Subcutaneous and muscular abscesses due to Mycobacterium avium intracellulare in a patient with AIDS as a manifestation of immune restoration.
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Biomedical subjects
Publications and source records attributed to M Thibaut.
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X-ray scanning microanalysis was applied to the study of fungal samples such as Sporothrix schenckii. This technique is sufficiently sensitive to detect many elements in the periodical classification of Mendeleev.
Studies were carried out by means of spectrocolorimetry in order to determine the consumption of total lipids in cultures of Trichothecium roseum. This technique has the advantage of requiring very little specimen preparation.
Methods of preparing fungal samples for chemical analysis are briefly considered. The basic principles employed in laser microscopy are outlined. The applications of the technique to mycology and to plant biology are discussed. Such a method allows chemical information to be obtained on the same sample and permits the detection of 74 elements in the periodic system of Mendeleev from lithium (3) to uranium (92). The preparative procedures are evaluated according to general histochemical principles. This technique requires little specimen preparation and has the advantage of not requiring large masses of tissue.
In order to increase our understanding of pathogenic fungi for humans, small pieces extracted from Trichothecium roseum Link were studied, on the one hand, by X-ray microanalysis and, on the other hand, by laser microscopy. The application of these techniques in medical Mycology provides further evidence that they can be used to characterize some elements of Mendeleev's classification.
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Spectroscopic analysis by means of a laser microscope was employed to detect, in fungal tissue, some elements of Mendeleev's classification. Such a method allows all the necessary information to be obtained from the same sample, and permits the detection of 74 elements in the periodic system.
The electron microprobe analyzer was used to study the chemical compositions of some pathogenic fungi for humans. Elementary analysis was based on the characteristic X-ray spectra generated by the electrons passing through the specimens. This is a non-destructive method allowing to perform multiple elementary analysis in the same tissue. In order to increase our understanding of pathogenic fungi, small pieces of fungi tissue were analyzed. The species examined were Histoplasma capsulatum, Blastomyces dermatitidis and Fonsecaea pedrosoi.
Laser microanalysis has been applied to the study of pathogenic fungi. Such a method allows chemical information to be obtained and permits the detection of 74 elements in the periodic system of Mendeleev from lithium (3) to uranium (92).
Laser microscopy was employed to detect some elements of Mendeleev's classification in allergenic moulds for humans. It permits the detection of 74 elements in the periodic system, from lithium (3) to uranium (92). This method requires little specimen preparation and has the advantage of not requiring large masses of tissue.
X-ray microanalysis has been applied to the study of pathogenic fungi for the acquisition of chemical information. The technique of combined scanning electron microscopy and wavelength dispersive spectrometry is described. The chemical analysis depends on the characteristic x-ray spectrum excited by the electrons passing through the sample. This spectrum is analyzed by x-ray wavelength dispersion using crystal spectrometers. All the elements of the periodic system above beryllium can be detected with good sensitivity.
Analytical electron microscopy combines the techniques of high-resolution electron microscopy and high-sensitivity X-ray microanalysis of samples. Spectrometry of the elements (characteristic X-rays produced by a scanning electron microprobe) was employed to detect some elements of Mendeleev's classification in pathogenic fungi for humans. X-ray microanalysis applied in wavelength dispersive spectrometry was used to study Coccidioïdes immitis, Paracoccidioïdes brasiliensis and Trichosporum cutaneum.
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A new apparatus, which combines an electron microprobe and a scanning electron microscope, has been used. This apparatus enabled to conduct a systematic elemental analysis of two species among Aspergillaceae. All elements in the periodic table whose atomic numbers lie between boron and uranium can be detected.
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In Aspergillus fumigatus, X-ray microanalysis applied in wavelength dispersive spectroscopy enabled us to detect the presence of calcium, potassium, sulfur, phosphorus, and magnesium; and in A. niger we detected the presence of calcium, potassium, chloride, sulfur, phosphorus, sodium, and magnesium. These various elements were identified by their K alpha lines.
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