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Biomedical subjects

M T Verde

Publications and source records attributed to M T Verde.

10 recordsLinked to original sources
PubMed2006

Influence of colostrum treated by heat on immunity function in goat kids.

The aim was to evaluate the influence of goat colostrum treated by heat on immune function in kids. Thirteen newborn kids were fed untreated colostrum (group A) and 13 kids were fed colostrum treated by heat (56 degrees C, 30 min) (group B). Blood samples were obtained at eight time points between the ages of 0 h to 28 days. Serum protein fractions, IgG levels and phagocytic activity of neutrophils were determined. A delayed type hypersensitivity (DTH) test was used, clinical status and body weight was recorded. There were no clinical signs of disease and no differences (P>0.05) on body weight between groups were noted. Kids from group B had less total protein levels and gamma-globulins than kids from group A (P<0.05). A decrease in serum IgG levels (P<0.05) was observed in kids from group B during all experiment. DTH response in kids from group B was lower (P<0.01) than group A, suggesting alteration on cellular immune system. No effects on phagocytic activity of the neutrophils were observed when both groups were compared (P>0.05). These results showed that colostrum treated by heat impaired some immunological parameters in kids, but these changes did not affect on clinical status or performance.

Animals↗
PubMed2005

High prevalence of ulcerative posthitis in Rasa Aragonesa rams associated with a legume-rich diet.

This study describes an outbreak of ulcerative posthitis that eventually affected 76 of 80 of rams in a flock of Rasa Aragonesa sheep on a legume-rich diet. Lesions were confined to the prepuce and varied from mild hyperaemia to ulcerations. Corynebacterium renale was isolated from the lesions. Treatment of an initial group of 17 was based on a change of diet and topical treatment with Veterin Banedif with prednisolone once a day for 15 days and was successful. Lesions in this group were completely resolved after 15 days. The relationship between a legume-rich diet, ruminal alkalosis, elevated urine pH and posthitis is discussed.

Animal Feed↗
PubMed2000

Effect of aflatoxin on performance, hematology, and clinical immunology in lambs.

Twenty-four female lambs were intoxicated with a diet contaminated with 2 ppm aflatoxin for a period of 37 d. Twelve lambs were maintained as the control group. After this period, the lambs were left for 35 d without aflatoxin in their feed. Performance, hematology and clinical immunology were examined in the intoxicated lambs. A non-significant decrease in body weight was observed in the intoxicated lambs during the intoxication period, whereas a significant decrease (P<0.001) in average daily gain was noted on the last day of intoxication and during the clearance period. No significant differences were observed in erythrocyte count, white blood cell count or differential leukocyte count between the groups. Bacteriostatic activity of the serum was lower in the intoxicated lambs, however, there was no effect on serum opsonic activity. Phagocytosis by the neutrophils was higher during the intoxication period and the levels of IgG were elevated in the intoxicated lambs. In vivo cellular immunity was assessed by intradermal injection of phytohemagglutinin; the response was lower during intoxication period. These results indicate that a lowering in the average daily gain was the most sensitive indicator of aflatoxicosis in lambs, and that the immune response was altered, which could render the animals more susceptible to infectious diseases.

Aflatoxins↗
PubMed1997

Serological serum protein fraction and responses to Brucella melitensis in lambs fed aflatoxins.

Twenty-four lambs were given 2 ppm aflatoxins (AF) in their diet for 37 d and 12 were kept as a control group. After this time, toxic feed was removed for a further 35 days (clearance period). On day 17 all lambs were vaccinated with B melitensis strain Rev-1, and blood samples were taken regularly to determine the levels of antibodies and serum proteins. Aflatoxins decreased titers of Rose Bengal test and optical densities of ELISA, both in the intoxicated and clearance periods. Complement fixation titers were lower in intoxicated lambs, except on the 23rd day of intoxication, but not statistically different (P > 0.05). No effect of AF was noted on total serum proteins, but albumin and alfa-globulin levels were lower for intoxicated lambs than for the control group. Beta-globulin concentration did not change, and increases in gamma-globulins levels in dosed lambs were observed throughout the experiment. These results suggest that AF causes a failure in the acquired immunity system of lambs by decreasing antibody production and altering serum profile proteins.

Aflatoxins↗
PubMed1995

Changes in the prothrombin time, haematology and serum proteins during experimental aflatoxicosis in hens and broiler chickens.

Two groups of 32 laying hens and two groups of 32, 23-day-old broiler chickens were given 2.5 and 5 mg kg-1 of aflatoxin in their diet for four, eight, 16 and 32 days; 16 hens and 32 broiler chicks served as control groups. In the laying hens, aflatoxicosis was characterised mainly by an increase in prothrombin time, and decreases in total plasma proteins and albumin levels; there were increases in red blood cell (RBC) counts and haematocrit which were greater in the group fed 5 mg kg-1. There was a decrease in beta-globulins and an increase in gamma-globulins. In the broiler chickens, there was only a slight increase in prothrombin time and a decrease in RBC counts. This data proves the good sensitivity and utility of prothrombin time values as an indicator of aflatoxicosis in birds.

Aflatoxins↗
PubMed1995

Changes in the coagulation profile of lambs intoxicated with aflatoxin in their feed.

Twenty-three male lambs were intoxicated with 2.5 ppm aflatoxins in their feed for a period of 3 weeks. Thirteen lambs were maintained as a control group (0 ppm aflatoxins in their feed). The coagulation profiles were determined from blood samples that were obtained at 0, 7, 14 and 21 d during the intoxication period and at 1, 2, 4 and 8 d of an 8-d clearance period. Aflatoxicosis in the animals was characterized by an increase in prothrombin time (P < 0.01) from d 14 of the intoxication period until the end of the experiment. An increase (P < 0.05) in fibrinogen concentration was detected beginning on d 21, instead of the expected decrease. This was probably due to the inflammation found in the lungs of the intoxicated animals. No difference in activated partial thromboplastin time was found between intoxicated and control animals. These results suggest that there was a significant change in some coagulation factors of the extrinsic pathway in the intoxicated lambs and that prothrombin time determination could be used as an indicator of aflatoxicosis in lambs.

Aflatoxins↗
PubMedDate not supplied

Efficacy of phosphomycin in the control of Escherichia coli infection of broiler chickens.

Seventy-five 25-day-old broilers were divided into three groups: group I unmedicated and challenged with E. coli O78:K80; group F infected and treated with 150 ppm of phosphomycin in their drinking water, and group C acted as a control. Their weights, feed intake, clinical signs, macroscopic lesions, E. coli reisolation, and serum biochemistry were compared. Group F showed fewer symptoms and gross lesions than those from group I while the average daily gain, bodyweight, and feed intake were similar to the control group. E. coli was reisolated in 32 per cent of the livers and spleens from group I, compared with 4 per cent of liver and 8 per cent of spleens from group F. There was an increase in the levels of total protein and globulins in group I but not in group F. These results provide evidence of the therapeutic efficacy of phosphomycin in the control of an experimental E. coli infection in broiler chickens.

Animals↗