers when the child is 3 months old. The study is part of a wider longitudinal project which follows-up the growth to motherhood of first-time mothers in Finland over a period of 8 months postpartum. Data collection was by questionnaires distributed between March and September 1995. The sample comprised 271 first-time mothers. The mothers completed the questionnaires when their infants were 3 months old. The analysis applied was a polychotomic logistic regression analysis. When the child was 3 months old those mothers who perceived themselves competent as mothers and who felt that society appreciated motherhood appropriately coped better with breast feeding. Also, those mothers who had worked at home prior to the birth of the child and who felt that breast feeding was important in motherhood coped better with breast feeding. Those mothers whose state of mind was more balanced after the birth coped better with breast feeding. The more affirmation the mother received from members of her social network the better she coped with breast feeding. The research findings suggest that factors influencing successful breast feeding in first-time mothers at 3 months postpartum are the mother's own resources and attitude to breast feeding, support from the social network and the current appreciation of breast feeding in society.

Adaptation, Psychological↗

PubMed1999

Grief and social support after the death of a spouse.

The death of a spouse is one of the most stressful events in a person's life. Social support has been shown to be widely beneficial in moderating the effects of both chronic and acute stress. The answers of Finnish widows and widowers (n=318) were analysed in order to investigate the sources of social support, what is the nature of support received, and whether social support is connected with coping with grief. The Hogan Grief Reactions Checklist was used to describe the grief as well as answers to open-ended questions about what helped the widowed persons cope with their grief. Kahn's theory of social support was used as a framework in the content analysis of the open-ended answers. The results showed that Finnish widows and widowers receive social support most often from their own family and friends. They perceived the received support most helpful, but also the support that the grieving person can give to other family members is seen as important. Results suggest that those who had had social support were able to grieve by allowing themselves to disorganize and experience panic behaviour.

Adaptation, Psychological↗

PubMed1999

Social support provided by public health nurses and the coping of first-time mothers with child care.

The purpose of this study was to look at the factors related to the first-time mother's coping with child care when her child is 3 months old. This study is part of an extensive longitudinal project in which the development of mothering skills of first-time mothers was followed up for 8 months postpartum. The data were collected with a questionnaire between March and September 1995, in a sample of 271 first-time mothers. The mothers filled in the questionnaire when their babies were 3 months old. Coping with child care was assessed using Spearman correlation coefficient and stepwise regression analysis. Positive correlation was found between the mother's competence, attachment to the child, health, depression, relationship with the spouse, sense of isolation and role restriction, and the mother's coping with child care. The strongest correlation existed between the mother's competence and her coping. Positive correlation was also found between the child's mood, demandingness and acceptability, and the mother's coping with child care, with the child's demandingness having the strongest correlation. Positive correlation was again found between social support--the average functional support the mother received from her own social network, plus affect, affirmation, and aid from the public health nurses--and the mother's coping with child care. The predictors included in the multivariate method were the mother's competence, health, depression, and attachment to her child; the child's mood and ease or difficulty of care; and the affect and affirmation from the public health nurse. The results show that the first-time mother's coping with child care, when the child is 3 months old, is affected by the characteristics of both mother and child, as well as by the social support received from their social network and from the public health nurses at the child welfare clinic.

Adaptation, Psychological↗

PubMed1998

What contributes to breastfeeding success after childbirth in a maternity ward in Finland?

BACKGROUND: The study objective was to gain information about factors that contribute to the successful establishment of breastfeeding in first-time mothers while they are still in the maternity hospital. The study was part of a wider longitudinal project that examined the development of first-time mothers into motherhood during eight months after the birth. METHODS: Data were collected by a questionnaire distributed between January and May 1995. The sample comprised 326 first-time mothers, who completed the questionnaires on about the fifth day after childbirth. A polychotomic logistic regression analysis was applied. RESULTS: Mothers who had a positive experience of breastfeeding in the maternity ward and who began lactating 2 to 3 days postpartum coped better with breastfeeding than those whose experience was less positive and who lactated later. Moreover, the greater the emotional (affect) and concrete (aid) support received by the mother from members of her support network, the better she coped with breastfeeding. By contrast, those mothers who were upset while in the maternity ward coped less well with breastfeeding. CONCLUSIONS: Establishing successful breastfeeding in first-time mothers requires the professional guidance and support of the maternity staff and paying attention to the person closest to the new mother, who in this study was the spouse or father of the child.

Adolescent↗

PubMed1996

Social support provided by nurses to recent mothers on a maternity ward.

The purpose of this study was to look at the social support provided by nurses on a maternity ward to recent mothers and to describe the mothers' experiences of their stay on the ward after childbirth. The data were collected with a questionnaire in a sample of 200 mothers giving birth at Tampere University Hospital. The questionnaire was specially designed for this study by the researcher on the basis of Kahn's (1979) theory of social support. Of the three categories of support identified in that theory, the one that mothers reported receiving most of during their stay on the maternity ward was concrete aid; and the type of support they received least was affect or emotional support. The vast majority or 95% of the mothers described their stay on the ward as a positive experience. Both first-time and other mothers had hoped to receive more guidance in child care and breastfeeding.

Breast Feeding↗

PubMed1996

Social support and its impact on mothers' experiences of childbirth.

The purpose of this study was to look at the networks of social support available for mothers during pregnancy and childbirth. Another concern was to describe mothers' experiences of labour and the connections of social support with those experiences. The data were collected through the use of two questionnaires submitted to 200 mothers recruited from Tampere University Hospital. Both instruments--Norbeck's Social Support Questionnaire and a scale designed by the author--were based on Kahn's (1979) theory of social support. The mothers reported an average of seven support persons each. Most typically, these were the mother's spouse or partner and close friends and relatives. The network of social support is a major source of emotional support, aid and affirmation for the mothers. During labour the main source of emotional support for mothers was the midwife. For the vast majority (85%) of the mothers, childbirth was a positive experience. A significant association was found between the emotional support provided by midwives and mothers' positive experiences of childbirth.

Adolescent↗