PubMed Health⌕ Search

Biomedical subjects

M Takaya

Publications and source records attributed to M Takaya.

At least 73 records · Page 4Linked to original sources
PubMed1986

[Effects of pentobarbital anesthetic induction on sensory evoked potentials in the cat].

During the last decade, noninvasive electrophysiological tests with sensory evoked potentials have rapidly blossomed into useful clinical tools. They have been studied in large groups of normal subjects and in patients with a wide variety of neurologic diseases. Almost all experimental animal studies on sensory evoked potential, however, have not been undertaken under sufficient consideration on the effects of drugs used for induction into anesthesia on evoked potentials. In order to use evoked potentials as a tool for assessing neurological function in animals, it is necessary to known the effect of anesthetic agents on evoked potentials. Pentobarbital, Somnopentyl, is one of the drugs most commonly used for induction into anesthesia is animal experiments. Authors have attempted to study the long term effects of pentobarbital on evoked potentials. Intramuscular administration of anesthetic dose of pentobarbital was followed by prolongation of 11 and III waves of SEP and I-II interpeak latency of BAEP. Latency of N1 of SEP was also prolonged. On electroencephalogram, amplitude have increased and frequency have got slower after pentobarbital administration. These electrophysiological changes persisted over 5 hours after pentobarbital administration. These results suggest that in some studies on animals the residual effect of agents used for induction into anesthesia must be taken account for analysis of data obtained.

Anesthesia↗
PubMed1985

Circulating natural killer cells in Sjögren's syndrome.

Reduced natural killer (NK) cell activity of peripheral blood lymphocytes (PBL) has been reported in a number of diseases including Sjögren's syndrome (SS). In this study, we used 2 monoclonal antibodies directed toward NK cells (anti-Leu-7 and anti-Leu-11) for determining NK cell activity in 29 patients with SS (9 with primary SS and 20 with secondary SS). The NK activity of PBL was simultaneously determined by the 51Cr release method using K562 as target cells. Contrary to previous reports, we did not find reduced NK activity of PBL in our patients compared with sex- and age-matched healthy controls. Although the percentage of Leu-7+ cells was significantly higher in the patients than in the controls (P less than 0.05), the absolute number of circulating Leu-7+ cells was not different between the groups. The percentage of Leu-11+ cells, however, was not significantly different between the patients and the controls, but the number of circulating Leu-11+ cells was significantly fewer in the patients than in the controls (P less than 0.05). Between the primary and secondary SS groups, no significant differences were found in NK cell activity or in the percentage of Leu-7+ or Leu-11+ cells. Furthermore, we found a significant correlation of NK activity with the percentage of Leu-11+ cells (P less than 0.05) in the controls as well as the SS patients, although a significant correlation was not identified between NK activity and the percentage of Leu-7+ cells.(ABSTRACT TRUNCATED AT 250 WORDS)

Female↗
PubMed1985

[Effect of intravenous administration of diltiazem on cerebral blood flow and brain function].

The effect of intravenous administration of diltiazem on cerebral circulation and brain function were studied in animals. Diltiazem, at a dose of 150 micrograms/kg BW, was administered intravenously over a period of 5 minutes beginning 30 minutes after ligation of the right brachiocephalic artery in 9 cats in order to investigate its effects in the acute stage of cerebral ischemia. In 5 animals, mean arterial blood pressure was recorded and blood flow in the basilar artery was measured transdurally by use of an ultrasonic Doppler flow-meter. Mean arterial blood pressure began to elevate immediately after ligation of the right brachiocephalic artery, but the degree of elevation was minimal. It began to fall during diltiazem injection and returned to the preadministration value 30 minutes after injection. Basilar artery flow decreased slightly during injection of diltiazem, but began to increase after completion of injection. The increase relative to the preadministration value was 62 +/- 31% 10 minutes after injection, 88 +/- 38% 20 minutes after, and 24 +/- 53% 30 minutes after. The short latency somatosensory evoked potentials (short latency SEP) recorded in the other 2 animals showed no noticeable change either in amplitude or latency during the same procedures. In 1 of 2 animals in which brainstem auditory evoked potentials (BAEP) were monitored, the latency of peak IV increased after ligation of the right brachiocephalic artery, and decreased 20 minutes after injection of diltiazem.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1985

[Effects of phenobarbital on EEG and sensory evoked potentials in normal cat brain].

In adult cats, authors studied changes in parameters obtained by the recordings of sensory evoked potentials following cumulative intravenous administration of phenobarbital. P1 and N1 of cortical SEP showed gradual tendencies to decrease in amplitude and to increase in latency with increasing barbiturate dose. These changes preceded to the appearance of burst-suppression activity on electroencephalogram. At this point I-II and III-IV interpeak latencies of BAEP showed statistically significant increase. BAEP and all early components of SEP (I.II.III) persisted in the extremely high serum concentration of phenobarbital. Furthermore, II-III interpeak latency of BAEP showed no statistically significant increase. These experimental results suggest that sensory evoked potentials will provide useful information in the assessment of the brainstem function in patients under deep coma.

Animals↗
PubMed1984

Solubilization of intraglomerular deposits of IgG immune complexes by human sera or gamma-globulin in patients with lupus nephritis.

A study of the solubilization of glomerular deposition of IgG immune complexes by sera from patients with lupus nephritis is described. Renal biopsy specimens were obtained from 11 patients with lupus nephritis, five patients with IgA nephropathy and one patient with minimal change nephrotic syndrome. These renal specimens were incubated with fresh, stored or heated sera from the same patients or healthy adults and human gamma-globulins at 37 degrees C for 1 h in plastic test tubes. The sections were stained with FITC conjugated heavy chain specific anti-human IgG or C3 antisera and then examined with a fluorescent microscope. The sections were also stained with FITC conjugated human gamma-globulins and rhodamine conjugated anti-human IgG, IgM or IgA antisera and then examined by double exposure under a fluorescent microscope. It was demonstrated that fresh human sera or gamma-globulins significantly solubilize glomerular immune deposits in patients with lupus nephritis in vitro. It was indicated that the solubilization of IgG glomerular deposits from patients with lupus nephritis does not depend on complement. It is postulated that solubilization of immune deposits in glomeruli requires the excess amounts of antigenic substances in patients with lupus nephritis.

Antigen-Antibody Complex↗
PubMed1982

Experimental induction of sialadenitis in rats by heterologous anti-salivary gland antibodies.

The purpose of the present study was to investigate the possibility of experimental induction of sialadenitis in rats by heterologous anti-salivary gland antibodies. Two fractions (Fraction I and II) were separated from rat submaxillary gland by Sephadex-G 200 gel-filtration, and then rabbits were immunized with each fraction. Although cross-reactivity of anti-FrI antiserum obtained from the rabbits against rat liver and rat serum was shown, specific reactivity of both anti-FrI and anti-FrII antisera against rat submaxillary gland was demonstrated by immunodiffusion or the indirect immunofluorescence method. Activity of antibody-dependent cell-mediated cytotoxicity against fresh rat submaxillary gland was also demonstrated using the anti-FrII antiserum in vitro. Sialadenitis with periductal cell infiltrations was induced in the submaxillary glands of many rats injected intraperitoneally with each heterologous antiserum. Similar lesions, however, were also induced in the parotid glands, livers, or kidneys of these rats. By a direct immunofluorescence method, granular deposits of rabbit gammaglobulin were detected in cytoplasma of the infiltrating cells and the small vessels around the lesions of the submaxillary glands. It was suggested, therefore, that the lesions are induced by circulating immune complexes probably formed by non-organspecific antibodies contaminating each antiserum. Salivary gland lesions induced by a similar mechanism were also suggested in patients with Sjögren's syndrome, although the pathological significance in vivo of the anti-salivary gland antibody as an organspecific antibody could not be confirmed by the present study.

Animals↗
PubMed1981

Clinical evaluation of 117 patients with Sjögren's syndrome.

The clinical entity of Sjögren's syndrome was limited to exocrine gland involvements in the present study, and 117 patients fulfilling this criterium were evaluated clinically to clarify the features of this syndrome. The following conclusions were obtained. (1) Frequent overlapping of this syndrome with other autoimmune diseases was observed, (2) This syndrome was demonstrated in young patients and middle-aged as well as postmenopausal patients. (3) Some of systemic manifestations demonstrated in patients with associated diseases were also observed in many patients without definite associated diseases. (4) Various hematological and serological abnormalities were demonstrated even in patients without associated diseases, and therefore, the participation of either a chronic inflammatory or immunologic process was suggested in this syndrome. (5) In patients with associated diseases, influence of the associated diseases was prominent in accordance with the patients' age, systemic manifestations and serological abnormalities. (6) The subclinical nature of sicca symptoms in young patients, as well as the delayed advancement of glandular involvement in this syndrome, were suggested. (7) Different entities were suggested in some patients with only lachrymal involvement.

Adult↗
PubMed1981

Characterization of sicca symptoms in patients with Sjögren's syndrome, and reports of six cases lacking subjective sicca features ("subclinical Sjögren's syndrome").

We evaluated seventeen ocular and oral symptoms in patients with Sjögren's syndrome (SjS) to characterize the subjective sicca symptoms of this syndrome. The following eleven symptoms were characteristic in patients with SjS compared with patients with rheumatoid arthritis (RA) or healthy adults.: (1) decreased lachrymation, (2) ocular foreign body sensation, (3) ocular filmy sensation, (4) increased mucous discharge from the eyes, (5) thirsty sensation, (6) decreased salivation, (7) increased dental caries, (8) abnormal sense of taste, (9) difficulty in chewing, (10) dryness of the oral cavity, and (11) increased fluid-intake with meals. Some of the symptoms were detectable in RA patients and healthy adults although the mean number of the positive symptoms was significantly higher in patients with SjS. The demonstration of three or more symptoms, therefore, will be sufficient in the consideration of the definite sicca features for this syndrome. The present studies also confirmed the chronological influence on the development of subjective sicca features in patients with SjS, and support our concept that patients lacking subjective sicca features are those in the early stages of this syndrome. Finally, six cases of SjS lacking subjective sicca features were described.

Adult↗