The interaction between the public and private sectors in hospital hearing aid provision.
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Biomedical subjects
Publications and source records attributed to M Tate.
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The purpose of this study was to provide descriptive information on migrant farm laborers' infant feeding practices in northern Colorado. We used a survey questionnaire to interview 49 families with a 6- to 23-month-old child who was enrolled in the Migrant Head Start Center. Sixteen (33%) of the mothers breast-fed their infants, but only five (14%) of the 35 Mexican Americans breast-fed. Thirty-four (69%) of the mothers used formula and seven (14%) gave their child cow's milk at birth. Introduction of solids occurred at a mean of 6.6 months. Of the respondents, 13 (27%) introduced solids before 4 months of age, whereas 29 (59%) introduced solids before the age of 6 months. Thirty-six (73%) of the migrants put their child to bed with a sweetened beverage, milk, or formula in a bottle. Diarrhea in the children was reported by 39 (80%) of the migrants, with 15 (31%) stating that it occurred at least once per month. One third (n = 16) of the respondents reported that their children had constipation lasting 1 to 14 days, but for most (n = 10) it occurred less than once per month. Results suggest that migrant families need more dietary recommendations about breast-feeding, introduction of liquids/solid foods, proper formula preparation, avoidance of baby bottle tooth decay, and treatment of diarrhea and constipation.
A method was developed to record interactions between mobile non-adherent immunocytes by time-lapse cinematography and then to study the same cells by immuno-electron microscopy, using monoclonal antibodies against surface components. For this purpose a modified stage was designed to fit an inverted microscope. The attachment included a device to cool the culture chamber with N2 gas, a micro-injector for monoclonal antibody and immuno-gold treatment, and two pairs of washing needles to change the medium without disturbance. The technique was first employed to study the formation of aggregates around the antigen-presenting cells in cultures containing cells from hyper-immunized animals. Recently peripheral blood cells from normal subjects and patients with immune deficiency syndromes were stimulated with pokeweed mitogen, cluster formation was recorded, and the cells were processed for immuno-electron microscopy.
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Lymphoblastoid cells transformed by Epstein-Barr virus from peripheral lymphocytes of normal individuals and I-cell disease (ICD) patients were used for the enzymic study of lysosomal hydrolases and N-acetylglucosamine 1-phosphotransferase. ICD lymphoblastoid cells secreted a larger amount of hydrolases into medium than normal cells, although the intracellular hydrolases were not deficient in ICD cells. The stimulating effect of 10 mM ammonium chloride on secretion of hydrolases was found only with normal cells, and not with ICD cells, indicating that the hydrolase molecule bearing mannose 6-phosphate was secreted. The ICD lymphoblastoid cells retained the enzymologic characteristics of both lysosomal hydrolases and N-acetylglucosamine 1-phosphotransferase seen in ICD fibroblasts, which allows us to study the pathophysiology of ICD in cells other than fibroblasts.