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Biomedical subjects

M Tariq

Publications and source records attributed to M Tariq.

At least 19 recordsLinked to original sources

Hypobaric spinal anaesthesia with bupivacaine (0.1%) gives selective sensory block for ano-rectal surgery.

Twenty adult male patients undergoing anorectal surgery in the jackknife position under spinal anaesthesia were studied for the anaesthetic properties of 5 ml hypobaric 0.1% bupivacaine. The patients were positioned in the prone, jack-knife position with a pillow under the hips and with an operating table break angulation of 30 degrees with head down tilt of 20 degrees. In this position a 25-gauge Quincke spinal needle was inserted intrathecally through L3-4 and 5 ml solution, prepared by mixing 1 ml bupivacaine 0.5% with 4 ml of distilled water with a specific gravity of 1.001 at 20 degrees C, was given over 15-20 sec. Onset time, progression and upper level of sensory blockade evaluated by pin prick, and the extent of motor block (1 = full motor movement at ankle and knee joint, 2 = restricted motor movements, 3 = full motor block, no movements) were measured at one minute intervals for the first five minutes, then every five minutes for 30 min. The number of dermatomes blocked was also noted. The median level of cephalad sensory blockage was of L1, with a range from T10-L3. On average, nine dermatomes were blocked (range 7-12). Motor blockade was not observed in any patient. Changes in heart rate and blood pressure were minimal. The average duration of postoperative analgesia was 339.5 +/- 182.9 min. Post-spinal headache was not observed in any patients. In conclusion, 5 ml intrathecal hypobaric bupivacaine, 0.1%, provided excellent perioperative analgesia without motor blockade and haemodynamic stability in patients undergoing anorectal surgery in jackknife position.

Adolescent

Dipyridamole attenuates the development of iminodipropionitrile-induced dyskinetic abnormalities in rats.

The present investigation was undertaken to study the effect of dipyridamole on experimental dyskinesia in rats. The movement disorders were produced by intraperitoneal administration of iminodipropionitrile (IDPN) in the dose of 100 mg/kg per day for 12 days. Dipyridamole was administered orally, daily 30 min before IDPN in the doses of 0.5 g/kg, 1 g/kg, and 1.5 g/kg bodyweight in three different groups of rats. Twenty-four hours after the last dose of IDPN, animals were observed for neurobehavioral changes including vertical and horizontal head weaving, circling, backwalking, grip strength, and righting reflex. Immediately after behavioral studies brain specimens were collected for analysis of vitamin E, conjugated dienes, and lipid hydroperoxides as indices of oxygen-derived free radical (OFR) production. Our results showed that concurrent use of dipyridamole significantly protected rats against IDPN-induced neurobehavioral changes in a dose-dependent manner. Treatment of rats with dipyridamole inhibited IDPN-induced decrease of vitamin E and increase in conjugated dienes and lipid hydroperoxides in brain. These findings suggest the involvement of OFR in dipyridamole induced protection against the development of IDPN dyskinesia. Further studies are warranted to determine the role of dipyridamole as a prophylactic agent against the drug induced dyskinetic abnormalities.

Animals

Neuroprotective effect of selenium on iminodipropionitrile-induced toxicity.

The present investigation was undertaken to study the effect of selenium on experimental dyskinesia in rats. The movement disorders were produced in rats by intraperitoneal administration of iminodipropionitrile (IDPN) in the dose of 100 mg/kg per day for 12 days. Selenious acid was administered daily 30 minutes before IDPN in the doses of 5 mumol/kg, 10 mumol/kg and 20 mumol/kg bodyweight in three different groups of rats. Animals were observed daily for any neurobehavioral changes including circling, backwalking, head weaving and twitching. Immediately after behavioral studies, blood and brain specimens were collected for analysis of thiobarbituric acid reactive substances (TBARS) to measure the extent of free radical production. Our results showed that concurrent use of selenium significantly inhibited IDPN-induced neurobehavioral changes in a dose-dependent manner. Treatment of rats with selenium also reduced the TBARS production in blood and different regions of brain. These findings suggest that selenium attenuates the IDPN-induced neurotoxicity by inhibiting lipid peroxidation.

Animals

Effect of (+/-)-verapamil and hydralazine on stress- and chemically-induced gastric ulcers in rats.

The influence of (+/-)-verapamil and hydralazine on stress- and various chemically-induced gastric ulcers in rats together with their influence on various biochemical parameters which affect the development of the induced ulcers was examined. Pretreatment of rats with (+/-)-verapamil (4-16 mg kg-1 orally) significantly decreased cold-stress-induced gastric ulcers and enhanced ethanol-induced ulcers. It did not affect indomethacin- (30 mg kg-1 orally) or reserpine- (5 mg kg-1 i.p.) induced ulcers. Pretreatment of the animals with hydralazine (1-10 mg kg-1 orally) significantly enhanced ethanol-, reserpine- and cold-stress-induced ulcers. It did not affect indomethacin-induced ulcers. Pretreatment of the animals with verapamil increased gastric mucus secretion, inhibited gastric acid secretion, decreased glutathione content and enhanced gastric lipid peroxidation whereas pretreatment of the animals with hydralazine significantly decreased gastric mucus secretion. Hydralazine did not affect gastric acid secretion, glutathione or gastric lipid peroxidation. The results of this study suggest that verapamil-induced protection against stress-induced ulcer may be due to its ability to suppress gastric acid secretion and to increase gastric mucus secretion. Its enhancement of ethanol-induced ulcers may be due to its ability to increase lipid peroxidation. The hydralazine-induced enhancement of the experimentally-induced ulcers may be due to its ability to suppress gastric mucus secretion.

Animals

Effect of selenium and vitamin E on iminodipropionitrile induced dyskinesia in rats.

The present study was undertaken to determine the effect of combination of selenium and vitamin E on experimentally induced dyskinesia in rats. The dyskinetic syndrome was produced in 4 groups of 6 male rats each weighing 250-300g by intraperitoneal (ip) administration of iminodipropionitrile (IDPN) in doses of 100 mg/kg body weight daily for 12 days. A group of 6 rats (group 1) served as control and received normal saline only. The rats in group 2 (IDPN only) received normal saline (ip) 30 minutes before the administration of IDPN. The animals in groups 3, 4 and 5 received selenous acid (5 mumol/kg), vitamin E (500 mg/kg p.o.) and a combination of selenous acid and vitamin E respectively, daily, 30 minutes before IDPN for 12 days. Twenty four hours after the last dose of IDPN, the dyskinetic behavior including vertical head movements (retrocollis), horizontal head movements (laterocollis), circling and backwalking of each rat was studied for a period of 10 minutes. Immediately after behavioral studies, the animals were sacrificed and brains were dissected out for the analysis of conjugated dienes, lipid hydroperoxides and vitamin E. The results of this study showed that treatment of rats with IDPN only for 12 days produced dyskinetic syndrome in all the rats characterized by vertical and horizontal head movements, circling and backwalking. Concomitant treatment of rats with vitamin E and selenium individually reduced IDPN induced dyskinesia, and the symptoms were almost completely absent when the combination of these two agents was used.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals

Cod liver oil inhibits indomethacin induced gastropathy without affecting its bioavailability and pharmacological activity.

The upper gastrointestinal toxicity is one of the most common side effects associated with the use of nonsteroidal anti-inflammatory drugs (NSAIDs). Many attempts to prepare potent NSAIDs free from gastrotoxicity have failed. Hence, development of formulations to mask the gastropathy of NSAIDs are warranted. The present study was undertaken to investigate the effect of concomitant use of cod liver oil (CLO) on pharmacological activity and gastropathy of indomethacin in rats. The animals were treated with CLO (5 and 10 ml/kg body weight) along with indomethacin (30 mg/kg, body weight). Blood samples were collected for analysis of indomethacin at 0.25, 0.5, 1.0, 1.5, 2.0, 4.0, 6.0 and 24 hours. The anti-inflammatory activity of indomethacin alone and in combination with CLO was studied using carrageenan-induced paw oedema. Our studies related to the effect of these drugs on gastrointestinal tract showed that concurrent use of CLO protects gastric mucosa against indomethacin induced depletion of gastric wall mucus, non protein sulfhydryl (NP-SH) levels and gastric lesions. The result of this study also showed that the concurrent use of the CLO does not affect the bioavailability and anti-inflammatory activity of indomethacin while it inhibits the ulcerogenic effect of indomethacin in a dose dependent manner. These findings suggest that NSAIDs formulations containing CLO may reduce gastrotoxicity without affecting their therapeutic efficacy.

Administration, Oral

The toxicity of Catha edulis (khat) in mice.

A large number of people in East Africa and Southern Arabia chew khat leaves because of its pleasurable and stimulating effects. Due to its habit forming property, the khat has been classified as a "Substance of Abuse" by the World Health Organization. In view of the large number of medical problems reported in khat chewers, the present study was undertaken to investigate the chronic toxicity of khat in mice. Three groups of mice were treated with aqueous solution of khat extract in the dose of 50, 100, and 200 mg/kg. body weight daily by oral intubation route for 6 weeks. The results indicated a dose-dependent decrease in body weight, an increase in the incidence of mortality and induction of site specific body and eye lesions. The histopathological examination of the lesions revealed reactive hyperplasia and necrosis in the lymphoid tissues. The necrotic areas in the subcutaneous tissues showed the presence of numerous polymorphs.

Animals

Pharmacological studies on aerial parts of Calotropis procera.

The decoction of the aerial part of Calotropis procera is commonly used in Saudi Arabian traditional medicine for the treatment of variety of diseases including fever, joint pain, muscular spasm and constipation. The present investigation was undertaken to confirm its claimed activity in traditional medicine. The ethanol extract of the plant was tested on laboratory animals for its antipyretic, analgesic, anti-inflammatory, antibacterial, purgative and muscle relaxant activities. The results of this study showed a significant antipyretic, analgesic and neuromuscular blocking activity. On smooth muscle of guinea pig ileum, the extract produced contractions which was blocked by atropine supporting its use in constipation. The extract failed to produce significant anti-inflammatory and antibacterial activities. Our phytochemical studies on the aerial parts of C. procera showed the presence of alkaloids, cardiac glycosides, tannins, flavonoids, sterols and/or triterpenes. However, the chemical constituents responsible for the pharmacological activities remains to be investigated. The safety evaluation studies revealed that the use of extract in single high doses (up to 3 g/kg) does not produce any visible toxic symptoms or mortality. However, prolong treatment (90 days) causes significantly higher mortality as compared to control group.

Animals

An evaluation of the male reproductive toxicity of cathinone.

(-)-Cathinone is the major psychoactive component of khat plant (Catha edulis Forssk.). Khat has been shown to produce reproductive toxicity in human beings and experimental animals. However, the chemical constituents of khat leaves responsible for sexual dysfunction are not known. In the present study cathinone enantiomers have been investigated for their reproductive toxicity in rats. Cathinone produced a dose-dependent decrease in food consumption and suppressed the gain in body weight. There was a significant decrease in sperm count and motility and increase in the number of abnormal sperms in cathinone treated animals. Histopathological examination of testes revealed degeneration of interstitial tissue, cellular infiltration and atrophy of Sertoli and Leydig's cells in cathinone treated animals. Cathinone also produced a significant decrease in plasma testosterone levels of the rats. Although both enantiomers of cathinone produced deleterious effects on male reproductive system, (-)-cathinone was found to be more toxic. From this study it may be concluded that the cathinone content in khat may be partially or totally responsible for the reproductive toxicity in khat chewers.

Alkaloids

The induction of dominant lethal mutations upon chronic administration of khat (Catha edulis) in albino mice.

The mutagenicity of a methanolic extract of khat has been evaluated on male germ cells using the dominant lethal test in albino mice. An aqueous solution of khat extract was administered orally in doses of 50, 100 and 200 mg/kg body wt., respectively, to 3 different groups of male mice for a period of 6 weeks. At the end of treatment each male mouse was allowed to mate with 2 different groups of 3 females each, on 2 consecutive weeks. These females were necropsied on the 13th day of their presumptive mating, and the number of implants in each female and the ratio of live and dead embryos were determined. The results of this study showed that the treatment of male mice over a period of 6 weeks produced a dose-dependent reduction in the rate of fertility in the first week after mating, which was irreversible in the second week at the highest dose (200 mg/kg). Khat extract also induced post-implantation loss during the first week following treatment. However, a comparison of the results of the first and second weeks showed a reversible pattern of dominant lethality.

Animals

Preliminary toxicity studies on ethanol extracts of the aerial parts of Artemisia abyssinica and A. Inculta in mice.

Ethanolic extracts of the aerial parts of Artemisia abyssinica and A. inculta were subjected to acute toxicity observations in mice for 24 h and chronic toxicity evaluation for 3 months. External morphological changes, visceral toxicity, haematological changes, spermatogenic dysfunction and effect on body weight and vital organ weight were recorded. In both the chronically treated groups, no significant acute mortality was observed up to 3 g/kg p.o. There was no weight gain in A. abyssinica chronically-treated mice while the weight gain of A. inculta-treated animals matched that of the control group. Significant sperm damage was observed in A. abyssinica-treated mice while A. inculta failed to produce any significant spermatotoxic effect.

Alopecia

Studies on Ruta chalepensis, an ancient medicinal herb still used in traditional medicine.

An ethanolic extract of the aerial parts of Ruta chalepensis was studied for its anti-inflammatory, antipyretic, analgesic and CNS depressant activities. The extract produced a significant inhibition of carrageenan-induced paw oedema and cotton pellet granuloma in rats. The studies on spontaneous motor activity in mice and conditioned avoidance responding (CAR) in rats showed a dose-dependent depression of the central nervous system in treated animals. Reduction of yeast-induced hyperthermia in mice confirmed its reputed antipyretic activity. The extract did not produce any significant changes in prothrombin time and fibrinogen level. It also failed to produce any analgesic activity in the hot plate reaction-time test in mice. Phytochemical screening of the aerial parts of the plant showed the presence of alkaloids, flavonoids, coumarins, tannins, volatile oil, sterols and/or triterpenes.

Animals

Evaluation of turmeric (Curcuma longa) for gastric and duodenal antiulcer activity in rats.

An ethanol extract of turmeric was studied in rats for its ability to inhibit gastric secretion and to protect gastroduodenal mucosa against the injuries caused by pyloric ligation, hypothermic-restraint stress, indomethacin, reserpine and cysteamine administration and cystodestructive agents including 80% ethanol, 0.6 M HCl, 0.2 M NaOH and 25% NaCl. An oral dose of 500 mg/kg of the extract produced significant anti-ulcerogenic activity in rats subjected to hypothermic-restraint stress, pyloruic ligation and indomethacin and reserpine administration. The extract had a highly significant protective effect against cystodestructive agents. The reduction in the intensity of ulceration of cysteamine-induced duodenal ulcers was not found to be statistically significant. Turmeric extract not only increased the gastric wall mucus significantly but also restored the non-protein sulfhydryl (NP-SH) content in the glandular stomachs of the rats.

Animals

Effect of khatamines and their enantiomers on plasma triiodothyronine and thyroxine levels in normal Wistar rats.

The effect of cathinone and N-formylnorephedrine, two psychoactive amines of khat (Catha edulis Forsk.) and their enantiomers have been studied on plasma levels of triiodothyronine (T3) and thyroxine (T4) in male Wistar rats. The rats were injected with 5, 10 and 30 mg/kg, body weight of four khatamines and the blood samples were collected 2 h after their administration. In the separate set of experiments the effect of these khatamines at 1, 2 and 4 h after their administration was also examined. All the khatamines failed to produce a significant dose dependent increase in T3 and T4 levels in the dose of 5 mg/kg. However, all of these compounds produced a significant dose dependent increase in T3 and T4 levels at higher doses but only T4 levels were increased following the dose of 10 mg/kg. Our studies on the effect of khatamines in T3 and T4 levels at various times showed a significant increase in T4 levels in all the four groups treated with various khatamines and the peak effect was observed at 2 h in case of (-)- and (+)-cathinone and 4 h in case of (-) and (+)N-formylnorephedrine. This study suggests that the symptoms observed in khat chewers including hyperthermia, anorexia, and metabolic changes may to some extent be attributed to the thyroid stimulating effect of khatamines. However, further studies are needed to establish the mechanism of release of thyroid hormones by these compounds and their involvement in the pharmacological effects.

Alkaloids

Comparison of intravenous and nasal bioavailability of clonidine in rodents.

The bioavailability and cardiac depressant activity of 3H-clonidine was studied following intravenous and nasal administration in rodents. The drug is rapidly absorbed by nasal route, and the peak blood concentration is reached within 10 minutes. The area under the blood concentration-time curve, following intravenous and nasal routes, was found to be 3.55 x 10(5) counts/g/min and 3.75 x 10(5) counts/g/min respectively. The drug was found to eliminate slowly from blood. The t1/2 beta, following intravenous and nasal administration, was found to be 8.8 hr and 8.0 hr respectively. Our electrocardiographic studies, to compare myocardial depression following intravenous and nasal administration of clonidine, revealed that a bolus intravenous clonidine in the dose of 10 micrograms, 30 micrograms, and 100 micrograms/kg body weight produced a significant and transient decrease in heart rate in a dose dependent manner. One rat developed arrhythmia after receiving a higher dose of 100 micrograms/kg body weight of clonidine by intravenous route. However, only a mild decrease in heart rate was observed following nasal administration of clonidine. The examination of the nasal cavity one hour after the single dose of 100 micrograms/kg body weight of clonidine in rats showed no sign of erythema, oedema or lesions. These findings suggest that the nasal route may be a good substitute for I.V. administration of clonidine.

Administration, Intranasal

Gastric antiulcer and cytoprotective effects of dipyridamole in rats.

Dipyridamole has been studied for its ability to inhibit gastric secretion and to protect gastric mucosa against the injuries caused by hypothermic restraint stress, indomethacin and various necrotizing agents including 80% ethanol, 0.6 M HCl, 0.2 M NaOH and 25% NaCl in rats. The results of this study demonstrate that dipyridamole has both prophylactic and curative effects on various experimentally induced gastric ulcers. It produced inhibition of normal and histamine-stimulated gastric secretion in rats. The intensity of gastric lesions induced by indomethacin and hypothermic restraint stress was reduced significantly by dipyridamole. Our findings also showed that dipyridamole protect gastric wall against hypothermic restraint stress-induced mucus depletion. It produced marked cytoprotective effect against all the necrotizing agents used in this study. The cytoprotective effect of dipyridamole against 80% ethanol was reversed significantly by prior treatment with a dose of indomethacin that inhibits prostaglandin biosynthesis. These data indicate that dipyridamole inhibits the formation of gastric lesions by mucosal generation of prostaglandins. The concentration of nonprotein sulfhydryls were decreased significantly in gastric mucosa after administration of 80% ethanol. Treatment with dipyridamole replenish the reduced level of gastric mucosal nonprotein sulfhydryls, thus suggesting the mediation of its protective effect through sulfhydryls. Our findings show that dipyridamole possesses both antisecretory and antiulcer effects. Further studies are required to determine its role in the prophylaxis and or the treatment of gastric ulcer disease.

Animals

Comparative study of cathinone and amphetamine on brown adipose thermogenesis.

The effect of cathinone and amphetamine on brown adipose tissue thermogenesis and its modification with propranolol and timolol has been studied in rats. Both cathinone and amphetamine produced significant dose dependent increases in intracapsular brown adipose tissue (IBAT) and rectal temperatures. Amphetamine was found to be three times more potent as compared to cathinone, on a dose basis. Pretreatment of animals with propranolol and timolol individually inhibited cathinone and amphetamine induced hyperthermia. These findings suggest the involvement of beta adrenergic receptors in cathinone and amphetamine induced thermogenesis.

Adipose Tissue, Brown

Effect of Trigonella foenum-graecum and Ammi majus on calcium oxalate urolithiasis in rats.

The present study was undertaken to investigate the effect of Trigonella foenum-graecum seed and Ammi majus fruit on experimentally-induced kidney stones. Oxalate urolithiasis in male rats was produced by the addition of 3% glycolic acid to their diet. After 4 weeks, highly significant deposition in the kidneys was noticed and changes in water intake and body weight recorded. Daily oral treatment with T. foenum-graecum significantly decreased the quantity of calcium oxalate deposited in the kidneys thus supporting its use in Saudi folk medicine. The effects obtained by A. majus were, however, not significant.

Animals