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Biomedical subjects

M Tateyama

Publications and source records attributed to M Tateyama.

At least 19 recordsLinked to original sources
PubMed1992

Fluctuations in plasma levels of thrombomodulin in patients with DIC.

Plasma thrombomodulin (TM) has attracted considerable attention as a marker of endothelial cell membrane injury. We examined fluctuations in plasma TM levels in patients receiving therapy for the disseminated intravascular coagulation syndrome (DIC) using an enzyme immunoassay. Sixty healthy controls and 18 patients with DIC were studied. The mean +/- SD of the TM values initially measured immediately after the onset of DIC was 42.00 +/- 20.85 ng/ml, which was markedly increased as compared with the control value of 15.36 +/- 4.85 ng/ml (p < 0.001). Fluctuations in the TM levels over time were studied after dividing the patients according to the presence or absence of improvement in the underlying disease and improvement or lack thereof in the coagulation findings. Group I showed improvement in both categories, Group II showed improvement only in the latter, and Group III showed no improvement in either category. In Group I, the mean +/- SD of initial measured TM levels was 37.02 +/- 10.12 ng/ml and the mean of final values decreased to 58.9% of the initial value. This decrease was significant by paired Student's t-test (p < 0.01). The initial value in Group II was 45.86 +/- 18.86 ng/ml and the final values increased to 117.0% of the initial values, this difference was not significant. The initial value in Group III was 44.48 +/- 21.53 ng/ml and the final values increased to 143.4% of the former. This increase was significant by paired Student's t-test (p < 0.05). The difference in % fluctuations between Group I and Group III was significant by Wilcoxon's test (p < 0.01). These results suggest that the measurement of plasma TM can be useful in the management of DIC.

Adolescent↗
PubMed1992

Cytotoxicity of mebendazole against established cell lines from the human, rat, and mouse liver.

The direct cytotoxicity of mebendazole (MBZ) was investigated by using cell lines derived from human, mouse and rat liver. It was demonstrated that Chang liver cells (derived from human liver) were more sensitive to the cytotoxic effects of MBZ than the other two cell lines. Longer incubation of the cells with MBZ resulted in stronger toxicity, and the cytotoxicity was dependent on the MBZ concentration above a certain threshold value (0.25-0.50 mg/l in a 42-h culture). Inhibition of the proliferation of Chang liver cells by MBZ was detected at a concentration of 0.008 mg/l, a lower concentration than that having a cytotoxic effect. The other two cell lines were less sensitive to the inhibitory effect of MBZ. Proliferation of human mononuclear cells following stimulation by phytohemagglutinin (PHA) was inhibited by MBZ, and this inhibition was more extensive than that of cells stimulated with whole formalin-treated Pseudomonas aeruginosa. It is suggested that dividing cells may be more sensitive to MBZ cytotoxicity. This anti-proliferative effect may be related to its clinically known side effects, such as hepatotoxicity and bone marrow suppression.

Animals↗
PubMed1992

[Detection of Legionella pneumophila using a nested polymerase chain reaction].

We evaluated the usefulness of a Nested PCR method for detecting Legionella pneumophila. This method resulted in L. pneumophila specific detection as far as we evaluated. The first and second step PCR achieved the sensitivity as small as 10 pg and 10 fg of the target DNA, respectively. In the detection from Legionella seeded sputa, the method could detect 0.1 cfu/ml of the bacteria, and it took about 12 hours to detect the target DNA. We demonstrated that the Nested PCR method was superior in sensitivity and rapidity for isolation of the bacteria to the conventional using low pH treatment and selective media for Legionella.

Base Sequence↗
PubMed1992

Misleading serological identification of Legionella anisa as Legionella bozemanii.

Identification of six Legionella species, which we previously identified by serological test as Legionella bozemanii (L. bozemanii), was performed by DNA-DNA hybridization using a commercial DNA-DNA hybridization kit (Kobayashi Pharm. Co., Japan) introduced by Ezaki et al. All strains were identified as Legionella anisa (L. anisa), this being the first identification of L. anisa in Japan. Conventional laboratory tests were performed following the DNA-DNA hybridization. In this study the results of biochemical examination obtained, corresponded closely with those described in previous reports, but the oxidase reaction was very weak and varied according to the age of the culture, indicating the unreliability of this test in our case. All strains examined under long wave ultraviolet (UV) light (366 nm) revealed a blue-white fluorescence, the intensity of which ranged from strong to weak. Serological identifications were performed by both the slide agglutination test (SAT) and indirect immunofluorescent assay (IFA). SAT using commercially available antiserum (Denka Seiken., Japan) supposedly specific for L. bozemanii showed cross-reaction between L. bozemanii and L. anisa. Hyperimmune rabbit antisera prepared in this study for both L. bozemanii and L. anisa, from which cross-reactive antibodies were removed by the absorption of each antigen, reacted only with homologous antigens. IFA using a commercially available antiserum and hyperimmune rabbit antiserum previously described, gave positive reactions with each strain.

Agglutination Tests↗
PubMed1992

[Mechanism of acquired resistance against Legionella--blastogenic responses of murine spleen lymphoid cells following the stimulation with Legionella antigens].

We have tried to characterize the blastogenic responses of murine spleen lymphoid cells from BALB/c mice immunized with Legionella pneumophila serogroup (SG) 1 (Philadelphia 1 strain) and non-treated mice. Lymphoid cells from immunized mice showed stronger blastogenic responses following stimulation with concanavalin A or formalin-treated L. pneumophila SG1 whole cell antigen than those showed by lymphoid cells from non-treated mice. These cells from immunized mice also responded strongly when stimulated in vitro with other SGs of L. pneumophila, while these responded weakly when stimulated with other species of Legionella. Serum antibody titers of immunized mice against each SG of L. pneumophila were examined and the cross reactions were also recognized. However, the relatedness of serum antibody titers and the blastogenic responses against each serogroup of L. pneumophila was small. The epitopes recognized by the cellular immunity might be different in part from those recognized by serum antibodies, and investigations should be made on what the cellular immunity recognizes and how it works.

Animals↗
PubMed1992

Urinary albumin determination by gel-filtration high-performance liquid chromatography.

To determine urinary albumin in a minute amount, a gel-filtration high-performance liquid chromatographic procedure was newly established. When urine of a normal rat was eluted isocratically at 0.6 ml/min by 100 mM Na sulfate in 20 mM Na, K-phosphate (pH 7.4), approximately 6 hrs for complete elution of urinary peak-forming substances was needed. Retention time of albumin was found to be 22.9 min. To shorten the analytic time, 100 mM Na sulfate in 20 mM Na, K-phosphate (pH 7.4) was first used during a 30 min period for separation of albumin. A mixture of acetonitrile/the above solvent = 3/7 (v/v) was then flushed to wash away the peak-forming substances. By this elution mode, the analytic time could be reduced to 3 hrs. When the validity of this procedure was tested, the detection limit of albumin was 0.04 microgram/injection, and a linearity was observed between 0.2 and 50 micrograms/injection. Rats then received single subcutaneous injections of puromycin aminonucleoside, which is a nephrotoxic agent. The plasma albumin concentrations fell at 5, 10 and 15 days after the administration, and the urinary excretions of albumin rose from the 1st day up to the 15th day. The results denoted that our procedure could be a good evaluative tool for nephrotoxicity studies where albuminuria was manifested.

Albumins↗
PubMed1992

[HIV-1 seropositive hemophilia A complicated by disseminated intravascular coagulation syndrome and acute pancreatitis during treatment of Pneumocystis carinii pneumonia].

Pneumocystis carinii pneumonia (PCP) is a major opportunistic infection in acquired immunodeficiency syndrome (AIDS) and is treated with co-trimoxazole, pentamidine and others. The severe adverse reactions, including bone marrow suppression, by these therapeutic agents often preclude their continued use. A 14-year-old male HIV-positive hemophilia A patient, who was complicated by disseminated intravascular coagulation syndrome (DIC) following acute pancreatitis during treatment for PCP, was treated with proteinase inhibitors and anticoagulant agents. He was improved and discharged. As pentamidine may cause pancreatitis and develop DIC, it is important that pancreatic enzymes should be carefully followed when this agent administrated. In this case, granulocyte colony-stimulating factor and erythropoietin were effective for the bone marrow suppression, suggesting that importance of these agents for the prophylaxis of other secondary infections during the treatment.

AIDS-Related Opportunistic Infections↗
PubMed1991

[A case of subacute idiopathic pure pan-dysautonomia--recovery with prednisolone therapy].

A 52-year-old man had, after an episode of fever in June 1989, developed orthostatic dizziness followed by sexual impotence, dysuria, decreased sweating and weight-loss, which progressed gradually and reached their maximum seven months after the onset. He was given 400 mg of droxydopa and 8 mg of midodrine HCL per day without apparent benefits, and was admitted to our hospital. His blood pressure (mmHg) and heart rate were 167/102 and 68 in supine position, and 74/41 and 62 in sitting position. Skin was dry. Pupillary reactions were sluggish. Left pupil was slightly irregular. Other cranial nerves, sensory and somatic motor functions were normal. Laboratory tests revealed as follows: slight anemia, ESR 42 mm/hour, serum IgG 2236 mg/dl, CSF protein 64 mg/dl and positive tests for non-specific autoantibodies. Nerve conduction studies and electromyogram were normal. Autonomic function tests showed postganglionic impairments of sympathetic and parasympathetic systems. The sural nerve biopsy disclosed normal myelinated fibers, but decreased unmyelinated fiber density to 60% of the control value. No demyelinating lesions, cell infiltration or amyloid deposits were seen. Under the diagnosis of idiopathic pure pan-dysautonomia, prednisolone, initially 60 mg daily, was added. Within 10 days, he showed marked improvement of general conditions. No exacerbation was seen during reduction or after withdrawal of prednisolone. Repeated tests showed normalizing laboratory data and regression of autonomic deficits. A year after onset he regained normal social life.(ABSTRACT TRUNCATED AT 250 WORDS)

Acute Disease↗
PubMed1989

[A case report of meningitis and sepsis due to Enterococcus faecium complicated with strongyloidiasis].

A 80 year-old male was transferred to our department on 18th Aug. 1988, for high fever and clouding of the consciousness. He had been treated with steroid hormone (betamethasone 3.0 mg/day for 15 days) for his uveitis. Enterococcus faecium was isolated from both blood and spinal fluid, and then Strongyloides sterocoralis was revealed both in the sputum and stool. Anti-Human T-cell leukemia virus 1 (HTLV-1) antibody was also positive serologically. At first, beta-lactam antibiotics were used for the treatment of purulent meningitis and sepsis, but after performing sensitivity tests for E. faecium, the antibiotics were changed to rifampicin (RFP), fosfomycin (FOM) and ofloxacin (OFLX) for their excellent activity against the organism. After the clinical symptoms, subsided, thiabendazole was used for disseminated strongyloidiasis in daily doses of 2,500 mg for six days initially. The drug was used three times with two week intervals. Both bacterial and parasite infections subsided and no recurrence has been noticed until now. This is the first case of meningitis caused by E. facium complicated with strongyloidiasis.

Aged↗
PubMed1989

[Two case reports of septic shock due to Vibrio vulnificus with liver cirrhosis].

We have recently experienced a case of Vibrio vulnificus septicemia which occurred in a patient with hepatic cirrhosis, and as we were able to give early antibiotic treatment, the patient survived. We would like to report this case here together with another case experienced 2 years ago. Case 1 was a 58-year-old male who was attending our hospital as an outpatient for hepatic cirrhosis. At 5:30 pm on August 8, 1987, he consumed abalone and giant clam and at 9 pm complained of high fever with shaking chills. He was admitted to our department as an emergency case. Cefoperazone was administered resulting in a decline of fever on the following day. During the course of treatment he fell transiently into pre-DIC, but due mainly to the administration of antibiotics his condition was subsided. Case 2 was a 53-year-old male who was under medical care in our hospital for grave hepatic cirrhosis. On October 11, 1985, he consumed sushi and two days later suffered chills and pyrexia. A blood culture revealed Vibrio vulnificus. His condition improved transiently with administration of Cefazolin, but oliguria, hypotension and ascites occurred subsequently, and finally the patient died on the 22nd day.

Humans↗
PubMed1989

'Delusion of world destruction' (Wetzel). Comparative study between Japan and West Germany.

It has been said that the delusion of world destruction (Weltuntergangserlebnis) in schizophrenia is closely related to Christian culture, and is less frequent in Oriental than in Western countries. Schizophrenic inpatients with delusions of world destruction treated at the Universitäts-Nervenklinik, Tübingen, FRG, were compared with corresponding inpatients at Keio University Hospital and allied hospitals in Japan. As a result, in West Germany, there were 7 (4.7%) patients with delusions of world destruction among 150 schizophrenic patients, accounting for 5.3% of all 131 patients with some delusion. In Japan, 5 (2.8%) of 178 schizophrenic patients had such delusions, accounting for 3.0% of all 166 patients with some delusion. There was no statistically significant difference between the two countries. These incidences were similar to those reported by Kranz (5, 3 and 8% in 1886, 1916 and 1946, respectively) and by Huber and Gross (5.8%). Accordingly, it seems that, although the delusion of world destruction, as a form of world catastrophe, is rare, it occurs among schizophrenic patients at a rather constant incidence, irrespective of the culture or area.

Adult↗
PubMed1989

[A very rare autopsy case of metastatic brain tumor originating from ureteral cancer].

We report here a very rare case of metastatic brain tumor originating from ureteral cancer which was verified at autopsy. An 88-year-old elderly woman was admitted to our department because of recurrent convulsions which started in March, 1987. Following this episode, right hemiparesis and speech disturbance appeared on June 6, 1987. Neurological examinations at admission on August 12, 1987 showed slightly disturbed consciousness, motor dominant aphasia, and dysphasia. Enhanced CT scan demonstrated a large tumor in the left frontal lobe with perifocal edema, and a small mass in the right frontal lobe (Fig. 1). Left carotid angiogram showed downward displacement of the middle cerebral artery complex at the Sylvian portion. After admission, she developed marked distention of the abdomen, with occurrence of hematuria followed by aggravation of respiration and consciousness. She died on September 2, 1987. Removal of metastatic brain tumor had not been performed due to the poor general condition of the patient. An autopsy was permitted. A coronal section of the cerebrum clearly demonstrated a large tumor in the left frontal lobe with small mass in the right frontal lobe (Fig. 2). Kidney and the ureter at the right side showed hydronephrosis and hydroureter because of the presence of ipsilateral ureteral tumor at the distal end (Fig. 3). Microscopical findings verified that the metastatic brain tumor was a transitional cell carcinoma, which was, histologically, completely the same as the ureteral tumor (Fig. 4, 5). No case of metastatic brain tumor originating from ureteral cancer has yet been reported, as far as the authors know.(ABSTRACT TRUNCATED AT 250 WORDS)

Aged↗
PubMed1988

Determination of deoxymononucleotides and deoxynucleosides as a tool for toxicological examination--a trial for conversion of absorbance unit into molar amount in deoxydinucleotide.

Conversion of absorbance unit into molar amount was tried as to deoxydinucleotide. In order to achieve this trial, a reversed-phase high-performance liquid chromatographic method with a linear gradient mode was established for separation and quantification of deoxymononucleotides and deoxynucleosides. Selecting 2'-deoxycytidylyl-(3'-5')-thymidylic (5') acid as an example of deoxydinucleotides, this compound dissolved in water was estimated in absorbance unit at 260 nm, and it was treated by alkaline phosphatase and furthermore by snake venom phosphodiesterase. Based on quantification of the cleaved nucleic acid constituents including 2'-deoxycytidine 5'-monophosphoric acid and thymidine by the above method, the molar amount per 1.000 absorbance unit at 260 nm in the deoxydinucleotide was determined. Thus, the conversion of absorbance unit into molar amount is considered to be applicable to other deoxyoligonucleotides.

Chromatography, High Pressure Liquid↗
PubMed1988

[Mechanism of suppression of colchicine in experimental allergic encephalomyelitis].

Experimental allergic encephalomyelitis(EAE) has been used as an animal model of multiple sclerosis. The establishment of an adoptive transfer system employing encephalitogenic T-cells simplifies the analysis of the pathogenesis of this demyelinating disease. Colchicine, a drug used for gout, has recently been shown to be effective in the treatment of various autoimmune diseases including multiple sclerosis. Moreover, it has been shown that the drug suppresses the development of EAE in SJL mice in vivo. The precise mechanism of this suppression, however, remains unknown. Bearing this in mind, we have attempted to clarify this phenomenon using an adoptive transfer system with encephalitogenic T-cell lines. Lymphocytes from the SJL mice, which had been immunized with bovine myelin basic protein(MBP) and complete Freund's adjuvant were cultured for 4 days with MBP. Following this, the lymphocytes were subcultured in medium containing Con A supernatant(CAS) without antigen. The cells were then stimulated with MBP for 4 days in the presence of antigen presenting cells(APC). Cells were maintained by alternate antigenic stimulation without CAS and propagation in CAS medium over 90 days. The established cell lines were shown to have the cell surface markers of ly-1+, L3t4+, Ly-2-, which are characteristic of T helper/inducer cells. The syngeneic mice receiving these T-cells via tail veins developed neurological deficits in 2 weeks, and inflammatory demyelination was observed in the diseased animals, indicating that the T-cell lines did have encephalitogenicity. Colchicine treatment of the mixture of APC and T-cells in vitro suppressed the proliferation of T-cells, and this was dose dependent.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1988

[Cerebral infarction and subarachnoid hemorrhage caused by dolichoectasia; report of a case].

Dolichoectasia usually causes neurological symptoms because of the pressure it exerts on adjacent intracranial structures and/or impaired circulation distal to ectasia. However, it rarely induces subarachnoid hemorrhage. The authors report just such a rare case of dolichoectasia, which caused subarachnoid hemorrhage following extensive cerebral infarction distal to ectasia. On October 18, 1982, a 47-year-old man came to our hospital with dementia which had started 7 days before, as his chief complaint. Neurological examinations revealed no positive findings. However, plain CT scan showed the presence of small multiple low density areas with a round-shaped high density area at the tip of the right temporal lobe. Angiogram taken on February 18, 1983, revealed that all main cerebral arteries, especially the C1 portion of the right internal carotid artery, and the M1 portion of the right middle cerebral artery were abnormally tortuous and distended. There was also pooling of contrast medium in the distal part of the M1 portion of the right middle cerebral artery which corresponded to the site of high density area on the plain CT scan. On January 31, 1987, he was admitted to our hospital with his chief complaint being the sudden onset of weakness in his left extremities. On admission, he was confused (II-10 on the Japan Coma Scale), dysarthric, and hemiparetic on the left side. Plain CT scan showed extensive cerebral infarction of the right middle cerebral artery territory distal to the ectasia.(ABSTRACT TRUNCATED AT 250 WORDS)

Cerebral Angiography↗